tetano
Editor, Senior Moderator
BMC Infect Dis. 2019 Aug 20;19(1):729. doi: 10.1186/s12879-019-4385-5.
[h=1]Comparison of viral and epidemiological profiles of hospitalized children with severe acute respiratory infection in Beijing and Shanghai, China.[/h] Zhao Y[SUP]1,[/SUP][SUP]2[/SUP], Lu R[SUP]2[/SUP], Shen J[SUP]3[/SUP], Xie Z[SUP]4[/SUP], Liu G[SUP]1[/SUP], Tan W[SUP]5,[/SUP][SUP]6[/SUP].
[h=3]Author information[/h] 1 Key Laboratory of Laboratory Medicine, Ministry of Education, and Institute of Medical Virology, Wenzhou Medical University, Zhejiang, China. 2 National Institute for Viral Disease Control and Prevention, China CDC, 155Changbai Road, Beijing, 102206, Changping District, China. 3 Children's Hospital of Fudan University, Shanghai, China. 4 Key Laboratory of Major Diseases in Children and National Key Discipline of Pediatrics (Capital Medical University), Ministry of Education, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, Beijing, 100045, China. 5 Key Laboratory of Laboratory Medicine, Ministry of Education, and Institute of Medical Virology, Wenzhou Medical University, Zhejiang, China. tanwj28@163.com. 6 National Institute for Viral Disease Control and Prevention, China CDC, 155Changbai Road, Beijing, 102206, Changping District, China. tanwj28@163.com.
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] No comparison data have been reported on viral and epidemiological profiles of hospitalized children with severe acute respiratory infection (SARI) in Beijing or Shanghai, China.
[h=4]METHODS:[/h] We collected 700 nasopharyngeal aspirates (NPA) from hospitalized children with SARI in Beijing (northern China) and Shanghai (southern China). Multiple respiratory viruses (including 15 common viruses) were screened by validated polymerase chain reaction (PCR) or real-time reverse transcription-PCR assays and confirmed by sequencing. Demographic data and the distribution of viral infections were also examined.
[h=4]RESULTS:[/h] Of 700 samples, 547 (78.1%) tested positive for viral infections. The picornaviruses (PIC), which included rhinovirus (RV) and enterovirus (EV), were the most common (34.0%), followed by respiratory syncytial virus (RSV) (28.3%), human bocavirus (HBoV) (19.1%), adenovirus (ADV) (13.7%), human coronaviruses (HCoV) (10.7%), influenza A and B (8.9%), parainfluenza virus (PIV 1-3) (7.9%), and human metapneumovirus (HMPV) (5.0%). PIC (RV/EV) and RSV were the most prevalent etiological agents of SARI in both cities. The total and age-matched prevalence of RSV, HCoV, and hMPV among SARI children under 5 years old were significantly higher in Beijing than in Shanghai. Different age and seasonal distribution patterns of the viral infections were found between Beijing and Shanghai.
[h=4]CONCLUSIONS:[/h] Viral infection was tested and shown to be the most prevalent etiological agent among children with SARI in either the Beijing or the Shanghai area, while showing different patterns of viral and epidemiological profiles. Our findings provide a better understanding of the roles of geographic location and climate in respiratory viral infections in hospitalized children with SARI.
[h=4]KEYWORDS:[/h] Beijing; Children; Epidemiological profile; Nasopharyngeal aspirates; Polymerase chain reaction; Severe acute respiratory infection; Shanghai; Virus
PMID: 31429710 DOI: 10.1186/s12879-019-4385-5
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[h=1]Comparison of viral and epidemiological profiles of hospitalized children with severe acute respiratory infection in Beijing and Shanghai, China.[/h] Zhao Y[SUP]1,[/SUP][SUP]2[/SUP], Lu R[SUP]2[/SUP], Shen J[SUP]3[/SUP], Xie Z[SUP]4[/SUP], Liu G[SUP]1[/SUP], Tan W[SUP]5,[/SUP][SUP]6[/SUP].
[h=3]Author information[/h] 1 Key Laboratory of Laboratory Medicine, Ministry of Education, and Institute of Medical Virology, Wenzhou Medical University, Zhejiang, China. 2 National Institute for Viral Disease Control and Prevention, China CDC, 155Changbai Road, Beijing, 102206, Changping District, China. 3 Children's Hospital of Fudan University, Shanghai, China. 4 Key Laboratory of Major Diseases in Children and National Key Discipline of Pediatrics (Capital Medical University), Ministry of Education, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, Beijing, 100045, China. 5 Key Laboratory of Laboratory Medicine, Ministry of Education, and Institute of Medical Virology, Wenzhou Medical University, Zhejiang, China. tanwj28@163.com. 6 National Institute for Viral Disease Control and Prevention, China CDC, 155Changbai Road, Beijing, 102206, Changping District, China. tanwj28@163.com.
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] No comparison data have been reported on viral and epidemiological profiles of hospitalized children with severe acute respiratory infection (SARI) in Beijing or Shanghai, China.
[h=4]METHODS:[/h] We collected 700 nasopharyngeal aspirates (NPA) from hospitalized children with SARI in Beijing (northern China) and Shanghai (southern China). Multiple respiratory viruses (including 15 common viruses) were screened by validated polymerase chain reaction (PCR) or real-time reverse transcription-PCR assays and confirmed by sequencing. Demographic data and the distribution of viral infections were also examined.
[h=4]RESULTS:[/h] Of 700 samples, 547 (78.1%) tested positive for viral infections. The picornaviruses (PIC), which included rhinovirus (RV) and enterovirus (EV), were the most common (34.0%), followed by respiratory syncytial virus (RSV) (28.3%), human bocavirus (HBoV) (19.1%), adenovirus (ADV) (13.7%), human coronaviruses (HCoV) (10.7%), influenza A and B (8.9%), parainfluenza virus (PIV 1-3) (7.9%), and human metapneumovirus (HMPV) (5.0%). PIC (RV/EV) and RSV were the most prevalent etiological agents of SARI in both cities. The total and age-matched prevalence of RSV, HCoV, and hMPV among SARI children under 5 years old were significantly higher in Beijing than in Shanghai. Different age and seasonal distribution patterns of the viral infections were found between Beijing and Shanghai.
[h=4]CONCLUSIONS:[/h] Viral infection was tested and shown to be the most prevalent etiological agent among children with SARI in either the Beijing or the Shanghai area, while showing different patterns of viral and epidemiological profiles. Our findings provide a better understanding of the roles of geographic location and climate in respiratory viral infections in hospitalized children with SARI.
[h=4]KEYWORDS:[/h] Beijing; Children; Epidemiological profile; Nasopharyngeal aspirates; Polymerase chain reaction; Severe acute respiratory infection; Shanghai; Virus
PMID: 31429710 DOI: 10.1186/s12879-019-4385-5
Free full text