tetano
Editor, Senior Moderator
Diagn Microbiol Infect Dis. 2018 Dec 4. pii: S0732-8893(18)30687-4. doi: 10.1016/j.diagmicrobio.2018.11.022. [Epub ahead of print]
[h=1]Comparison of the Idylla? Respiratory (IFV-RSV) panel with the GeneXpert Xpert? Flu/RSV assay: a retrospective study with nasopharyngeal and midturbinate samples.[/h] Wouters Y[SUP]1[/SUP], Keyaerts E[SUP]2[/SUP], Rector A[SUP]2[/SUP], Van Even E[SUP]1[/SUP], Vissers S[SUP]3[/SUP], Koletzki D[SUP]4[/SUP], Pattery T[SUP]4[/SUP], Rousseau E[SUP]4[/SUP], Van Ranst M[SUP]2[/SUP], Laffut W[SUP]5[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The objective of this study was to compare the performance of the Idylla? Respiratory (IFV-RSV) panel to the GeneXpert Xpert? Flu/RSV assay and establish the performance of a midturbinate swab compared to nasopharyngeal sampling. Considering GeneXpert? assay as imperfect reference standard, a positive percentage agreement between both assays of 98-100% for influenza A and 96-99% for influenza B could be calculated when 354 nasopharyngeal and 325 midturbinate swabs were retrospectively analyzed. Comparing midturbinate samples to nasopharyngeal specimens of 321 subjects, positive percentage agreement varied from 42% to 94% depending on both target virus and assay used. Negative percentage agreements ranged from 98% to 100% for both methods and sample type comparison. The Idylla? assay showed excellent performance compared to the GeneXpert? assay for the detection of influenza virus. The study also showed a slightly better performance for nasopharyngeal sampling compared to the use of a midturbinate swab.
Copyright ? 2018 Elsevier Inc. All rights reserved.
[h=4]KEYWORDS:[/h] GeneXpert; Idylla; Influenza; PCR; RSV; Respiratory panel
PMID: 30638655 DOI: 10.1016/j.diagmicrobio.2018.11.022
[h=1]Comparison of the Idylla? Respiratory (IFV-RSV) panel with the GeneXpert Xpert? Flu/RSV assay: a retrospective study with nasopharyngeal and midturbinate samples.[/h] Wouters Y[SUP]1[/SUP], Keyaerts E[SUP]2[/SUP], Rector A[SUP]2[/SUP], Van Even E[SUP]1[/SUP], Vissers S[SUP]3[/SUP], Koletzki D[SUP]4[/SUP], Pattery T[SUP]4[/SUP], Rousseau E[SUP]4[/SUP], Van Ranst M[SUP]2[/SUP], Laffut W[SUP]5[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The objective of this study was to compare the performance of the Idylla? Respiratory (IFV-RSV) panel to the GeneXpert Xpert? Flu/RSV assay and establish the performance of a midturbinate swab compared to nasopharyngeal sampling. Considering GeneXpert? assay as imperfect reference standard, a positive percentage agreement between both assays of 98-100% for influenza A and 96-99% for influenza B could be calculated when 354 nasopharyngeal and 325 midturbinate swabs were retrospectively analyzed. Comparing midturbinate samples to nasopharyngeal specimens of 321 subjects, positive percentage agreement varied from 42% to 94% depending on both target virus and assay used. Negative percentage agreements ranged from 98% to 100% for both methods and sample type comparison. The Idylla? assay showed excellent performance compared to the GeneXpert? assay for the detection of influenza virus. The study also showed a slightly better performance for nasopharyngeal sampling compared to the use of a midturbinate swab.
Copyright ? 2018 Elsevier Inc. All rights reserved.
[h=4]KEYWORDS:[/h] GeneXpert; Idylla; Influenza; PCR; RSV; Respiratory panel
PMID: 30638655 DOI: 10.1016/j.diagmicrobio.2018.11.022