tetano
Editor, Senior Moderator
Clin Lab Med
. 2020 Dec;40(4):459-472.
doi: 10.1016/j.cll.2020.08.004. Epub 2020 Aug 8.
Coronavirus Detection in the Clinical Microbiology Laboratory: Are We Ready for Identifying and Diagnosing a Novel Virus?
Katharine Uhteg[SUP] 1 [/SUP], Karen C Carroll[SUP] 1 [/SUP], Heba H Mostafa[SUP] 2 [/SUP]
Affiliations
Abstract
Endemic species of coronavirus (HCoV-OC43, HCoV-229E, HCoV-NL63, and HCoV-HKU1) are frequent causes of upper respiratory tract infections. Three highly pathogenic coronaviruses have been associated with outbreaks and epidemics and have challenged clinical microbiology laboratories to quickly develop assays for diagnosis. Their initial characterization was achieved by molecular methods. With the great advance in metagenomic whole-genome sequencing directly from clinical specimens, diagnosis of novel coronaviruses could be quickly implemented into the workflow of managing cases of pneumonia of unknown cause, which will markedly affect the time of the initial characterization and accelerate the initiation of outbreak control measures.
Keywords: COVID-19; Coronaviruses; MERS; Molecular diagnosis; SARS; SARS-CoV-2 (2019-nCoV); Sequencing.
. 2020 Dec;40(4):459-472.
doi: 10.1016/j.cll.2020.08.004. Epub 2020 Aug 8.
Coronavirus Detection in the Clinical Microbiology Laboratory: Are We Ready for Identifying and Diagnosing a Novel Virus?
Katharine Uhteg[SUP] 1 [/SUP], Karen C Carroll[SUP] 1 [/SUP], Heba H Mostafa[SUP] 2 [/SUP]
Affiliations
- PMID: 33121615
- DOI: 10.1016/j.cll.2020.08.004
Abstract
Endemic species of coronavirus (HCoV-OC43, HCoV-229E, HCoV-NL63, and HCoV-HKU1) are frequent causes of upper respiratory tract infections. Three highly pathogenic coronaviruses have been associated with outbreaks and epidemics and have challenged clinical microbiology laboratories to quickly develop assays for diagnosis. Their initial characterization was achieved by molecular methods. With the great advance in metagenomic whole-genome sequencing directly from clinical specimens, diagnosis of novel coronaviruses could be quickly implemented into the workflow of managing cases of pneumonia of unknown cause, which will markedly affect the time of the initial characterization and accelerate the initiation of outbreak control measures.
Keywords: COVID-19; Coronaviruses; MERS; Molecular diagnosis; SARS; SARS-CoV-2 (2019-nCoV); Sequencing.