tetano
Editor, Senior Moderator
Chemistry
. 2022 Feb 14.
doi: 10.1002/chem.202104054. Online ahead of print.
Rapid and high-throughput SARS-CoV-2 RNA detection without RNA extraction and amplification using a microfluidic biochip
Yujin Chu[SUP] 1 [/SUP], Jiaoyan Qiu[SUP] 2 [/SUP], Yihe Wang[SUP] 2 [/SUP], Min Wang[SUP] 2 [/SUP], Yu Zhang[SUP] 2 [/SUP], Lin Han[SUP] 3 [/SUP]
Affiliations
Abstract
The ongoing outbreak of the severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) has spread globally and poses a threat to public health and national economic development. Rapid and high-throughput SARS-CoV-2 RNA detection without the need of RNA extraction and amplification remain a key challenge. In this study, we developed a novel SARS-CoV-2 RNA detection strategy using a microfluidic biochip for the rapid and ultrasensitive detection of SARS-CoV-2 without RNA extraction and amplification. The developed strategy takes advantage of the specific SARS-CoV-2 RNA and probe DNA reaction in the microfluidic channel, fluorescence signal regulation by nanomaterials, and accurate sample control by the microfluidic chip. It presents an ultralow limit of detection of 600 copies/mL in a large linear detection regime from 1 aM to 100 fM. Fifteen samples were simultaneously detected in 40 min without the need for RNA purification and amplification. The detection accuracy of the strategy was validated via quantitative reverse transcription-polymerase chain reaction (qRT-PCR), with a recovery of 99-113%. Therefore, the SARS-CoV-2 RNA detection strategy proposed in this study can potentially be used for the quantitative diagnosis of viral infectious diseases.
Keywords: Amplification-free; Nucleic acids; RNA purification-free; Rapid & Ultrasensitive; biosensors.
. 2022 Feb 14.
doi: 10.1002/chem.202104054. Online ahead of print.
Rapid and high-throughput SARS-CoV-2 RNA detection without RNA extraction and amplification using a microfluidic biochip
Yujin Chu[SUP] 1 [/SUP], Jiaoyan Qiu[SUP] 2 [/SUP], Yihe Wang[SUP] 2 [/SUP], Min Wang[SUP] 2 [/SUP], Yu Zhang[SUP] 2 [/SUP], Lin Han[SUP] 3 [/SUP]
Affiliations
- PMID: 35165963
- DOI: 10.1002/chem.202104054
Abstract
The ongoing outbreak of the severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) has spread globally and poses a threat to public health and national economic development. Rapid and high-throughput SARS-CoV-2 RNA detection without the need of RNA extraction and amplification remain a key challenge. In this study, we developed a novel SARS-CoV-2 RNA detection strategy using a microfluidic biochip for the rapid and ultrasensitive detection of SARS-CoV-2 without RNA extraction and amplification. The developed strategy takes advantage of the specific SARS-CoV-2 RNA and probe DNA reaction in the microfluidic channel, fluorescence signal regulation by nanomaterials, and accurate sample control by the microfluidic chip. It presents an ultralow limit of detection of 600 copies/mL in a large linear detection regime from 1 aM to 100 fM. Fifteen samples were simultaneously detected in 40 min without the need for RNA purification and amplification. The detection accuracy of the strategy was validated via quantitative reverse transcription-polymerase chain reaction (qRT-PCR), with a recovery of 99-113%. Therefore, the SARS-CoV-2 RNA detection strategy proposed in this study can potentially be used for the quantitative diagnosis of viral infectious diseases.
Keywords: Amplification-free; Nucleic acids; RNA purification-free; Rapid & Ultrasensitive; biosensors.