tetano
Editor, Senior Moderator
Anal Chem. 2015 Oct 8. [Epub ahead of print]
[h=1]Comparison of influenza virus particle purification using novel magnetic sulfated cellulose particles with an established centrifugation method for analytics.[/h] Serve A, Pieler MM, Benndorf D, Rapp E, Wolff MW, Reichl U.
[h=3]Abstract[/h] A method for the purification of influenza virus particles using novel magnetic sulfated cellulose particles is presented and compared to an established centrifugation method for analytics. Therefore, purified influenza A virus particles from adherent and suspension MDCK host cell lines were characterized on the protein level with mass spectrometry to compare the viral and residual host cell proteins. Both methods allowed to identify all ten influenza A virus proteins, including low abundant proteins like the matrix protein 2 and non-structural protein 1, with a similar impurity level of host cell proteins. Compared to the centrifugation method, use of the novel magnetic sulfated cellulose particles reduced the influenza A virus particle purification time from 3.5 h to 30 min before mass spectrometry analysis.
PMID: 26447768 [PubMed - as supplied by publisher]
[h=1]Comparison of influenza virus particle purification using novel magnetic sulfated cellulose particles with an established centrifugation method for analytics.[/h] Serve A, Pieler MM, Benndorf D, Rapp E, Wolff MW, Reichl U.
[h=3]Abstract[/h] A method for the purification of influenza virus particles using novel magnetic sulfated cellulose particles is presented and compared to an established centrifugation method for analytics. Therefore, purified influenza A virus particles from adherent and suspension MDCK host cell lines were characterized on the protein level with mass spectrometry to compare the viral and residual host cell proteins. Both methods allowed to identify all ten influenza A virus proteins, including low abundant proteins like the matrix protein 2 and non-structural protein 1, with a similar impurity level of host cell proteins. Compared to the centrifugation method, use of the novel magnetic sulfated cellulose particles reduced the influenza A virus particle purification time from 3.5 h to 30 min before mass spectrometry analysis.
PMID: 26447768 [PubMed - as supplied by publisher]