tetano
Editor, Senior Moderator
Cells
. 2021 Dec 27;11(1):68.
doi: 10.3390/cells11010068.
Analysis of TCR Repertoire by High-Throughput Sequencing Indicates the Feature of T Cell Immune Response after SARS-CoV-2 Infection
Yifan Wang[SUP] 1 2 3 [/SUP], Fugang Duan[SUP] 1 2 3 [/SUP], Zhu Zhu[SUP] 1 2 3 [/SUP], Meng Yu[SUP] 1 2 3 [/SUP], Xiaodong Jia[SUP] 4 [/SUP], Hui Dai[SUP] 1 2 3 [/SUP], Pingzhang Wang[SUP] 1 2 3 [/SUP], Xiaoyan Qiu[SUP] 1 2 3 [/SUP], Yinying Lu[SUP] 4 [/SUP], Jing Huang[SUP] 1 2 3 [/SUP]
Affiliations
Abstract
Coronavirus disease 2019 (COVID-19) is a global infectious disease caused by the SARS-CoV-2 coronavirus. T cells play an essential role in the body's fighting against the virus invasion, and the T cell receptor (TCR) is crucial in T cell-mediated virus recognition and clearance. However, little has been known about the features of T cell response in convalescent COVID-19 patients. In this study, using 5'RACE technology and PacBio sequencing, we analyzed the TCR repertoire of COVID-19 patients after recovery for 2 weeks and 6 months compared with the healthy donors. The TCR clustering and CDR3 annotation were exploited to discover groups of patient-specific TCR clonotypes with potential SARS-CoV-2 antigen specificities. We first identified CD4[SUP]+[/SUP] and CD8[SUP]+[/SUP] T cell clones with certain clonal expansion after infection, and then observed the preferential recombination usage of V(D) J gene segments in CD4[SUP]+[/SUP] and CD8[SUP]+[/SUP] T cells of COVID-19 patients with different convalescent stages. More important, the TRBV6-5-TRBD2-TRBJ2-7 combination with high frequency was shared between CD4[SUP]+[/SUP] T and CD8[SUP]+[/SUP] T cells of different COVID-19 patients. Finally, we found the dominant characteristic motifs of the CDR3 sequence between recovered COVID-19 and healthy control. Our study provides novel insights on TCR in COVID-19 with different convalescent phases, contributing to our understanding of the immune response induced by SARS-CoV-2.
Keywords: CDR3; COVID-19; SARS-CoV-2; T cell receptor; immune memory.
. 2021 Dec 27;11(1):68.
doi: 10.3390/cells11010068.
Analysis of TCR Repertoire by High-Throughput Sequencing Indicates the Feature of T Cell Immune Response after SARS-CoV-2 Infection
Yifan Wang[SUP] 1 2 3 [/SUP], Fugang Duan[SUP] 1 2 3 [/SUP], Zhu Zhu[SUP] 1 2 3 [/SUP], Meng Yu[SUP] 1 2 3 [/SUP], Xiaodong Jia[SUP] 4 [/SUP], Hui Dai[SUP] 1 2 3 [/SUP], Pingzhang Wang[SUP] 1 2 3 [/SUP], Xiaoyan Qiu[SUP] 1 2 3 [/SUP], Yinying Lu[SUP] 4 [/SUP], Jing Huang[SUP] 1 2 3 [/SUP]
Affiliations
- PMID: 35011632
- DOI: 10.3390/cells11010068
Abstract
Coronavirus disease 2019 (COVID-19) is a global infectious disease caused by the SARS-CoV-2 coronavirus. T cells play an essential role in the body's fighting against the virus invasion, and the T cell receptor (TCR) is crucial in T cell-mediated virus recognition and clearance. However, little has been known about the features of T cell response in convalescent COVID-19 patients. In this study, using 5'RACE technology and PacBio sequencing, we analyzed the TCR repertoire of COVID-19 patients after recovery for 2 weeks and 6 months compared with the healthy donors. The TCR clustering and CDR3 annotation were exploited to discover groups of patient-specific TCR clonotypes with potential SARS-CoV-2 antigen specificities. We first identified CD4[SUP]+[/SUP] and CD8[SUP]+[/SUP] T cell clones with certain clonal expansion after infection, and then observed the preferential recombination usage of V(D) J gene segments in CD4[SUP]+[/SUP] and CD8[SUP]+[/SUP] T cells of COVID-19 patients with different convalescent stages. More important, the TRBV6-5-TRBD2-TRBJ2-7 combination with high frequency was shared between CD4[SUP]+[/SUP] T and CD8[SUP]+[/SUP] T cells of different COVID-19 patients. Finally, we found the dominant characteristic motifs of the CDR3 sequence between recovered COVID-19 and healthy control. Our study provides novel insights on TCR in COVID-19 with different convalescent phases, contributing to our understanding of the immune response induced by SARS-CoV-2.
Keywords: CDR3; COVID-19; SARS-CoV-2; T cell receptor; immune memory.