tetano
Editor, Senior Moderator
J Immunol Methods. 2013 Dec 2. pii: S0022-1759(13)00360-8. doi: 10.1016/j.jim.2013.11.023. [Epub ahead of print]
Combinatorial HLA-peptide bead libraries for high throughput identification of CD8+ T cell specificity.
Pan X, Huang LC, Dong T, Peng Y, Cerundolo V, McGowan S, Ogg G.
Source
MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, NIHR Biomedical Research Centre, University of Oxford, UK.
Abstract
Comprehensive antigenic characterization of a T cell population of unknown specificity is challenging. Existing MHC class I expression systems are limited by the practical difficulty of probing cell populations with an MHC class I peptide library and the cross-reactivity of T cells that are able to recognise many variants of an index peptide. Using emulsion PCR and emulsion in vitro transcription/translation of a random library of peptides conjugated to CD8-null HLA-A*0201 on beads, we probed HLA-A*0201-restricted T cells with specificity for influenza, CMV and EBV. We observed significant enrichment for sequences containing HLA-A2 anchors and correct viral fragments for all T cell populations. HLA bead display provides a novel approach to identify the specificity of T cells.
Copyright ? 2013. Published by Elsevier B.V.
PMID:
24309405
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24309405
Combinatorial HLA-peptide bead libraries for high throughput identification of CD8+ T cell specificity.
Pan X, Huang LC, Dong T, Peng Y, Cerundolo V, McGowan S, Ogg G.
Source
MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, NIHR Biomedical Research Centre, University of Oxford, UK.
Abstract
Comprehensive antigenic characterization of a T cell population of unknown specificity is challenging. Existing MHC class I expression systems are limited by the practical difficulty of probing cell populations with an MHC class I peptide library and the cross-reactivity of T cells that are able to recognise many variants of an index peptide. Using emulsion PCR and emulsion in vitro transcription/translation of a random library of peptides conjugated to CD8-null HLA-A*0201 on beads, we probed HLA-A*0201-restricted T cells with specificity for influenza, CMV and EBV. We observed significant enrichment for sequences containing HLA-A2 anchors and correct viral fragments for all T cell populations. HLA bead display provides a novel approach to identify the specificity of T cells.
Copyright ? 2013. Published by Elsevier B.V.
PMID:
24309405
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24309405