tetano
Editor, Senior Moderator
Cell Rep Methods
. 2024 Aug 30:100856.
doi: 10.1016/j.crmeth.2024.100856. Online ahead of print. A quadri-fluorescence SARS-CoV-2 pseudovirus system for efficient antigenic characterization of multiple circulating variants
Jijing Chen[SUP] 1 [/SUP], Zehong Huang[SUP] 1 [/SUP], Jin Xiao[SUP] 1 [/SUP], Shuangling Du[SUP] 1 [/SUP], Qingfang Bu[SUP] 1 [/SUP], Huilin Guo[SUP] 1 [/SUP], Jianghui Ye[SUP] 1 [/SUP], Shiqi Chen[SUP] 2 [/SUP], Jiahua Gao[SUP] 1 [/SUP], Zonglin Li[SUP] 1 [/SUP], Miaolin Lan[SUP] 1 [/SUP], Shaojuan Wang[SUP] 1 [/SUP], Tianying Zhang[SUP] 1 [/SUP], Jiming Zhang[SUP] 2 [/SUP], Yangtao Wu[SUP] 3 [/SUP], Yali Zhang[SUP] 4 [/SUP], Ningshao Xia[SUP] 1 [/SUP], Quan Yuan[SUP] 5 [/SUP], Tong Cheng[SUP] 6 [/SUP]
Affiliations
The ongoing co-circulation of multiple severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) strains necessitates advanced methods such as high-throughput multiplex pseudovirus systems for evaluating immune responses to different variants, crucial for developing updated vaccines and neutralizing antibodies (nAbs). We have developed a quadri-fluorescence (qFluo) pseudovirus platform by four fluorescent reporters with different spectra, allowing simultaneous measurement of the nAbs against four variants in a single test. qFluo shows high concordance with the classical single-reporter assay when testing monoclonal antibodies and human plasma. Utilizing qFluo, we assessed the immunogenicities of the spike of BA.5, BQ.1.1, XBB.1.5, and CH.1.1 in hamsters. An analysis of cross-neutralization against 51 variants demonstrated superior protective immunity from XBB.1.5, especially against prevalent strains such as "FLip" and JN.1, compared to BA.5. Our finding partially fills the knowledge gap concerning the immunogenic efficacy of the XBB.1.5 vaccine against current dominant variants, being instrumental in vaccine-strain decisions and insight into the evolutionary path of SARS-CoV-2.
Keywords: BA.2.86; CP: Immunology; CP: Microbiology; FLip; JN.1; Omicron; SARS-CoV-2; antibody evasion; cell-based assay; high throughput; neutralization.
. 2024 Aug 30:100856.
doi: 10.1016/j.crmeth.2024.100856. Online ahead of print. A quadri-fluorescence SARS-CoV-2 pseudovirus system for efficient antigenic characterization of multiple circulating variants
Jijing Chen[SUP] 1 [/SUP], Zehong Huang[SUP] 1 [/SUP], Jin Xiao[SUP] 1 [/SUP], Shuangling Du[SUP] 1 [/SUP], Qingfang Bu[SUP] 1 [/SUP], Huilin Guo[SUP] 1 [/SUP], Jianghui Ye[SUP] 1 [/SUP], Shiqi Chen[SUP] 2 [/SUP], Jiahua Gao[SUP] 1 [/SUP], Zonglin Li[SUP] 1 [/SUP], Miaolin Lan[SUP] 1 [/SUP], Shaojuan Wang[SUP] 1 [/SUP], Tianying Zhang[SUP] 1 [/SUP], Jiming Zhang[SUP] 2 [/SUP], Yangtao Wu[SUP] 3 [/SUP], Yali Zhang[SUP] 4 [/SUP], Ningshao Xia[SUP] 1 [/SUP], Quan Yuan[SUP] 5 [/SUP], Tong Cheng[SUP] 6 [/SUP]
Affiliations
- PMID: 39243752
- DOI: 10.1016/j.crmeth.2024.100856
The ongoing co-circulation of multiple severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) strains necessitates advanced methods such as high-throughput multiplex pseudovirus systems for evaluating immune responses to different variants, crucial for developing updated vaccines and neutralizing antibodies (nAbs). We have developed a quadri-fluorescence (qFluo) pseudovirus platform by four fluorescent reporters with different spectra, allowing simultaneous measurement of the nAbs against four variants in a single test. qFluo shows high concordance with the classical single-reporter assay when testing monoclonal antibodies and human plasma. Utilizing qFluo, we assessed the immunogenicities of the spike of BA.5, BQ.1.1, XBB.1.5, and CH.1.1 in hamsters. An analysis of cross-neutralization against 51 variants demonstrated superior protective immunity from XBB.1.5, especially against prevalent strains such as "FLip" and JN.1, compared to BA.5. Our finding partially fills the knowledge gap concerning the immunogenic efficacy of the XBB.1.5 vaccine against current dominant variants, being instrumental in vaccine-strain decisions and insight into the evolutionary path of SARS-CoV-2.
Keywords: BA.2.86; CP: Immunology; CP: Microbiology; FLip; JN.1; Omicron; SARS-CoV-2; antibody evasion; cell-based assay; high throughput; neutralization.