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China - H7N9 found in chickens says Harbin Veterinary Research Institute: wet markets and farms

sharon sanders

Editor-in-Chief & President
machine translation

Mainland experts: H7N9 from poultry
04 - 24 00:03


Shandong diagnosed the first case of human infection with the H7N9 avian influenza.
(Roundup) (Sing Tao Daily reported) Mainland experts yesterday the latest research from the live poultry market surveillance more than 200 poultry and environmental samples, nearly 10% with the H7N9 virus, the virus genes and human viruses of H7N9 avian influenza highly homologous genes, may be the source of human infection with H7N9 viral genes see partial amino acid mutations, increased upper respiratory tract infection and the risk of human-to-human transmission. Mainland yesterday added four new cases, Shandong Province, the first in the move.
Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences research team, earlier in Shanghai, Anhui live poultry markets and farms, poultry and environmental samples collected nine hundred and seventy, Anhui and farms samples were found with the H7N9 virus,

the only a live poultry market in Shanghai, the 280 samples the twenty found with the H7N9 virus, the detection rate of about seven percent, including 10 chicken samples, seven environmental samples and three pigeons samples of in the move, which is to recruit the highest proportion of environmental samples, but all Ducks samples of thirty, failed to reveal the H7N9 virus.

Tsang Tak-yin, on behalf of the Hong Kong Society for Infectious Diseases, analyze the results, that the live poultry market in Shanghai is not difficult to find the virus, that is, about every ten samples there is a stained virus, especially in environmental samples, the situation is a cause for concern.
The study refers to the H7N9 avian influenza virus gene fragments were highly homologous to that of human infection with the H7N9 virus, possibly from a batch of poultry H7N9 avian influenza virus H7N9 human isolates. Viral genetic analysis of the H7N9 gene amino acids Q226L mutations easy it is caused by respiratory infection.
Tsang Tak Yin explained that H7N9 infection of the upper respiratory tract, the H5N1 before lower respiratory tract infection, relatively H7N9 gathered in the upper respiratory tract, spread through coughing, sneezing, have a higher risk of human-to-human transmission.
In addition, the mainland yesterday soared to four H7N9 confirmed cases were from Zhejiang, Anhui and Shandong cases for the first time, a total of $ 08. The Shandong case involved a 36-year-old man, patients hospitalized with severe pneumonia on Sunday night, in the hospital for treatment, is now in critical condition, his nine close contacts there have been no related infection symptoms.
Zhejiang two new cases involving two 61-year-old and 80-year-old man, also in critical condition. Anhui case involved a 92-year-old man, is now in serious condition. Diagnosed patients yesterday added a spirits of the dead, on Wednesday confirmed Hangzhou, Zhejiang 80-year-old male patient, after she was certified dead.
The Hong Kong of Health Centre for Health Protection has not received yesterday H7 suspected cases. In Hong Kong recently seized a dead bird in figures continued to rise, on the 20th of this month seized ninety-two in the 21st seized fifty-nine, the day before yesterday, one hundred and one. Yesterday seized the dead bird in Tin Shui Wai, Sai Kung and Cheung Sha Wan.

zhttp://news.sina.com.hk/news/20130424/-2-2951894/1.html
 
Re: China - H7N9 found in chickens says Harbin Veterinary Research Institute: wet markets to farms

Re: China - H7N9 found in chickens says Harbin Veterinary Research Institute: wet markets to farms

Same story by Sina 5 hours later. Now says all poultry farms tested negative and the fear is that the live poultry markets might contaminate the farms. So how do the chickens in the wet markets acquire H7N9 if not from the farms from where they came?

machine translation

National Laboratory: sowing poison hotbed of live poultry markets
04 - 24 05:00

Ming Pao] H7N9 avian influenza epidemic Mainland yesterday 3 people diagnosed cases rose to 108, the cumulative total of 22 people were killed.

National Avian Influenza Reference Laboratory studies indicate that attack the H7N9 avian influenza virus, the H7N9 virus detected with the live bird markets are highly homologous, with an estimated live bird markets are sowing poison hotbed.

The studies suggest that live bird market H7N9 virus detection and management system should be strengthened to prevent the virus spread to poultry farms by the market.

