tetano
Editor, Senior Moderator
Virology. 2016 Jun 14;496:138-146. doi: 10.1016/j.virol.2016.06.001. [Epub ahead of print]
[h=1]Comparing the functions of equine and canine influenza H3N8 virus PA-X proteins: Suppression of reporter gene expression and modulation of global host gene expression.[/h] Feng KH[SUP]1[/SUP], Sun M[SUP]2[/SUP], Iketani S[SUP]1[/SUP], Holmes EC[SUP]3[/SUP], Parrish CR[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The influenza PA-X protein is translated from the PA open reading frame from frameshifting and suppresses cellular gene expression due to its ribonuclease activity. We further defined the functional roles of PA-X by comparing PA-X proteins from two related viruses - equine influenza (EIV) and canine influenza (CIV) H3N8 - that differ in a C-terminal truncation and internal mutations. In vitro reporter gene assays revealed that both proteins were able to suppress gene expression. Interestingly, EIV PA-X demonstrated ~50% greater activity compared to CIV PA-X, and we identified the mutations that caused this difference. We used RNA-seq to evaluate the effects of PA-X on host gene expression after transfection into cultured cells. There were no significant differences in this property between EIV and CIV PA-X proteins, but expression of either resulted in the up-regulation of genes when compared to controls, most notably immunity-related proteins, trafficking proteins, and transcription factors.
Copyright ? 2016. Published by Elsevier Inc.
[h=4]KEYWORDS:[/h] Canine H3N8; Equine H3N8; Gene suppression; Influenza; PA-X; RNA-seq
PMID: 27314620 [PubMed - as supplied by publisher]
[h=1]Comparing the functions of equine and canine influenza H3N8 virus PA-X proteins: Suppression of reporter gene expression and modulation of global host gene expression.[/h] Feng KH[SUP]1[/SUP], Sun M[SUP]2[/SUP], Iketani S[SUP]1[/SUP], Holmes EC[SUP]3[/SUP], Parrish CR[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The influenza PA-X protein is translated from the PA open reading frame from frameshifting and suppresses cellular gene expression due to its ribonuclease activity. We further defined the functional roles of PA-X by comparing PA-X proteins from two related viruses - equine influenza (EIV) and canine influenza (CIV) H3N8 - that differ in a C-terminal truncation and internal mutations. In vitro reporter gene assays revealed that both proteins were able to suppress gene expression. Interestingly, EIV PA-X demonstrated ~50% greater activity compared to CIV PA-X, and we identified the mutations that caused this difference. We used RNA-seq to evaluate the effects of PA-X on host gene expression after transfection into cultured cells. There were no significant differences in this property between EIV and CIV PA-X proteins, but expression of either resulted in the up-regulation of genes when compared to controls, most notably immunity-related proteins, trafficking proteins, and transcription factors.
Copyright ? 2016. Published by Elsevier Inc.
[h=4]KEYWORDS:[/h] Canine H3N8; Equine H3N8; Gene suppression; Influenza; PA-X; RNA-seq
PMID: 27314620 [PubMed - as supplied by publisher]