tetano
Editor, Senior Moderator
J Virol. 2011 Oct 12. [Epub ahead of print]
Complement-Dependent Lysis of Influenza A Virus-Infected Cells by Broadly Cross-Reactive Human Monoclonal Antibodies.
Terajima M, Cruz J, Co MD, Lee JH, Kaur K, Wilson PC, Ennis FA.
Source
Center for Infectious Disease and Vaccine Research, Division of Infectious Diseases and Immunology, Department of Medicine, University of Massachusetts Medical School, Worcester, Massachusetts.
Abstract
We characterized human monoclonal antibodies (mAbs) cloned from influenza virus-infected patients and from influenza vaccine recipients in complement-dependent lysis (CDL) assays. Most mAbs active in CDL were neutralizing, but not all neutralizing mAbs can mediate CDL. Two of the three stalk-specific neutralizing mAbs tested were able to mediate CDL and were more cross-reactive to temporally distant H1N1 strains than the conventional hemaggulutination-inhibiting and neutralizing mAbs. One of the stalk-specific mAbs was subtype-cross-reactive to H1 and H2 hemagglutinins, suggesting a role for stalk-specific antibodies in protection against influenza illness, especially by a novel subtype which can cause pandemics.
PMID:
21994454
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/21994454
Complement-Dependent Lysis of Influenza A Virus-Infected Cells by Broadly Cross-Reactive Human Monoclonal Antibodies.
Terajima M, Cruz J, Co MD, Lee JH, Kaur K, Wilson PC, Ennis FA.
Source
Center for Infectious Disease and Vaccine Research, Division of Infectious Diseases and Immunology, Department of Medicine, University of Massachusetts Medical School, Worcester, Massachusetts.
Abstract
We characterized human monoclonal antibodies (mAbs) cloned from influenza virus-infected patients and from influenza vaccine recipients in complement-dependent lysis (CDL) assays. Most mAbs active in CDL were neutralizing, but not all neutralizing mAbs can mediate CDL. Two of the three stalk-specific neutralizing mAbs tested were able to mediate CDL and were more cross-reactive to temporally distant H1N1 strains than the conventional hemaggulutination-inhibiting and neutralizing mAbs. One of the stalk-specific mAbs was subtype-cross-reactive to H1 and H2 hemagglutinins, suggesting a role for stalk-specific antibodies in protection against influenza illness, especially by a novel subtype which can cause pandemics.
PMID:
21994454
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/21994454