tetano
Editor, Senior Moderator
J Med Microbiol. 2015 Jan 13. pii: jmm.0.000007. doi: 10.1099/jmm.0.000007. [Epub ahead of print]
[h=1]Comparison of Turnaround Time (TAT) and Time to Oseltamivir Discontinuation between Two Respiratory Viral Panel Testing (RVP) Methodologies.[/h] Pettit N[SUP]1[/SUP], Matushek S[SUP]1[/SUP], Charnot-Katsikas A[SUP]1[/SUP], Tesic V[SUP]1[/SUP], Boonlayangoor S[SUP]1[/SUP], Brielmaier B[SUP]1[/SUP], Pisano J[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Multiplex PCR is highly sensitive and provides a more rapid turnaround time (TAT) compared to other respiratory viral testing methodologies. Our microbiology laboratory switched from the Luminex xTAG? RVP (detects 12 respiratory viruses with an assay time of 8.5 hours) to the Biofire Diagnostics, Inc. FilmArray?-RP (detects 17 respiratory viral and 3 bacterial targets with an assay time of 1.2 hours). We sought to compare the TAT between the two respiratory viral panels (RVP) performed at different frequencies and determine time to discontinuation of empiric oseltamivir. All adult patients with an RVP test result reported between 12/1/2011-2/28/2012 performed on Luminex xTAG? RVP (2-3 times/week) and 12/1/2012-2/28/2013 performed on FilmArray?-RP (24 hours/day, 7 days/week) were evaluated for average TAT. Among patients with influenza negative RVP results, time to discontinuation of empiric oseltamivir was determined. Average TAT for the Luminex xTAG? RVP performed 2-3x/week between 12/1/2011-2/28/2012 (N=230 assays) was 46.4 hours compared to an average TAT of 3.1 hours (p<0.001) for FilmArray?-RP performed 24 hours a day/7 days per week from 12/1/2012-2/28/13 (N=872 assays). The average time to discontinuation of empiric oseltamivir among patients with an RVP negative for influenza was 4 and 2 days for the Luminex? xTAG (N=42) and FilmArray? (N=75) groups, respectively (p<0.001). Consistent with previous literature showing that the TAT for FilmArray?-RP is, we observed a significantly shorter average TAT. We also found that the average duration of empiric oseltamivir was reduced by 50% with FilmArray?-RP.
PMID: 25587073 [PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/25587073
[h=1]Comparison of Turnaround Time (TAT) and Time to Oseltamivir Discontinuation between Two Respiratory Viral Panel Testing (RVP) Methodologies.[/h] Pettit N[SUP]1[/SUP], Matushek S[SUP]1[/SUP], Charnot-Katsikas A[SUP]1[/SUP], Tesic V[SUP]1[/SUP], Boonlayangoor S[SUP]1[/SUP], Brielmaier B[SUP]1[/SUP], Pisano J[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Multiplex PCR is highly sensitive and provides a more rapid turnaround time (TAT) compared to other respiratory viral testing methodologies. Our microbiology laboratory switched from the Luminex xTAG? RVP (detects 12 respiratory viruses with an assay time of 8.5 hours) to the Biofire Diagnostics, Inc. FilmArray?-RP (detects 17 respiratory viral and 3 bacterial targets with an assay time of 1.2 hours). We sought to compare the TAT between the two respiratory viral panels (RVP) performed at different frequencies and determine time to discontinuation of empiric oseltamivir. All adult patients with an RVP test result reported between 12/1/2011-2/28/2012 performed on Luminex xTAG? RVP (2-3 times/week) and 12/1/2012-2/28/2013 performed on FilmArray?-RP (24 hours/day, 7 days/week) were evaluated for average TAT. Among patients with influenza negative RVP results, time to discontinuation of empiric oseltamivir was determined. Average TAT for the Luminex xTAG? RVP performed 2-3x/week between 12/1/2011-2/28/2012 (N=230 assays) was 46.4 hours compared to an average TAT of 3.1 hours (p<0.001) for FilmArray?-RP performed 24 hours a day/7 days per week from 12/1/2012-2/28/13 (N=872 assays). The average time to discontinuation of empiric oseltamivir among patients with an RVP negative for influenza was 4 and 2 days for the Luminex? xTAG (N=42) and FilmArray? (N=75) groups, respectively (p<0.001). Consistent with previous literature showing that the TAT for FilmArray?-RP is, we observed a significantly shorter average TAT. We also found that the average duration of empiric oseltamivir was reduced by 50% with FilmArray?-RP.
PMID: 25587073 [PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/25587073