tetano
Editor, Senior Moderator
J Clin Microbiol. 2014 May 21. pii: JCM.00738-14. [Epub ahead of print]
Comparison of Simplexa? Flu A/B & RSV with Cytospin-Immunofluorescence and Laboratory Developed TaqMan PCR in Predominantly Adult Hospitalized Patients.
Landry ML1, Ferguson D2.
Author information
Abstract
To compare methods, 445 nasopharyngeal samples were tested: 199 were positive (46 RSV, 120 influenza A, and 33 influenza B) and 246 were negative. DFA detected 132 (66.3%), Simplexa direct detected 162 (81.4%), Simplexa using extracts detected 177 (88.9%), and lab-developed TaqMan PCR reference methods detected 199 (100%) positives. Specificities were 99.6%, 100%, 100% and 100%, respectively. Both Simplexa methods were more sensitive than DFA (p=0.0001) but less sensitive than TaqMan RT-PCR (p=0.0001).
Copyright ? 2014, American Society for Microbiology. All Rights Reserved.
PMID:
24850350
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24850350
Comparison of Simplexa? Flu A/B & RSV with Cytospin-Immunofluorescence and Laboratory Developed TaqMan PCR in Predominantly Adult Hospitalized Patients.
Landry ML1, Ferguson D2.
Author information
Abstract
To compare methods, 445 nasopharyngeal samples were tested: 199 were positive (46 RSV, 120 influenza A, and 33 influenza B) and 246 were negative. DFA detected 132 (66.3%), Simplexa direct detected 162 (81.4%), Simplexa using extracts detected 177 (88.9%), and lab-developed TaqMan PCR reference methods detected 199 (100%) positives. Specificities were 99.6%, 100%, 100% and 100%, respectively. Both Simplexa methods were more sensitive than DFA (p=0.0001) but less sensitive than TaqMan RT-PCR (p=0.0001).
Copyright ? 2014, American Society for Microbiology. All Rights Reserved.
PMID:
24850350
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24850350