tetano
Editor, Senior Moderator
Cells
. 2022 Sep 22;11(19):2957.
doi: 10.3390/cells11192957.
Bioinformatics and Functional Analysis of a New Nuclear Localization Sequence of the Influenza A Virus Nucleoprotein
Nhan L T Nguyen[SUP] 1 [/SUP], Nelly Panté[SUP] 1 [/SUP]
Affiliations
Abstract
Influenza viruses deliver their genome into the nucleus of infected cells for replication. This process is mediated by the viral nucleoprotein (NP), which contains two nuclear localization sequences (NLSs): NLS1 at the N-terminus and a recently identified NLS2 ([SUP]212[/SUP]GRKTR[SUP]216[/SUP]). Through mutagenesis and functional studies, we demonstrated that NP must have both NLSs for an efficient nuclear import. As with other NLSs, there may be variations in the basic residues of NLS2 in different strains of the virus, which may affect the nuclear import of the viral genome. Although all NLS2 variants fused to the GFP mediated nuclear import of GFP, bioinformatics showed that 98.8% of reported NP sequences contained either the wild-type sequence [SUP]212[/SUP]GRKTR[SUP]216[/SUP] or [SUP]212[/SUP]GRRTR[SUP]216[/SUP]. Bioinformatics analyses used to study the presence of NLS2 variants in other viral and nuclear proteins resulted in very low hits, with only 0.4% of human nuclear proteins containing putative NLS2. From these, we studied the nucleolar protein 14 (NOP14) and found that NLS2 does not play a role in the nuclear import of this protein but in its nucleolar localization. We also discovered a functional NLS at the C-terminus of NOP14. Our findings indicate that NLS2 is a highly conserved influenza A NP sequence.
Keywords: NLS; NOP14; influenza A virus; nuclear import; nuclear localization sequence; nucleolar protein 14; nucleoprotein.
. 2022 Sep 22;11(19):2957.
doi: 10.3390/cells11192957.
Bioinformatics and Functional Analysis of a New Nuclear Localization Sequence of the Influenza A Virus Nucleoprotein
Nhan L T Nguyen[SUP] 1 [/SUP], Nelly Panté[SUP] 1 [/SUP]
Affiliations
- PMID: 36230922
- DOI: 10.3390/cells11192957
Abstract
Influenza viruses deliver their genome into the nucleus of infected cells for replication. This process is mediated by the viral nucleoprotein (NP), which contains two nuclear localization sequences (NLSs): NLS1 at the N-terminus and a recently identified NLS2 ([SUP]212[/SUP]GRKTR[SUP]216[/SUP]). Through mutagenesis and functional studies, we demonstrated that NP must have both NLSs for an efficient nuclear import. As with other NLSs, there may be variations in the basic residues of NLS2 in different strains of the virus, which may affect the nuclear import of the viral genome. Although all NLS2 variants fused to the GFP mediated nuclear import of GFP, bioinformatics showed that 98.8% of reported NP sequences contained either the wild-type sequence [SUP]212[/SUP]GRKTR[SUP]216[/SUP] or [SUP]212[/SUP]GRRTR[SUP]216[/SUP]. Bioinformatics analyses used to study the presence of NLS2 variants in other viral and nuclear proteins resulted in very low hits, with only 0.4% of human nuclear proteins containing putative NLS2. From these, we studied the nucleolar protein 14 (NOP14) and found that NLS2 does not play a role in the nuclear import of this protein but in its nucleolar localization. We also discovered a functional NLS at the C-terminus of NOP14. Our findings indicate that NLS2 is a highly conserved influenza A NP sequence.
Keywords: NLS; NOP14; influenza A virus; nuclear import; nuclear localization sequence; nucleolar protein 14; nucleoprotein.