tetano
Editor, Senior Moderator
Cell Rep
. 2021 Jun 8;109305.
doi: 10.1016/j.celrep.2021.109305. Online ahead of print.
Identification of presented SARS-CoV-2 HLA class I and HLA class II peptides using HLA peptidomics
Adi Nagler[SUP] 1 [/SUP], Shelly Kalaora[SUP] 1 [/SUP], Chaya Barbolin[SUP] 1 [/SUP], Anastasia Gangaev[SUP] 2 [/SUP], Steven L C Ketelaars[SUP] 2 [/SUP], Michal Alon[SUP] 1 [/SUP], Joy Pai[SUP] 3 [/SUP], Gil Benedek[SUP] 4 [/SUP], Yfat Yahalom-Ronen[SUP] 5 [/SUP], Noam Erez[SUP] 5 [/SUP], Polina Greenberg[SUP] 1 [/SUP], Gal Yagel[SUP] 1 [/SUP], Aviyah Peri[SUP] 1 [/SUP], Yishai Levin[SUP] 6 [/SUP], Ansuman T Satpathy[SUP] 3 [/SUP], Erez Bar-Haim[SUP] 7 [/SUP], Nir Paran[SUP] 5 [/SUP], Pia Kvistborg[SUP] 4 [/SUP], Yardena Samuels[SUP] 8 [/SUP]
Affiliations
Abstract
The human leukocyte antigen (HLA)-bound viral antigens serve as an immunological signature that can be selectively recognized by T cells. As viruses evolve by acquiring mutations, it is essential to identify a range of presented viral antigens. Using HLA peptidomics, we are able to identify severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-derived peptides presented by highly prevalent HLA class I (HLA-I) molecules by using infected cells as well as overexpression of SARS-CoV-2 genes. We find 26 HLA-I peptides and 36 HLA class II (HLA-II) peptides. Among the identified peptides, some are shared between different cells and some are derived from out-of-frame open reading frames (ORFs). Seven of these peptides were previously shown to be immunogenic, and we identify two additional immunoreactive peptides by using HLA multimer staining. These results may aid the development of the next generation of SARS-CoV-2 vaccines based on presented viral-specific antigens that span several of the viral genes.
Keywords: HLA; Peptides; Peptidomics; SARS-CoV-2; immuno-reactive; out-of-frame-ORFs.
. 2021 Jun 8;109305.
doi: 10.1016/j.celrep.2021.109305. Online ahead of print.
Identification of presented SARS-CoV-2 HLA class I and HLA class II peptides using HLA peptidomics
Adi Nagler[SUP] 1 [/SUP], Shelly Kalaora[SUP] 1 [/SUP], Chaya Barbolin[SUP] 1 [/SUP], Anastasia Gangaev[SUP] 2 [/SUP], Steven L C Ketelaars[SUP] 2 [/SUP], Michal Alon[SUP] 1 [/SUP], Joy Pai[SUP] 3 [/SUP], Gil Benedek[SUP] 4 [/SUP], Yfat Yahalom-Ronen[SUP] 5 [/SUP], Noam Erez[SUP] 5 [/SUP], Polina Greenberg[SUP] 1 [/SUP], Gal Yagel[SUP] 1 [/SUP], Aviyah Peri[SUP] 1 [/SUP], Yishai Levin[SUP] 6 [/SUP], Ansuman T Satpathy[SUP] 3 [/SUP], Erez Bar-Haim[SUP] 7 [/SUP], Nir Paran[SUP] 5 [/SUP], Pia Kvistborg[SUP] 4 [/SUP], Yardena Samuels[SUP] 8 [/SUP]
Affiliations
- PMID: 34166618
- DOI: 10.1016/j.celrep.2021.109305
Abstract
The human leukocyte antigen (HLA)-bound viral antigens serve as an immunological signature that can be selectively recognized by T cells. As viruses evolve by acquiring mutations, it is essential to identify a range of presented viral antigens. Using HLA peptidomics, we are able to identify severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-derived peptides presented by highly prevalent HLA class I (HLA-I) molecules by using infected cells as well as overexpression of SARS-CoV-2 genes. We find 26 HLA-I peptides and 36 HLA class II (HLA-II) peptides. Among the identified peptides, some are shared between different cells and some are derived from out-of-frame open reading frames (ORFs). Seven of these peptides were previously shown to be immunogenic, and we identify two additional immunoreactive peptides by using HLA multimer staining. These results may aid the development of the next generation of SARS-CoV-2 vaccines based on presented viral-specific antigens that span several of the viral genes.
Keywords: HLA; Peptides; Peptidomics; SARS-CoV-2; immuno-reactive; out-of-frame-ORFs.