Clin Infect Dis . Ultra-sensitive Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Antigen Detection for the Diagnosis of Coronavirus D

[tetano]

Clin Infect Dis


. 2021 Apr 8;ciab063.
doi: 10.1093/cid/ciab063. Online ahead of print.
Ultra-sensitive Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Antigen Detection for the Diagnosis of Coronavirus Disease 2019 (COVID-19) in Upper Respiratory Samples


Hannah Wang[SUP] 1 [/SUP], Catherine A Hogan[SUP] 1 [/SUP], Michelle Verghese[SUP] 1 [/SUP], Daniel Solis[SUP] 1 [/SUP], Mamdouh Sibai[SUP] 1 [/SUP], ChunHong Huang[SUP] 1 [/SUP], James Zehnder[SUP] 1 [/SUP], Malaya K Sahoo[SUP] 1 [/SUP], Benjamin A Pinsky[SUP] 1 2 [/SUP]



Affiliations

• PMID: 33830203
• DOI: 10.1093/cid/ciab063

Abstract

An ultra-sensitive severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleocapsid antigen assay (S-PLEX, MesoScale Diagnostics) was evaluated in 250 retrospective and 200 prospective upper respiratory specimens. In samples with cycle threshold <35, there was 95%-98% positive and 93%-96% negative percent agreement with reverse transcription-polymerase chain reaction. S-PLEX may provide a high-throughput alternative to nucleic acid-based testing for coronavirus disease 2019 (COVID-19) diagnosis.

Keywords: COVID-19; COVID-19 testing; SARS-COV-2 antigen testing; SARS-CoV-2.