tetano
Editor, Senior Moderator
Clin Respir J
. 2021 Jan 23.
doi: 10.1111/crj.13331. Online ahead of print.
Development of SARS-CoV-2 Rapid Antibody Detection Kit and Study on Dynamic Changes in Antibodies in Infected Patients
Huihui Wang[SUP] 1 [/SUP], Xuemei Li[SUP] 1 [/SUP], Tao Li[SUP] 1 2 [/SUP], Lianzi Wang[SUP] 1 [/SUP], Li Wang[SUP] 3 [/SUP], Jiawang Lin[SUP] 3 [/SUP], Shubing Zhang[SUP] 1 [/SUP], Yuanhong Xu[SUP] 1 [/SUP], Wei Wei[SUP] 2 [/SUP]
Affiliations
Abstract
Introduction: Severe acute respiratory syndrome coronavirus 2 (SARS-COV-2) has spread rapidly to 185 regions and countries around the world with more than 2.8 million confirmed infections and 203,044 deaths. Respiratory diseases caused by SARS-COV-2 are serious threats to human health.
Objectives: To develop a rapid detection kit for new coronavirus antibodies and use it to study the dynamic changes in antibodies in clinically confirmed SARS-CoV-2 infected patients.
Methods: The SARS-CoV-2 IgM/IgG antibody test kit (Colloidal Gold Method) was developed. Serum SARS-CoV-2 IgM and IgG antibodies were tested in SARS-CoV-2 and non-SARS-CoV-2 infected persons, respectively.
Results and conclusion: The sensitivities of the SARS-CoV-2 IgM/IgG antibody test kit (Colloidal Gold Method) were 50%, 70%, 92.5%, and 97.5% after 1-3 days, 4-6 days, 7-9 days, and > 9 days after admission, and the specificities of the IgM, IgG, and IgM + IgG antibodies were all 100%. Using the SARS-CoV-2 IgM/IgG antibody test kit (Colloidal Gold Method), the positive rates of SARS-CoV-2 IgM and IgG antibodies increased from 50% to 92.5% after 1-3 days, 4-6 days, and 7-9 days of admission, which showed an increasing trend. The titers of the SARS-CoV-2 IgM and IgG antibodies in the positive specimens increased with the length of admission.
Keywords: COVID-19; SARS-CoV-2; antibody; colloidal gold immunochromatography; infection.
. 2021 Jan 23.
doi: 10.1111/crj.13331. Online ahead of print.
Development of SARS-CoV-2 Rapid Antibody Detection Kit and Study on Dynamic Changes in Antibodies in Infected Patients
Huihui Wang[SUP] 1 [/SUP], Xuemei Li[SUP] 1 [/SUP], Tao Li[SUP] 1 2 [/SUP], Lianzi Wang[SUP] 1 [/SUP], Li Wang[SUP] 3 [/SUP], Jiawang Lin[SUP] 3 [/SUP], Shubing Zhang[SUP] 1 [/SUP], Yuanhong Xu[SUP] 1 [/SUP], Wei Wei[SUP] 2 [/SUP]
Affiliations
- PMID: 33484220
- DOI: 10.1111/crj.13331
Abstract
Introduction: Severe acute respiratory syndrome coronavirus 2 (SARS-COV-2) has spread rapidly to 185 regions and countries around the world with more than 2.8 million confirmed infections and 203,044 deaths. Respiratory diseases caused by SARS-COV-2 are serious threats to human health.
Objectives: To develop a rapid detection kit for new coronavirus antibodies and use it to study the dynamic changes in antibodies in clinically confirmed SARS-CoV-2 infected patients.
Methods: The SARS-CoV-2 IgM/IgG antibody test kit (Colloidal Gold Method) was developed. Serum SARS-CoV-2 IgM and IgG antibodies were tested in SARS-CoV-2 and non-SARS-CoV-2 infected persons, respectively.
Results and conclusion: The sensitivities of the SARS-CoV-2 IgM/IgG antibody test kit (Colloidal Gold Method) were 50%, 70%, 92.5%, and 97.5% after 1-3 days, 4-6 days, 7-9 days, and > 9 days after admission, and the specificities of the IgM, IgG, and IgM + IgG antibodies were all 100%. Using the SARS-CoV-2 IgM/IgG antibody test kit (Colloidal Gold Method), the positive rates of SARS-CoV-2 IgM and IgG antibodies increased from 50% to 92.5% after 1-3 days, 4-6 days, and 7-9 days of admission, which showed an increasing trend. The titers of the SARS-CoV-2 IgM and IgG antibodies in the positive specimens increased with the length of admission.
Keywords: COVID-19; SARS-CoV-2; antibody; colloidal gold immunochromatography; infection.