tetano
Editor, Senior Moderator
Cells
. 2022 Jan 30;11(3):487.
doi: 10.3390/cells11030487.
Competitive Endogenous RNA Network Activates Host Immune Response in SARS-CoV-2-, panH1N1 (A/California/07/2009)-, and H7N9 (A/Shanghai/1/2013)-Infected Cells
Minghui Yang[SUP] 1 [/SUP], Jin Li[SUP] 2 [/SUP], Shoulong Deng[SUP] 3 [/SUP], Hao Fan[SUP] 4 [/SUP], Yun Peng[SUP] 1 [/SUP], Guoguo Ye[SUP] 1 [/SUP], Jun Wang[SUP] 1 [/SUP], Jinli Wei[SUP] 1 [/SUP], Xiao Jiang[SUP] 1 [/SUP], Zhixiang Xu[SUP] 1 [/SUP], Ling Qing[SUP] 1 [/SUP], Fuxiang Wang[SUP] 1 [/SUP], Yang Yang[SUP] 1 [/SUP], Yingxia Liu[SUP] 1 [/SUP]
Affiliations
Abstract
The global outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is still ongoing, as is research on the molecular mechanisms underlying cellular infection by coronaviruses, with the hope of developing therapeutic agents against this pandemic. Other important respiratory viruses such as 2009 pandemic H1N1 and H7N9 avian influenza virus (AIV), influenza A viruses, are also responsible for a possible outbreak due to their respiratory susceptibility. However, the interaction of these viruses with host cells and the regulation of post-transcriptional genes remains unclear. In this study, we detected and analyzed the comparative transcriptome profiling of SARS-CoV-2, panH1N1 (A/California/07/2009), and H7N9 (A/Shanghai/1/2013) infected cells. The results showed that the commonly upregulated genes among the three groups were mainly involved in autophagy, pertussis, and tuberculosis, which indicated that autophagy plays an important role in viral pathogenicity. There are three groups of commonly downregulated genes involved in metabolic pathways. Notably, unlike panH1N1 and H7N9, SARS-CoV-2 infection can inhibit the m-TOR pathway and activate the p53 signaling pathway, which may be responsible for unique autophagy induction and cell apoptosis. Particularly, upregulated expression of IRF1 was found in SARS-CoV-2, panH1N1, and H7N9 infection. Further analysis showed SARS-CoV-2, panH1N1, and H7N9 infection-induced upregulation of lncRNA-34087.27 could serve as a competitive endogenous RNA to stabilize IRF1 mRNA by competitively binding with miR-302b-3p. This study provides new insights into the molecular mechanisms of influenza A virus and SARS-CoV-2 infection.
Keywords: IRF1; SARS-CoV-2; autophagy; influenza A; noncoding RNA.
. 2022 Jan 30;11(3):487.
doi: 10.3390/cells11030487.
Competitive Endogenous RNA Network Activates Host Immune Response in SARS-CoV-2-, panH1N1 (A/California/07/2009)-, and H7N9 (A/Shanghai/1/2013)-Infected Cells
Minghui Yang[SUP] 1 [/SUP], Jin Li[SUP] 2 [/SUP], Shoulong Deng[SUP] 3 [/SUP], Hao Fan[SUP] 4 [/SUP], Yun Peng[SUP] 1 [/SUP], Guoguo Ye[SUP] 1 [/SUP], Jun Wang[SUP] 1 [/SUP], Jinli Wei[SUP] 1 [/SUP], Xiao Jiang[SUP] 1 [/SUP], Zhixiang Xu[SUP] 1 [/SUP], Ling Qing[SUP] 1 [/SUP], Fuxiang Wang[SUP] 1 [/SUP], Yang Yang[SUP] 1 [/SUP], Yingxia Liu[SUP] 1 [/SUP]
Affiliations
- PMID: 35159296
- DOI: 10.3390/cells11030487
Abstract
The global outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is still ongoing, as is research on the molecular mechanisms underlying cellular infection by coronaviruses, with the hope of developing therapeutic agents against this pandemic. Other important respiratory viruses such as 2009 pandemic H1N1 and H7N9 avian influenza virus (AIV), influenza A viruses, are also responsible for a possible outbreak due to their respiratory susceptibility. However, the interaction of these viruses with host cells and the regulation of post-transcriptional genes remains unclear. In this study, we detected and analyzed the comparative transcriptome profiling of SARS-CoV-2, panH1N1 (A/California/07/2009), and H7N9 (A/Shanghai/1/2013) infected cells. The results showed that the commonly upregulated genes among the three groups were mainly involved in autophagy, pertussis, and tuberculosis, which indicated that autophagy plays an important role in viral pathogenicity. There are three groups of commonly downregulated genes involved in metabolic pathways. Notably, unlike panH1N1 and H7N9, SARS-CoV-2 infection can inhibit the m-TOR pathway and activate the p53 signaling pathway, which may be responsible for unique autophagy induction and cell apoptosis. Particularly, upregulated expression of IRF1 was found in SARS-CoV-2, panH1N1, and H7N9 infection. Further analysis showed SARS-CoV-2, panH1N1, and H7N9 infection-induced upregulation of lncRNA-34087.27 could serve as a competitive endogenous RNA to stabilize IRF1 mRNA by competitively binding with miR-302b-3p. This study provides new insights into the molecular mechanisms of influenza A virus and SARS-CoV-2 infection.
Keywords: IRF1; SARS-CoV-2; autophagy; influenza A; noncoding RNA.