Mary Wilson
Well-known member
Available online 28 February 2023, 105419
https://doi.org/10.1016/j.jcv.2023.105419
Jean-Louis Bayart [SUP]a[/SUP], Constant Gillot [SUP]b[/SUP] [SUP]1[/SUP], Jean-Michel Dogné [SUP]b[/SUP], Gatien Roussel [SUP]a[/SUP], Valérie Verbelen [SUP]a[/SUP], Julien Favresse [SUP]b[/SUP] [SUP]c[/SUP], Jonathan Douxfils [SUP]b[/SUP] [SUP]d[/SUP]
Abstract
Background
A SARS-CoV-2+Flu A/B+RSV Combo Rapid test may be more relevant than Rapid Antigen Diagnostic (RAD) tests targeting only SARS-CoV-2 since we are facing a concurrent circulation of these viruses during the winter season.
Objectives
To assess the clinical performance of a SARS-CoV-2+Flu A/B+RSV Combo test in comparison to a multiplex RT-qPCR.
Study Design
Residual nasopharyngeal swabs issued from 178 patients were included. All patients, adults and children, were symptomatic and presented at the emergency department with flu-like symptoms. Characterization of the infectious viral agent was done by RT-qPCR. The viral load was expressed as cycle threshold (Ct). Samples were then tested using the multiplex RAD test Fluorecare[SUP]®ฏ[/SUP] SARS-CoV-2 & Influenza A/B & RSV Antigen Combo Test. Data analysis was carried out using descriptive statistics.
Results
The sensitivity of the test varies according to the virus, with the highest sensitivity observed for Influenza A (80.8.% [95%CI: 67.2 - 94.4]) and the lowest sensitivity observed for RSV (41.5% [95%CI: 26.2 - 56.8]). Higher sensitivities were observed for samples with high viral loads (Ct < 20) and decrease with low viral loads. The specificity for SARS-CoV-2, RSV and Influenza A and B was >95%.
Conclusions
The Fluorecare® combo antigenic presents satisfying performance in real-life clinical setting for Influenza A and B in samples with high viral load. This could be useful to allow a rapid (self-)isolation as the transmissibility of these viruses increase with the viral load. According to our results, its use to rule-out SARS-CoV-2 and RSV infection is not sufficient.
https://www.sciencedirect.com/science/article/pii/S1386653223000410?dgcid=rss_sd_all
https://doi.org/10.1016/j.jcv.2023.105419
Jean-Louis Bayart [SUP]a[/SUP], Constant Gillot [SUP]b[/SUP] [SUP]1[/SUP], Jean-Michel Dogné [SUP]b[/SUP], Gatien Roussel [SUP]a[/SUP], Valérie Verbelen [SUP]a[/SUP], Julien Favresse [SUP]b[/SUP] [SUP]c[/SUP], Jonathan Douxfils [SUP]b[/SUP] [SUP]d[/SUP]
Abstract
Background
A SARS-CoV-2+Flu A/B+RSV Combo Rapid test may be more relevant than Rapid Antigen Diagnostic (RAD) tests targeting only SARS-CoV-2 since we are facing a concurrent circulation of these viruses during the winter season.
Objectives
To assess the clinical performance of a SARS-CoV-2+Flu A/B+RSV Combo test in comparison to a multiplex RT-qPCR.
Study Design
Residual nasopharyngeal swabs issued from 178 patients were included. All patients, adults and children, were symptomatic and presented at the emergency department with flu-like symptoms. Characterization of the infectious viral agent was done by RT-qPCR. The viral load was expressed as cycle threshold (Ct). Samples were then tested using the multiplex RAD test Fluorecare[SUP]®ฏ[/SUP] SARS-CoV-2 & Influenza A/B & RSV Antigen Combo Test. Data analysis was carried out using descriptive statistics.
Results
The sensitivity of the test varies according to the virus, with the highest sensitivity observed for Influenza A (80.8.% [95%CI: 67.2 - 94.4]) and the lowest sensitivity observed for RSV (41.5% [95%CI: 26.2 - 56.8]). Higher sensitivities were observed for samples with high viral loads (Ct < 20) and decrease with low viral loads. The specificity for SARS-CoV-2, RSV and Influenza A and B was >95%.
Conclusions
The Fluorecare® combo antigenic presents satisfying performance in real-life clinical setting for Influenza A and B in samples with high viral load. This could be useful to allow a rapid (self-)isolation as the transmissibility of these viruses increase with the viral load. According to our results, its use to rule-out SARS-CoV-2 and RSV infection is not sufficient.
https://www.sciencedirect.com/science/article/pii/S1386653223000410?dgcid=rss_sd_all