tetano
Editor, Senior Moderator
Clin Infect Dis
. 2022 Feb 18;ciac142.
doi: 10.1093/cid/ciac142. Online ahead of print.
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections by intranasal or testicular inoculation induces testicular damage preventable by vaccination in golden Syrian hamsters
Can Li[SUP] 1 2 [/SUP], Zhanhong Ye[SUP] 1 [/SUP], Anna Jin-Xia Zhang[SUP] 1 2 3 [/SUP], Jasper Fuk-Woo Chan[SUP] 1 2 3 4 5 6 [/SUP], Wenchen Song[SUP] 1 2 [/SUP], Feifei Liu[SUP] 1 [/SUP], Yanxia Chen[SUP] 1 [/SUP], Mike Yat-Wah Kwan[SUP] 7 [/SUP], Andrew Chak-Yiu Lee[SUP] 1 2 [/SUP], Yan Zhao[SUP] 1 [/SUP], Bosco Ho-Yin Wong[SUP] 1 2 [/SUP], Cyril Chik-Yan Yip[SUP] 4 [/SUP], Jian-Piao Cai[SUP] 1 [/SUP], David Christopher Lung[SUP] 8 [/SUP], Siddharth Sridhar[SUP] 1 2 3 4 [/SUP], Dongyan Jin[SUP] 6 9 [/SUP], Hin Chu[SUP] 1 2 3 [/SUP], Kelvin Kai-Wang To[SUP] 1 2 3 4 [/SUP], Kwok-Yung Yuen[SUP] 1 2 3 4 5 6 [/SUP]
Affiliations
Abstract
Background: The role of SARS-CoV-2 in the pathogenesis of testicular damage is uncertain.
Methods: We investigated the virological, pathological, and immunological changes in testes of hamsters challenged by SARS-CoV-2 wild-type and its variants by intranasal or direct testicular inoculation using influenza virus A(H1N1)pdm09 as control.
Results: Besides self-limiting respiratory tract infection, intranasal SARS-CoV-2 challenge caused acute decrease in sperm count, and serum testosterone and inhibin B at 4 to 7 days post-infection (dpi), and subsequently reduced testicular size and weight, and serum sex hormone level at 42 to 120 dpi. Acute histopathological damage with varying degree of testicular inflammation, haemorrhage, and necrosis, degeneration of seminiferous tubules and disruption of orderly spermatogenesis were seen with increasing virus inoculum. Degeneration and necrosis of Sertoli and Leydig cells were found. Though viral loads and SARS-CoV-2 nucleocapid (N) protein expression were markedly lower in testicular than lung tissues, direct intra-testicular injection showed N expressing interstitial cells and epididymal epithelial cells. Control intranasal or intra-testicular challenge by A(H1N1)pdm09 showed no testicular infection or damage. From 7 to 120 dpi, degeneration and apoptosis of seminiferous tubules, immune complex deposition and depletion of spermatogenic cell and spermatozoa persisted. Intranasal challenge with Omicron and Delta variants could also induce similar testicular changes. These testicular damages can be prevented by vaccination.
Conclusions: SARS-CoV-2 can cause acute testicular damage with subsequent chronic asymmetric testicular atrophy and associated hormonal changes despite a self-limiting pneumonia in hamsters. Awareness of possible hypogonadism and subfertility is important in managing convalescent COVID-19 males.
Keywords: COVID-19; SARS-CoV-2; coronavirus; hamster; testicle.
. 2022 Feb 18;ciac142.
doi: 10.1093/cid/ciac142. Online ahead of print.
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections by intranasal or testicular inoculation induces testicular damage preventable by vaccination in golden Syrian hamsters
Can Li[SUP] 1 2 [/SUP], Zhanhong Ye[SUP] 1 [/SUP], Anna Jin-Xia Zhang[SUP] 1 2 3 [/SUP], Jasper Fuk-Woo Chan[SUP] 1 2 3 4 5 6 [/SUP], Wenchen Song[SUP] 1 2 [/SUP], Feifei Liu[SUP] 1 [/SUP], Yanxia Chen[SUP] 1 [/SUP], Mike Yat-Wah Kwan[SUP] 7 [/SUP], Andrew Chak-Yiu Lee[SUP] 1 2 [/SUP], Yan Zhao[SUP] 1 [/SUP], Bosco Ho-Yin Wong[SUP] 1 2 [/SUP], Cyril Chik-Yan Yip[SUP] 4 [/SUP], Jian-Piao Cai[SUP] 1 [/SUP], David Christopher Lung[SUP] 8 [/SUP], Siddharth Sridhar[SUP] 1 2 3 4 [/SUP], Dongyan Jin[SUP] 6 9 [/SUP], Hin Chu[SUP] 1 2 3 [/SUP], Kelvin Kai-Wang To[SUP] 1 2 3 4 [/SUP], Kwok-Yung Yuen[SUP] 1 2 3 4 5 6 [/SUP]
Affiliations
- PMID: 35178548
- DOI: 10.1093/cid/ciac142
Abstract
Background: The role of SARS-CoV-2 in the pathogenesis of testicular damage is uncertain.
Methods: We investigated the virological, pathological, and immunological changes in testes of hamsters challenged by SARS-CoV-2 wild-type and its variants by intranasal or direct testicular inoculation using influenza virus A(H1N1)pdm09 as control.
Results: Besides self-limiting respiratory tract infection, intranasal SARS-CoV-2 challenge caused acute decrease in sperm count, and serum testosterone and inhibin B at 4 to 7 days post-infection (dpi), and subsequently reduced testicular size and weight, and serum sex hormone level at 42 to 120 dpi. Acute histopathological damage with varying degree of testicular inflammation, haemorrhage, and necrosis, degeneration of seminiferous tubules and disruption of orderly spermatogenesis were seen with increasing virus inoculum. Degeneration and necrosis of Sertoli and Leydig cells were found. Though viral loads and SARS-CoV-2 nucleocapid (N) protein expression were markedly lower in testicular than lung tissues, direct intra-testicular injection showed N expressing interstitial cells and epididymal epithelial cells. Control intranasal or intra-testicular challenge by A(H1N1)pdm09 showed no testicular infection or damage. From 7 to 120 dpi, degeneration and apoptosis of seminiferous tubules, immune complex deposition and depletion of spermatogenic cell and spermatozoa persisted. Intranasal challenge with Omicron and Delta variants could also induce similar testicular changes. These testicular damages can be prevented by vaccination.
Conclusions: SARS-CoV-2 can cause acute testicular damage with subsequent chronic asymmetric testicular atrophy and associated hormonal changes despite a self-limiting pneumonia in hamsters. Awareness of possible hypogonadism and subfertility is important in managing convalescent COVID-19 males.
Keywords: COVID-19; SARS-CoV-2; coronavirus; hamster; testicle.