tetano
Editor, Senior Moderator
Colloid Interface Sci
. 2021 Jun 12;602:732-739.
doi: 10.1016/j.jcis.2021.06.056. Online ahead of print.
SARS-CoV-2 spike protein removes lipids from model membranes and interferes with the capacity of high density lipoprotein to exchange lipids
Yubexi Correa[SUP] 1 [/SUP], Sarah Waldie[SUP] 2 [/SUP], Michel Thépaut[SUP] 3 [/SUP], Samantha Micciula[SUP] 4 [/SUP], Martine Moulin[SUP] 5 [/SUP], Franck Fieschi[SUP] 6 [/SUP], Harald Pichler[SUP] 7 [/SUP], V Trevor Forsyth[SUP] 8 [/SUP], Michael Haertlein[SUP] 9 [/SUP], Marité Cárdenas[SUP] 10 [/SUP]
Affiliations
Abstract
Cholesterol has been shown to affect the extent of coronavirus binding and fusion to cellular membranes. The severity of Covid-19 infection is also known to be correlated with lipid disorders. Furthermore, the levels of both serum cholesterol and high-density lipoprotein (HDL) decrease with Covid-19 severity, with normal levels resuming once the infection has passed. Here we demonstrate that the SARS-CoV-2 spike (S) protein interferes with the function of lipoproteins, and that this is dependent on cholesterol. In particular, the ability of HDL to exchange lipids from model cellular membranes is altered when co-incubated with the spike protein. Additionally, the S protein removes lipids and cholesterol from model membranes. We propose that the S protein affects HDL function by removing lipids from it and remodelling its composition/structure.
Keywords: Cholesterol; Infrared spectroscopy; Lipids; Neutron reflection; SARS-CoV-2 spike protein.
. 2021 Jun 12;602:732-739.
doi: 10.1016/j.jcis.2021.06.056. Online ahead of print.
SARS-CoV-2 spike protein removes lipids from model membranes and interferes with the capacity of high density lipoprotein to exchange lipids
Yubexi Correa[SUP] 1 [/SUP], Sarah Waldie[SUP] 2 [/SUP], Michel Thépaut[SUP] 3 [/SUP], Samantha Micciula[SUP] 4 [/SUP], Martine Moulin[SUP] 5 [/SUP], Franck Fieschi[SUP] 6 [/SUP], Harald Pichler[SUP] 7 [/SUP], V Trevor Forsyth[SUP] 8 [/SUP], Michael Haertlein[SUP] 9 [/SUP], Marité Cárdenas[SUP] 10 [/SUP]
Affiliations
- PMID: 34157514
- DOI: 10.1016/j.jcis.2021.06.056
Abstract
Cholesterol has been shown to affect the extent of coronavirus binding and fusion to cellular membranes. The severity of Covid-19 infection is also known to be correlated with lipid disorders. Furthermore, the levels of both serum cholesterol and high-density lipoprotein (HDL) decrease with Covid-19 severity, with normal levels resuming once the infection has passed. Here we demonstrate that the SARS-CoV-2 spike (S) protein interferes with the function of lipoproteins, and that this is dependent on cholesterol. In particular, the ability of HDL to exchange lipids from model cellular membranes is altered when co-incubated with the spike protein. Additionally, the S protein removes lipids and cholesterol from model membranes. We propose that the S protein affects HDL function by removing lipids from it and remodelling its composition/structure.
Keywords: Cholesterol; Infrared spectroscopy; Lipids; Neutron reflection; SARS-CoV-2 spike protein.