Visit the Mainland, WHO Assistant Director-General (Health Safety and Environmental Affairs Assistant) Keiji Fukuda told reporters in Shanghai yesterday on available information, the infection is from the live poultry market, the Shanghai municipal government to take prompt measures, the authorities are carried out on patients Blood type school survey, in order to understand the possibility of human-to-human transmission, are not yet clear assessment of whether there is limited human-to-human transmission.
Shanghai from 21 to the present three-day series no new infection cases. The authority of the avian flu, Professor Guan Yi, director of the Hong Kong Dah Sing Infectious Diseases State Key Laboratory of two weeks ago, the newspaper said, with an estimated spread of H7N9 virus from live chickens in the 6th of this month, Shanghai has been closed containment of the live bird markets, the epidemic is very will soon be going downhill.
Yesterday to three people diagnosed accumulated 108
March 31 in Shanghai, Anhui infected with the H7N9 virus, the National Avian Influenza Reference Laboratory aging orchid research team, collected in two live poultry markets and farms 970 etiology samples were, and detected 20 H7N9 avian influenza HIV-positive.

Gene sequence analysis, found that the H7N9 avian influenza gene fragments with H7N9 human isolates highly homologous to people infected with the H7N9 virus, the H7N9 virus may be derived from poultry, the findings published in the "Science Bulletin".

The study also pointed out that the experience with the virus samples from live poultry markets, all farms in the sample were negative, the H7N9 virus may be generated in the live bird markets. Reference laboratories are racing to develop a vaccine specifically for the H7N9 avian influenza virus.
Mainland yesterday of new confirmed a case of Anhui, Zhejiang 2. Patients with 91-year-old man surnamed Wang, Anhui Chuzhou Tianchang City, on the 14th of this month, the disease is now in hospital for treatment. Zhejiang patients are named Hung 84-year-old male, Hangzhou Ji retired workers; 62-year-old male, Hangzhou migrant, the two incidence on the 15th of this month, and was seriously ill.


zhttp://news.sina.com.hk/news/20130424/-2-2951793/1.html
 
Re: China - H7N9 found in chickens says Harbin Veterinary Research Institute: wet markets and farms

what a surprising headline ;-)

20 from 200 samples positive now.
Earlier it was 39 from 40000.

> but all Ducks samples of thirty, failed to reveal the H7N9 virus.

so presumably neither in mallards or other wild waterfowl either

> live poultry market in Shanghai is not difficult to find the virus,
 
Re: China - H7N9 found in chickens says Harbin Veterinary Research Institute: wet markets and farms

> how do the chickens in the wet markets acquire H7N9 if not from the farms from where they came?

I think they distinguish bigger "farms" (--> flozen,supermarkets ?)
and small backyard,family (pet-) chicken growing and selling and exchange

> the epidemic is very will soon be going downhill

> collected in two live poultry markets and farms 970 etiology samples were, and detected 20 H7N9
> avian influenza HIV-positive.

HIV ? maybe human influenza virus in that translation, not human immunodeficiency virus
 
Re: China - H7N9 found in chickens says Harbin Veterinary Research Institute: wet markets and farms

> how do the chickens in the wet markets acquire H7N9 if not from the farms from where they came?

I think they distinguish bigger "farms" (--> flozen,supermarkets ?)
and small backyard,family (pet-) chicken growing and selling and exchange

> the epidemic is very will soon be going downhill

> collected in two live poultry markets and farms 970 etiology samples were, and detected 20 H7N9
> avian influenza HIV-positive.

HIV ? maybe human influenza virus in that translation, not human immunodeficiency virus

There is no mention of homes, backyards, streets, neighborhoods, etc. in either post. I think the reference is to commercial growers. They are protecting the commercial poultry industry.

They are claiming that the source of the human infections is a mystery wet market phenomena.
 
Re: China - H7N9 found in chickens says Harbin Veterinary Research Institute: wet markets and farms

Source: http://news.ifeng.com/mainland/special/h7n9/content-3/detail_2013_04/24/24612644_0.shtml

Ministry of Agriculture of 43 H7N9 avian influenza has been detected positive samples

2013 04 24, 19:31
Source: China News Network

Original title: Ministry of Agriculture has detected 43 positive samples of the H7N9 avian influenza

BEIJING, April 24 message, according to the website of the Ministry of Agriculture, as of April 24, the National Avian Influenza Reference Laboratory detected a total of 43 H7N9 avian influenza positive samples.

