tetano
Editor, Senior Moderator
J Virol. 2012 Aug 22. [Epub ahead of print]
Comparative study of influenza virus replication in MDCK cells and in primary cells derived from adenoids and airway epithelium.
Ilyushina NA, Ikizler MR, Kawaoka Y, Rudenko LG, Treanor JJ, Subbarao K, Wright PF.
Source
Department of Pediatrics, Geisel Medical School at Dartmouth, Lebanon, NH 03756, USA.
Abstract
Although clinical trials in human subjects are essential for determination of safety, infectivity and immunogenicity, it is desirable to know in advance the infectiousness of potential candidates of live attenuated influenza vaccine strains for human use. We compared the replication kinetics of wild-type and live attenuated influenza viruses, including H1N1, H3N2, H9N2 and B strains, in Madin-Darby canine (MDCK) cells, primary epithelial cells derived from human adenoids, and human bronchial epithelium (NHBE). Our data showed that, despite the fact that all tissue culture models lack a functional adaptive immune system differentiated cultures of human epithelium exhibited the greatest restriction for all H1N1, H3N2 and B vaccine viruses studied among three cell types tested and the best correlation with their levels of attenuation seen in clinical trials in humans. In contrast, the data obtained in MDCK cells were the least predictive of restricted viral replication of live attenuated vaccine viruses in humans. We were able to detect a statistically significant difference between replication abilities of the US (A/Ann Arbor/6/60) and Russian (A/Leningrad/134/17/57) cold-adapted vaccine donor strains in NHBE cultures. Since live attenuated pandemic influenza vaccines may potentially express a hemagglutinin and neuraminidase from a non-human influenza virus, we assessed which of the three cell cultures could optimally evaluate infectivity and cellular tropism of viruses derived from different hosts. Among the three cell types tested NHBE cultures most adequately reflected the infectivity and cellular tropism of influenza strains with different receptor specificities. NHBE cultures could be considered as a screening step for evaluating the restricted replication of influenza vaccine candidates.
PMID:
22915797
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22915797
Comparative study of influenza virus replication in MDCK cells and in primary cells derived from adenoids and airway epithelium.
Ilyushina NA, Ikizler MR, Kawaoka Y, Rudenko LG, Treanor JJ, Subbarao K, Wright PF.
Source
Department of Pediatrics, Geisel Medical School at Dartmouth, Lebanon, NH 03756, USA.
Abstract
Although clinical trials in human subjects are essential for determination of safety, infectivity and immunogenicity, it is desirable to know in advance the infectiousness of potential candidates of live attenuated influenza vaccine strains for human use. We compared the replication kinetics of wild-type and live attenuated influenza viruses, including H1N1, H3N2, H9N2 and B strains, in Madin-Darby canine (MDCK) cells, primary epithelial cells derived from human adenoids, and human bronchial epithelium (NHBE). Our data showed that, despite the fact that all tissue culture models lack a functional adaptive immune system differentiated cultures of human epithelium exhibited the greatest restriction for all H1N1, H3N2 and B vaccine viruses studied among three cell types tested and the best correlation with their levels of attenuation seen in clinical trials in humans. In contrast, the data obtained in MDCK cells were the least predictive of restricted viral replication of live attenuated vaccine viruses in humans. We were able to detect a statistically significant difference between replication abilities of the US (A/Ann Arbor/6/60) and Russian (A/Leningrad/134/17/57) cold-adapted vaccine donor strains in NHBE cultures. Since live attenuated pandemic influenza vaccines may potentially express a hemagglutinin and neuraminidase from a non-human influenza virus, we assessed which of the three cell cultures could optimally evaluate infectivity and cellular tropism of viruses derived from different hosts. Among the three cell types tested NHBE cultures most adequately reflected the infectivity and cellular tropism of influenza strains with different receptor specificities. NHBE cultures could be considered as a screening step for evaluating the restricted replication of influenza vaccine candidates.
PMID:
22915797
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22915797