tetano
Editor, Senior Moderator
Vet Microbiol. 2017 May;203:143-148. doi: 10.1016/j.vetmic.2017.03.004. Epub 2017 Mar 6.
[h=1]Coimmunization with recombinant epitope-expressing baculovirus enhances protective effects of inactivated H5N1 vaccine against heterologous virus.[/h] Zhang Z[SUP]1[/SUP], Zhang J[SUP]1[/SUP], Zhang J[SUP]1[/SUP], Li Q[SUP]1[/SUP], Miao P[SUP]1[/SUP], Liu J[SUP]1[/SUP], Li S[SUP]2[/SUP], Huang J[SUP]2[/SUP], Liao M[SUP]3[/SUP], Fan H[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] H5N1, a highly pathogenic avian influenza virus (HPAIV), poses a significant threat to poultry and human health. However, currently available inactivated influenza vaccines are less efficacious against viruses that display antigenic drift. In this study, we constructed a recombinant baculovirus (BV-HMNN) expressing four conserved antigen epitopes: H5N1 hemagglutinin stem area amino acids 76-130 (HA2 76-130); three tandem repeats from the ectodomain of the conserved influenza matrix protein M2 (3M2e); nucleoprotein amino acids 55-69 (NP55-69); and nucleoprotein amino acids 380-393 (NP380-393). We evaluated the immunogenicity and protective efficacy of coimmunization with an inactivated avian influenza virus vaccine (Re6) and the recombinant baculovirus (BV-HMNN) against heterologous viral infection in specific-pathogen-free chickens. The chickens immunized with both vaccines (Re6+BV-HMNN) achieved complete protection, was significantly greater than that of chickens vaccinated with Re6 alone. BV-HMNN-supplemented vaccination also reduced viral shedding more effectively than nonsupplemented vaccination. We conclude that coimmunization with both vaccines was superior to immunization with the inactivated vaccine alone in inducing cross-protection against heterologous H5N1 virus.
Copyright ? 2017. Published by Elsevier B.V.
[h=4]KEYWORDS:[/h] Avian influenza virus; Baculovirus; Epitope; H5N1; Inactivated vaccine
PMID: 28619136 DOI: 10.1016/j.vetmic.2017.03.004
[h=1]Coimmunization with recombinant epitope-expressing baculovirus enhances protective effects of inactivated H5N1 vaccine against heterologous virus.[/h] Zhang Z[SUP]1[/SUP], Zhang J[SUP]1[/SUP], Zhang J[SUP]1[/SUP], Li Q[SUP]1[/SUP], Miao P[SUP]1[/SUP], Liu J[SUP]1[/SUP], Li S[SUP]2[/SUP], Huang J[SUP]2[/SUP], Liao M[SUP]3[/SUP], Fan H[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] H5N1, a highly pathogenic avian influenza virus (HPAIV), poses a significant threat to poultry and human health. However, currently available inactivated influenza vaccines are less efficacious against viruses that display antigenic drift. In this study, we constructed a recombinant baculovirus (BV-HMNN) expressing four conserved antigen epitopes: H5N1 hemagglutinin stem area amino acids 76-130 (HA2 76-130); three tandem repeats from the ectodomain of the conserved influenza matrix protein M2 (3M2e); nucleoprotein amino acids 55-69 (NP55-69); and nucleoprotein amino acids 380-393 (NP380-393). We evaluated the immunogenicity and protective efficacy of coimmunization with an inactivated avian influenza virus vaccine (Re6) and the recombinant baculovirus (BV-HMNN) against heterologous viral infection in specific-pathogen-free chickens. The chickens immunized with both vaccines (Re6+BV-HMNN) achieved complete protection, was significantly greater than that of chickens vaccinated with Re6 alone. BV-HMNN-supplemented vaccination also reduced viral shedding more effectively than nonsupplemented vaccination. We conclude that coimmunization with both vaccines was superior to immunization with the inactivated vaccine alone in inducing cross-protection against heterologous H5N1 virus.
Copyright ? 2017. Published by Elsevier B.V.
[h=4]KEYWORDS:[/h] Avian influenza virus; Baculovirus; Epitope; H5N1; Inactivated vaccine
PMID: 28619136 DOI: 10.1016/j.vetmic.2017.03.004