Detected in 553 samples collected by the National Avian Influenza Reference Laboratory report in Henan environmental samples were positive for H7N9 avian influenza, from the in the Zhoukou City Chuanhui Jianshe Road farmers market and Kaifeng Weishi County Xiao Taiwan farmers market. Zhejiang animal disease prevention and control center for inspection suspected positive samples for review by the National Avian Influenza Reference Laboratory, were positive for H7N9 avian influenza, chicken samples were collected from the vegetable market, Yiwu, Jinhua, Zhejiang, Chian. Gene sequence analysis showed that all isolates of the H7N9 avian influenza virus strains isolated from pigeons and April 4 highly homologous.

Ministry of Agriculture has asked the Henan Province and Zhejiang Province in strict accordance with the Ministry of Agriculture, Animal H7N9 avian influenza emergency response Guide (Trial) "do a good job related to disposal. At the same time, called for further strengthening of the H7N9 avian influenza virus monitoring the effective implementation of prevention and control measures.
[Editor: PN039] Tags: H7N9 avian influenza
 
Re: China - H7N9 found in chickens says Harbin Veterinary Research Institute: wet markets and farms

Source: http://news.sina.com.cn/c/2013-04-24/195126937225.shtml

The 3 H7N9 avian influenza was detected in the sample of Henan and Zhejiang positive sample
2013 04 24 19:51

China Exclusive: Ministry of Agriculture to 3 copies of H7N9 avian influenza positive samples detected in samples from Henan and Zhejiang Department of Agriculture on the 24th, the samples collected by the National Avian Influenza Reference Laboratory report in Henan detected H7N9 avian influenza positive, respectively, from the Zhoukou City Chuanhui Jianshe Road farmers market Kaifeng Weishi County Xiao Taiwan farmers market. The positive samples of Zhejiang Province, Yiwu, Jinhua, Zhejiang, Chian vegetable market from the chicken.
 
Re: China - H7N9 found in chickens says Harbin Veterinary Research Institute: wet markets to farms

Re: China - H7N9 found in chickens says Harbin Veterinary Research Institute: wet markets to farms

.......... So how do the chickens in the wet markets acquire H7N9 if not from the farms from where they came?

From the wet market vendors???

.
 
Re: China - H7N9 found in chickens says Harbin Veterinary Research Institute: wet markets and farms

Pigeons, cats, dogs and bramblings are all possibilities. Not to mention rats and mice as possible vectors. This virus seems to be able to bounce among species like a ping pong ball.
 
Re: China - H7N9 found in chickens says Harbin Veterinary Research Institute: wet markets and farms

they had H9N2 in several variants since >10-15 years
 
Re: China - H7N9 found in chickens says Harbin Veterinary Research Institute: wet markets and farms

from the eggs ?
 
Re: China - H7N9 found in chickens says Harbin Veterinary Research Institute: wet markets and farms

Ministry of Agriculture Zhejiang submission samples detected 3 copies of H7N9 avian influenza positive samples
Date :2013-04-26 14:09 Author: Source: Ministry of Agriculture Information Office

  NEEDHAM, Massachusetts, the Ministry of Agriculture Information Office released on April 26, Zhejiang Province, animal disease prevention and control center detected in chickens suspected positive samples for review by the National Avian Influenza Reference Laboratory confirmed, 3 were positive for H7N9 avian influenza samples from Zhejiang Province, Lin'an, Hangzhou operating point, the highly homologous isolates April 4, the H7N9 avian influenza virus strains isolated from pigeons.

  Ministry of Agriculture has asked the Zhejiang Province, according to the Ministry of Agriculture, Animal H7N9 avian influenza emergency response guide (Trial) "strictly do disposal. At the same time, called for further strengthening of the H7N9 avian influenza virus monitoring the implementation of prevention and control measures.

  Up to now, the National Avian Influenza Reference Laboratory detected a total of 46 H7N9 avian influenza positive samples.
http://www.moa.gov.cn/zwllm/zwdt/201304/t20130426_3445370.htm
 
Re: China - H7N9 found in chickens says Harbin Veterinary Research Institute: wet markets and farms

Agreed with comments above.

