tetano
Editor, Senior Moderator
Antiviral Res. 2015 Aug 4. pii: S0166-3542(15)00174-6. doi: 10.1016/j.antiviral.2015.08.001. [Epub ahead of print]
[h=1]Co-immunization with tandem repeat heterologous M2 extracellular proteins overcomes strain-specific protection of split vaccine against influenza A virus.[/h] Lee YN[SUP]1[/SUP], Kim MC[SUP]2[/SUP], Lee YT[SUP]1[/SUP], Kim YJ[SUP]1[/SUP], Lee J[SUP]3[/SUP], Kim C[SUP]3[/SUP], Ha SH[SUP]4[/SUP], Kang SM[SUP]5[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Current influenza vaccines are less efficacious against antigenically different influenza A viruses. This study presents an approach to overcome strain-specific protection, using a strategy of co-immunization with seasonal H3N2 split vaccine and yeast-expressed soluble proteins of a tandem repeat containing heterologous influenza M2 ectodomains (M2e5x). Co-immunization with both vaccines in mice was superior to either vaccine alone in inducing cross protection against heterologous H3N2 virus by raising M2e-specific humoral and cellular immune responses toward a T-helper type 1 profile inducing IgG2a isotype antibodies as well as interferon-γ-producing cells in systemic and mucosal sites. In addition, co-immunization sera were found to confer cross-protection against different subtypes of H1N1 and H5N1 influenza A viruses in na?ve mice. A mechanistic study provides evidence that activation of dendritic cells by co-stimulation with M2e5x and split vaccine was associated with the proliferation of CD4[SUP]+[/SUP] T cells. Our results suggest that a strategy of co-immunization with seasonal split and M2e5x protein vaccines could be a promising approach for overcoming the limitation of strain-specific protection by current influenza vaccination.
Copyright ? 2015. Published by Elsevier B.V.
[h=4]KEYWORDS:[/h] AS04; Influenza virus; M2e5x; Protein; Split vaccine
PMID: 26248203 [PubMed - as supplied by publisher]
[h=1]Co-immunization with tandem repeat heterologous M2 extracellular proteins overcomes strain-specific protection of split vaccine against influenza A virus.[/h] Lee YN[SUP]1[/SUP], Kim MC[SUP]2[/SUP], Lee YT[SUP]1[/SUP], Kim YJ[SUP]1[/SUP], Lee J[SUP]3[/SUP], Kim C[SUP]3[/SUP], Ha SH[SUP]4[/SUP], Kang SM[SUP]5[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Current influenza vaccines are less efficacious against antigenically different influenza A viruses. This study presents an approach to overcome strain-specific protection, using a strategy of co-immunization with seasonal H3N2 split vaccine and yeast-expressed soluble proteins of a tandem repeat containing heterologous influenza M2 ectodomains (M2e5x). Co-immunization with both vaccines in mice was superior to either vaccine alone in inducing cross protection against heterologous H3N2 virus by raising M2e-specific humoral and cellular immune responses toward a T-helper type 1 profile inducing IgG2a isotype antibodies as well as interferon-γ-producing cells in systemic and mucosal sites. In addition, co-immunization sera were found to confer cross-protection against different subtypes of H1N1 and H5N1 influenza A viruses in na?ve mice. A mechanistic study provides evidence that activation of dendritic cells by co-stimulation with M2e5x and split vaccine was associated with the proliferation of CD4[SUP]+[/SUP] T cells. Our results suggest that a strategy of co-immunization with seasonal split and M2e5x protein vaccines could be a promising approach for overcoming the limitation of strain-specific protection by current influenza vaccination.
Copyright ? 2015. Published by Elsevier B.V.
[h=4]KEYWORDS:[/h] AS04; Influenza virus; M2e5x; Protein; Split vaccine
PMID: 26248203 [PubMed - as supplied by publisher]