tetano
Editor, Senior Moderator
J Gen Virol. 2013 Nov 16. doi: 10.1099/vir.0.058438-0. [Epub ahead of print]
Characterization of innate responses to influenza virus infection in a novel lung type I epithelial cell model.
Rosenberger CM, Podyminogin RL, Askovich PS, Navarro G, Kaiser SM, Sanders CJ, McClaren JL, Tam VC, Dash P, Noonan JG, Jones BG, Surman SL, Peschon JJ, Diercks AH, Hurwitz JL, Doherty PC, Thomas PG, Aderem A.
Source
Genentech, Inc.;
Abstract
Type I alveolar epithelial cells are a replicative niche for influenza in vivo, yet their response to infection is not fully understood. To better characterize their cellular responses, we have created an immortalized murine lung epithelial type I cell line (LET1). These cells support spreading influenza virus infection in the absence of exogenous protease, and thus permit simultaneous analysis of viral replication dynamics and host cell responses. LET1 cells can be productively infected with human, swine, and mouse-adapted strains of influenza virus and exhibit expression of an antiviral transcriptional program and robust cytokine secretion. We characterized influenza virus replication dynamics and host responses of lung type I epithelial cells and identified the capacity of epithelial cell-derived type I interferon to regulate specific modules of antiviral effectors to establish an effective antiviral state. Together, our results indicate that the type I epithelial cell can play a major role in restricting influenza virus infection without contribution from the hematopoietic compartment.
KEYWORDS:
Host response, Influenza, Innate immunity, Lung infection
PMID:
24243730
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24243730
Characterization of innate responses to influenza virus infection in a novel lung type I epithelial cell model.
Rosenberger CM, Podyminogin RL, Askovich PS, Navarro G, Kaiser SM, Sanders CJ, McClaren JL, Tam VC, Dash P, Noonan JG, Jones BG, Surman SL, Peschon JJ, Diercks AH, Hurwitz JL, Doherty PC, Thomas PG, Aderem A.
Source
Genentech, Inc.;
Abstract
Type I alveolar epithelial cells are a replicative niche for influenza in vivo, yet their response to infection is not fully understood. To better characterize their cellular responses, we have created an immortalized murine lung epithelial type I cell line (LET1). These cells support spreading influenza virus infection in the absence of exogenous protease, and thus permit simultaneous analysis of viral replication dynamics and host cell responses. LET1 cells can be productively infected with human, swine, and mouse-adapted strains of influenza virus and exhibit expression of an antiviral transcriptional program and robust cytokine secretion. We characterized influenza virus replication dynamics and host responses of lung type I epithelial cells and identified the capacity of epithelial cell-derived type I interferon to regulate specific modules of antiviral effectors to establish an effective antiviral state. Together, our results indicate that the type I epithelial cell can play a major role in restricting influenza virus infection without contribution from the hematopoietic compartment.
KEYWORDS:
Host response, Influenza, Innate immunity, Lung infection
PMID:
24243730
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24243730