If the farms from whence the chickens (and presumably other poultry) came do not have infection, then the poultry must be getting infected at the wet markets themselves.

So how are they getting infected?

We know it has been detected in pidgeons around wet markets, but was that pidgeons for sale at wet markets or wild ones?

I dont think any of the reports have made that clear?

It would be good to see a report that vermin, cats, dogs etc have been tested in known outbreak areas with infected poultry and found clear. So far, there has been nothing on this.
 
Re: China - H7N9 found in chickens says Harbin Veterinary Research Institute: wet markets and farms

someone should sequence the motor-bikes with the baskets
where they put the chickens into
 
Re: China - H7N9 found in chickens says Harbin Veterinary Research Institute: wet markets and farms

Translation Google

Ministry of Agriculture: a total of 46 positive samples of avian influenza found no pigs infected

Published :2013-4-27 10:02:00
  
BEIJING, April 26 message, according to the website of the Ministry of Agriculture, as of April 26, the provinces and the National Avian Influenza Reference Laboratory were found 46 positive H7N9 avian influenza virus, not isolated H7N9 avian influenza from poultry farms around virus, also found no pigs infected with the virus.
  
Ministry of Agriculture said that as of April 26, the provinces and the National Avian Influenza Reference Laboratory samples 390628, the sample covers 13,014 monitoring sampling field point, of which 2,587 live poultry market, 337 poultry slaughterhouse, 8808 poultry farm (village), 341 wild birds habitat, 277 pig slaughterhouse, 31 pig farms and 633 environmental sampling point.
  
Present, were detected in samples of 218,897 copies, which serological 150,837 samples, 68060 etiology samples. 68060 etiology samples found that 46 H7N9 avian influenza virus, the positive rate of 0.07%. 44 of 14 from Shanghai, Anhui, Zhejiang, Jiangsu and Henan provinces (municipalities) of live poultry business market (points) poultry and environmental samples; 2 positive samples from pigeons, Nanjing, Jiangsu Province samples from MSC County, Nantong City, Jiangsu Province, Pigeon farmers. The remaining samples were negative. Up to now, all over is not isolated from poultry farms to the H7N9 avian influenza virus, also found no pigs infected with the virus.
  
Next, the Ministry of Agriculture will continue to strengthen the sector, in close collaboration to strengthen the linkage. The same time, to further strengthen the monitoring of H7N9 avian influenza virus, strengthen the study of the characteristics of the virus and dissemination of law, to carry out risk assessment and situation judged, effective and orderly and effective good response.

(China News Network)

http://www.99sj.com/News/256597.htm
 
Last edited by a moderator:
Re: China - H7N9 found in chickens says Harbin Veterinary Research Institute: wet markets and farms

Source: http://news.dayoo.com/china/57400/201304/29/57400_110021628.htm

Zhejiang Yiwu market chicken samples the H7N9 positive for the city to stop live animal
http://www.dayoo.com/ 2013-04-29 19:45 Source: Zhejiang Online Reviews (0)

Two nights ago, the Ministry of Agriculture Information Office published a news release bar on its official website epidemic: the Ministry of Agriculture to 3 copies of H7N9 avian influenza positive samples detected from samples of Henan and Zhejiang.

The message: Zhejiang animal disease prevention and control center for inspection suspected positive samples for review by the National Avian Influenza Reference Laboratory, were positive for H7N9 avian influenza, samples were collected from the vegetable market, Yiwu, Jinhua, Zhejiang, Chian chicken.

Visit Chi'an farms

Sampling the the chicken samples H7N9 avian influenza-positive vegetable market, opposite the town of Yiwu Chian benefits of hotel, the locals like to call it the "village vegetable market.


Yesterday afternoon, coming and going, the market is no different with the past.

Fish Zhang, Jiangxi, a few months ago, first came to the vegetable market. For avian influenza things, he was not quite "cold." "At first pass, quite evil, and now, you see, I am in the markets every day, the body is very good."

Zhang opposite the fish stalls, separated by 3 meters, is the problem chicken stalls has been plastic film around it. Through the cracks, I saw inside stacked eight cages, the booth one side there is a stove is used to boil water for the chicken ham hair. Stalls outside the ground, but also keep a lot of the disinfection white powder.

April 16, initially detected the chicken samples H7N9 avian influenza positive, chicken stalls closed.


