tetano
Editor, Senior Moderator
Cell Rep Med
. 2020 Sep 22;100121.
doi: 10.1016/j.xcrm.2020.100121. Online ahead of print.
Oral SARS-CoV-2 inoculation establishes subclinical respiratory infection with virus shedding in golden Syrian hamsters
Andrew Chak-Yiu Lee[SUP] 1 [/SUP], Anna Jinxia Zhang[SUP] 1 [/SUP], Jasper Fuk-Woo Chan[SUP] 1 2 [/SUP], Can Li[SUP] 1 [/SUP], Zhimeng Fan[SUP] 1 [/SUP], Feifei Liu[SUP] 1 [/SUP], Yanxia Chen[SUP] 1 [/SUP], Ronghui Liang[SUP] 1 [/SUP], Siddharth Sridhar[SUP] 1 2 [/SUP], Jian-Piao Cai[SUP] 1 [/SUP], Vincent Kwok-Man Poon[SUP] 1 [/SUP], Chris Chung-Sing Chan[SUP] 1 [/SUP], Kelvin Kai-Wang To[SUP] 1 2 [/SUP], Shuofeng Yuan[SUP] 1 [/SUP], Jie Zhou[SUP] 1 [/SUP], Hin Chu[SUP] 1 [/SUP], Kwok-Yung Yuen[SUP] 1 2 [/SUP]
Affiliations
Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is transmitted largely by respiratory droplets or airborne aerosols. Despite being frequently found in the immediate environment and faeces of patients, evidence supporting oral acquisition of SARS-CoV-2 is unavailable. Utilizing Syrian hamster model, we demonstrate that the severity of pneumonia induced by intranasal inhalation of SARS-CoV-2 increases with virus inoculum. SARS-CoV-2 retains its infectivity in vitro in simulated human fed-gastric and fasted-intestinal fluid after two hours. Oral inoculation with the highest intranasal inoculum(10[SUP]5[/SUP]PFU) causes mild pneumonia in 67% (4/6) of the animals with no weight loss. The lung histopathology score and viral load are significantly lower than those infected by the lowest intranasal inoculum(100PFU). However, 83% oral infection (10/12 hamsters) have similar level of detectable viral shedding from oral swabs and faeces as that of intranasally infected hamsters. Our findings indicate oral acquisition of SARS-CoV-2 can establish subclinical respiratory infection with less efficiency.
Keywords: COVID-19; SARS-CoV-2; coronavirus; gastrointestinal; hamster; oral; subclinical.
. 2020 Sep 22;100121.
doi: 10.1016/j.xcrm.2020.100121. Online ahead of print.
Oral SARS-CoV-2 inoculation establishes subclinical respiratory infection with virus shedding in golden Syrian hamsters
Andrew Chak-Yiu Lee[SUP] 1 [/SUP], Anna Jinxia Zhang[SUP] 1 [/SUP], Jasper Fuk-Woo Chan[SUP] 1 2 [/SUP], Can Li[SUP] 1 [/SUP], Zhimeng Fan[SUP] 1 [/SUP], Feifei Liu[SUP] 1 [/SUP], Yanxia Chen[SUP] 1 [/SUP], Ronghui Liang[SUP] 1 [/SUP], Siddharth Sridhar[SUP] 1 2 [/SUP], Jian-Piao Cai[SUP] 1 [/SUP], Vincent Kwok-Man Poon[SUP] 1 [/SUP], Chris Chung-Sing Chan[SUP] 1 [/SUP], Kelvin Kai-Wang To[SUP] 1 2 [/SUP], Shuofeng Yuan[SUP] 1 [/SUP], Jie Zhou[SUP] 1 [/SUP], Hin Chu[SUP] 1 [/SUP], Kwok-Yung Yuen[SUP] 1 2 [/SUP]
Affiliations
- PMID: 32984855
- PMCID: PMC7508015
- DOI: 10.1016/j.xcrm.2020.100121
Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is transmitted largely by respiratory droplets or airborne aerosols. Despite being frequently found in the immediate environment and faeces of patients, evidence supporting oral acquisition of SARS-CoV-2 is unavailable. Utilizing Syrian hamster model, we demonstrate that the severity of pneumonia induced by intranasal inhalation of SARS-CoV-2 increases with virus inoculum. SARS-CoV-2 retains its infectivity in vitro in simulated human fed-gastric and fasted-intestinal fluid after two hours. Oral inoculation with the highest intranasal inoculum(10[SUP]5[/SUP]PFU) causes mild pneumonia in 67% (4/6) of the animals with no weight loss. The lung histopathology score and viral load are significantly lower than those infected by the lowest intranasal inoculum(100PFU). However, 83% oral infection (10/12 hamsters) have similar level of detectable viral shedding from oral swabs and faeces as that of intranasally infected hamsters. Our findings indicate oral acquisition of SARS-CoV-2 can establish subclinical respiratory infection with less efficiency.
Keywords: COVID-19; SARS-CoV-2; coronavirus; gastrointestinal; hamster; oral; subclinical.