tetano
Editor, Senior Moderator
Cells
. 2021 Feb 10;10(2):364.
doi: 10.3390/cells10020364.
Molecular Insights into the Thrombotic and Microvascular Injury in Placental Endothelium of Women with Mild or Severe COVID-19
Arturo Flores-Pliego[SUP] 1 [/SUP], Jael Miranda[SUP] 2 [/SUP], Sara Vega-Torreblanca[SUP] 2 [/SUP], Yolotzin Valdespino-V?zquez[SUP] 3 [/SUP], Cecilia Helguera-Repetto[SUP] 1 [/SUP], Aurora Espejel-Nu?ez[SUP] 1 [/SUP], H?ctor Borboa-Olivares[SUP] 4 [/SUP], Salvador Espino Y Sosa[SUP] 5 [/SUP], Paloma Mateu-Rogell[SUP] 5 [/SUP], Mois?s Le?n-Ju?rez[SUP] 1 [/SUP], Victor Ram?rez-Santes[SUP] 6 [/SUP], Arturo Cardona-P?rez[SUP] 7 [/SUP], Isabel Villegas-Mota[SUP] 8 [/SUP], Johnatan Torres-Torres[SUP] 9 [/SUP], ?ngeles Ju?rez-Reyes[SUP] 9 [/SUP], Thelma Rizo-Pica[SUP] 9 [/SUP], Rosa O Gonz?lez[SUP] 10 [/SUP], Lorenza Gonz?lez-Mariscal[SUP] 2 [/SUP], Guadalupe Estrada-Gutierrez[SUP] 11 [/SUP]
Affiliations
Abstract
Clinical manifestations of coronavirus disease 2019 (COVID-19) in pregnant women are diverse, and little is known of the impact of the disease on placental physiology. Severe acute respiratory syndrome coronavirus (SARS-CoV-2) has been detected in the human placenta, and its binding receptor ACE2 is present in a variety of placental cells, including endothelium. Here, we analyze the impact of COVID-19 in placental endothelium, studying by immunofluorescence the expression of von Willebrand factor (vWf), claudin-5, and vascular endothelial (VE) cadherin in the decidua and chorionic villi of placentas from women with mild and severe COVID-19 in comparison to healthy controls. Our results indicate that: (1) vWf expression increases in the endothelium of decidua and chorionic villi of placentas derived from women with COVID-19, being higher in severe cases; (2) Claudin-5 and VE-cadherin expression decrease in the decidua and chorionic villus of placentas from women with severe COVID-19 but not in those with mild disease. Placental histological analysis reveals thrombosis, infarcts, and vascular wall remodeling, confirming the deleterious effect of COVID-19 on placental vessels. Together, these results suggest that placentas from women with COVID-19 have a condition of leaky endothelium and thrombosis, which is sensitive to disease severity.
Keywords: COVID-19; VE-cadherin; claudin-5; endothelium; placenta; von Willebrand factor.
. 2021 Feb 10;10(2):364.
doi: 10.3390/cells10020364.
Molecular Insights into the Thrombotic and Microvascular Injury in Placental Endothelium of Women with Mild or Severe COVID-19
Arturo Flores-Pliego[SUP] 1 [/SUP], Jael Miranda[SUP] 2 [/SUP], Sara Vega-Torreblanca[SUP] 2 [/SUP], Yolotzin Valdespino-V?zquez[SUP] 3 [/SUP], Cecilia Helguera-Repetto[SUP] 1 [/SUP], Aurora Espejel-Nu?ez[SUP] 1 [/SUP], H?ctor Borboa-Olivares[SUP] 4 [/SUP], Salvador Espino Y Sosa[SUP] 5 [/SUP], Paloma Mateu-Rogell[SUP] 5 [/SUP], Mois?s Le?n-Ju?rez[SUP] 1 [/SUP], Victor Ram?rez-Santes[SUP] 6 [/SUP], Arturo Cardona-P?rez[SUP] 7 [/SUP], Isabel Villegas-Mota[SUP] 8 [/SUP], Johnatan Torres-Torres[SUP] 9 [/SUP], ?ngeles Ju?rez-Reyes[SUP] 9 [/SUP], Thelma Rizo-Pica[SUP] 9 [/SUP], Rosa O Gonz?lez[SUP] 10 [/SUP], Lorenza Gonz?lez-Mariscal[SUP] 2 [/SUP], Guadalupe Estrada-Gutierrez[SUP] 11 [/SUP]
Affiliations
- PMID: 33578631
- DOI: 10.3390/cells10020364
Abstract
Clinical manifestations of coronavirus disease 2019 (COVID-19) in pregnant women are diverse, and little is known of the impact of the disease on placental physiology. Severe acute respiratory syndrome coronavirus (SARS-CoV-2) has been detected in the human placenta, and its binding receptor ACE2 is present in a variety of placental cells, including endothelium. Here, we analyze the impact of COVID-19 in placental endothelium, studying by immunofluorescence the expression of von Willebrand factor (vWf), claudin-5, and vascular endothelial (VE) cadherin in the decidua and chorionic villi of placentas from women with mild and severe COVID-19 in comparison to healthy controls. Our results indicate that: (1) vWf expression increases in the endothelium of decidua and chorionic villi of placentas derived from women with COVID-19, being higher in severe cases; (2) Claudin-5 and VE-cadherin expression decrease in the decidua and chorionic villus of placentas from women with severe COVID-19 but not in those with mild disease. Placental histological analysis reveals thrombosis, infarcts, and vascular wall remodeling, confirming the deleterious effect of COVID-19 on placental vessels. Together, these results suggest that placentas from women with COVID-19 have a condition of leaky endothelium and thrombosis, which is sensitive to disease severity.
Keywords: COVID-19; VE-cadherin; claudin-5; endothelium; placenta; von Willebrand factor.