In the vegetable market, I found a stall she had got, "April 15, someone sample testing results the next day, people come to me about the chicken away. Those days no business almost 10 chickens, eight were taken away. "(Ms. Zhou's memory may deviate According to the records of the relevant departments, the day a total of 12 chickens, 10 of which were taken away processing.)

Zhou said, later also to do a physical examination. "I heard them say, no problem, we can rest assured."

The past few days, the town hospitals doctors every day to the market. Morning Afternoon of all time, to all business operators body temperature, until now, did not find anything. "

Chen Big Brother come to buy vegetables market every day to do the disinfection work, coupled with the chickens, ducks and other poultry is no longer sold, they come here to buy food, do not worry about infection.

It is understood that, as the H7N9 avian flu was first detected positive town in Yiwu, Chian first suspended all poultry trading activities.


Yiwu CDC:

Found no human infection with the H7N9 avian influenza

Yesterday, I went to the the Yiwu CDC.

Yiwu has found no human infection with the H7N9 avian influenza? The Yiwu Communicable Disease Prevention chief Dong Xuanjun first talked about this problem.

"Up to now, Yiwu, the city found no human infection with the H7N9 avian influenza." DONG Xuan Jun said that starting in early April, Yiwu CDC weekly collected by Yiwu Central Hospital more than 15 cases of ordinary flu H7N9 avian influenza virus detection, so far found no suspicious circumstances.


The Dong Xuanjun, while meeting the needs in a timely manner to the case of four categories, Yiwu Central Hospital of human infection with the H7N9 avian influenza virus detection. "The recent history of poultry exposure; temperature greater than or equal to 38 degrees Celsius; flu-like symptoms; leukocyte or lower."

"If people have the above four cases, can go to the nearest community health service centers or Yiwu human infection with the H7N9 avian influenza sentinel clinics hospital Yiwu Central Hospital patient treatment of fever, the fever clinic doctor according to your actual situation to determine need to detect from April 12, has been on the medical staff of the city's medical and health units and private out-patient units in batches training. "

For the consumption of poultry products, DONG Xuan Army gives his own views. 65 degrees 30 minutes inactivated H7N9 avian influenza virus in the environment of 100 degrees Celsius, as long as two minutes can be inactivated, therefore, is not to say that poultry products can not eat, but special attention, Recently consumption poultry food to be cooked fully cooked. "

Livestock sector: Pause live animal

April 23, Yiwu municipal government issued an emergency notice requirements towns Street and the relevant government departments to immediately implement the measures: suspension of live poultry trading; further expand the scope of monitoring; suspension of live poultry transported; carry out illegal feeding of birds culled .

Yesterday, Yiwu Husbandry and Veterinary Bureau staff said: they have strict accordance with the Ministry of Agriculture, Animal H7N9 avian influenza emergency response Guide (Trial) "requirement the Chian vegetable market and remain live chickens sound processing, and venues strict disinfection, a citywide dragnet investigation of the outbreak and epidemiological investigation, starting from April 14, sampling a total of 1200 samples, except Chian, all testing qualified.

Slaughterhouses, markets, farms, three groups of sampling and monitoring work, the moment did not stop, April 23, the city stop live animal, we go to the farms, supervision, is still illegal sales immediately harmless treatment, disinfection work did not do a good job in a timely manner, we immediately notify the relevant departments, to protect the security market. "

H7N9 avian flu suddenly to the livestock industry in Yiwu was injured. In the prevention and control of avian infection at the same time, in order not to let the whole industry collapse, according to the relevant policies in the province, Yiwu livestock sector also strengthened the industry support.

Avian field, each 15 yuan subsidy, the leading enterprises acquired a chicken subsidy, other farms according to size, has also been given compensation policies. "Chen Binfeng
 
Re: China - H7N9 found in chickens says Harbin Veterinary Research Institute: wet markets and farms

Agreed with comments above.

If the farms from whence the chickens (and presumably other poultry) came do not have infection, then the poultry must be getting infected at the wet markets themselves.

So how are they getting infected?...........

From infected (including asymptomatic) vendors?

.
 
Re: China - H7N9 found in chickens says Harbin Veterinary Research Institute: wet markets and farms

From infected (including asymptomatic) vendors?

.

Unlocking
a
3 Piece 4D Puzzle​

Existence of Keys to Emergent H7N9

A sub-clinical disease pattern in most host subjects of emergent H7N9 with low-to-no morbidity allows "silent spread" between the 3 vector classes with the various mammal species being the most important vector at this time. The genetic evidence presented on this thread and these forums should guide lab heads to task principal investigators with re-examining conventional conclusions about sub-segment viral interchange between terrestrial birds (primarily commercial poultry), mammals (people, canines, felines, mink, seals and whales) and roving birds (anseriformes and passerine).

Historical Method of Reservoir Identification

The emergent H7N9 dynamic may appear unusual in the area of pinpointing a reservoir, but is, in fact, no more unusual than the emergent 2009 H1N1 pandemic where no valid cornerstone reservoir was ever defined, though the public has been consistently led to believe the disease originated in swine. Effort has been expended at every level of virology, extensive effort, to validate the conventional science assumption of swine as the 2009 pandemic base.

More than 7,000 H1 swine sequences appear in the database today with 5,800 after 2004 and 4,900 since 2008. More swine sequences are on file for H1 serotypes than all H5 sequences of all species combined and 3 times the number of all H7 sequences for all species combined.

This costly pursuit of their assumption has not returned a positive finding, but has been prosecuted to adverse effect for surveillance on more probable subjects. The idea that science can conclude so rapidly on a complex problem with diminishing support data and with readily available confounding information defies the very concept of science, of knowledge discovery. This vehement adherence to traditional conclusions, nothing more than blind faith in practice, has no place in disease investigation.

Footing for H7N9 Emergence

The world entered this emergency with a toolkit built around the vast observational database for H7N9 of 26 sequences with analytic-limiting geographic and temporal dispersion (only 6 with 8 complete segments). Today, 4 weeks after announcement of a potential pandemic and 8 weeks after confirmed cases, a total of 15 H7N9 genomes are on file with 8 complete segments (47 HA, 36 HA+NA) and 2 of those are unacknowledged and unannotated practical duplicates with conflicting metadata.

Understanding the disease and preventing potential spread to 1.6 billion people (25% CAR) hinges on a count of pathogen specimens produced by the stellar international virology labs loitering at a population slightly higher than a baker's dozen box of doughnuts.

That's neither good eating, nor sufficient fuel for analysis. Confidence is not generally inspired by those striking so limited a foundation. Perhaps more remains to be understood concerning the initiation, development and maturation of pandemic virus reservoirs. And then again, perhaps the data is of no import to this matter:

Dys-Contiguous emergence in Hunan province was predicted on the 14th of April using the aforementioned limited genetic database. Yesterday, emergence was verified in Hunan as early as April 14th on a positive test confirmation.

System Dynamics of Vector Classes

Complex interplay exists between the three vector classes. Bi-directional transmission will eventually be understood at each level using the proper necropsy procedures and with revision of human testing sensitivity.

  • Poultry incubates (chicken, duck with emphases on quail, chukkar, partridge & ratite)
  • People incubate (male /female, both tails of the age-to-population curve: very young and very old)
  • Passerines incubate and transport over distance (magpies, crows, jays, martins, swallows with emphases on pets & pigeons).

Emergent H7N9 with the proven movement of genetic data involving common passerines may appear daunting and fall outside standing models now, but the ΣH7N9 may be found at some point in the future to be using a very normal form of step-wise genetic acquisition for a pandemic goal-seeking organism. But we may never know because new models arise from data and H7N9 data is sparse geographically, sparse temporally and austerely sparse in every area but sparsity.

Exchange Proposal: Keys For Data

We'd like to propose an exchange: keys for data.​

Participants are invited to explore anywhere you want with whatever vehicle you can start with these keys (Open Access, Full Text). Just report back what you find on your trek (GISAID with any ethical clinicals in any language). We've asked for specific data on many previous occasions and have offered the keys as well, but we'll give it another try:​

Passerines: Pets & Pigeons
3P's of the emergent H7N9 Puzzle
These 3P's are not dissimilar to 3 keys.​


Summary of RFI

One puzzle, four dimensions, three keys . . . all that's missing is one bright lab head. Who will invest the fourth dimension, take these keys, wrinkle the fabric and approach the puzzle?
 
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