tetano
Editor, Senior Moderator
Influenza Other Respir Viruses. 2018 Dec 11. doi: 10.1111/irv.12624. [Epub ahead of print]
[h=1]Consecutive influenza surveillance of neuraminidase mutations and neuraminidase inhibitor resistance in Japan.[/h] Chong Y[SUP]1[/SUP], Matsumoto S[SUP]2[/SUP], Kang D[SUP]2,[/SUP][SUP]3[/SUP], Ikematsu H[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] The large consumption of neuraminidase (NA) inhibitors (NAIs) for the treatment of influenza virus infections places Japan at risk of becoming the epicenter of the global spread of NAI-resistant viruses.
[h=4]OBJECTIVE:[/h] To clarify NA amino acid mutations of epidemic influenza viruses in Japan and their related NAI resistance METHODS: A total of 1791 samples, including 396 A/H1N1pdm09, 1117 A/H3N2, and 278 B isolates, were collected to determine of their 50% inhibitory concentration (IC[SUB]50[/SUB] ) values by NAIs (oseltamivir, zanamivir, peramivir, and laninamivir) during the Japanese seasons from 2011-12 to 2016-17. Then, 380 samples including 49 A/H1N1pdm09, 251 A/H3N2, and 80 B isolates, were sequenced for the entire NA genes.
[h=4]RESULTS:[/h] NAI-resistant A/H1N1pdm09 viruses were detected at a frequency of 1.3% (5/396 isolates) in the epidemic seasons. None of the A/H3N2 and B viruses developed resistance to any of the four NAIs during the six seasons. Only five and 13 AA mutations were detected in the NA catalytic sites of A/H1N1pdm09 and A/H3N2 viruses, respectively. No mutations were observed in the catalytic sites of B viruses. Four of the five mutations in the catalytic sites of A/H1N1pdm09 consisted of H275Y, which was related to high resistance to oseltamivir and peramivir. Most (10/13) of the catalytic site mutations in A/H3N2 were associated with MDCK-passaged induction (D151G/N). Finally, no mutations related to substantial NAI resistance were detected in the A/H3N2 and B viruses examined.
[h=4]CONCLUSION:[/h] These findings suggest that the NA catalytic sites of influenza viruses are well preserved. Even in Japan, no spread of NAI-resistant viruses has been observed, and A/H1N1pdm09 viruses carrying H275Y remain limited. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
[h=4]KEYWORDS:[/h] Influenza; mutation; neuraminidase; neuraminidase inhibitor; resistance
PMID: 30548432 DOI: 10.1111/irv.12624
[h=1]Consecutive influenza surveillance of neuraminidase mutations and neuraminidase inhibitor resistance in Japan.[/h] Chong Y[SUP]1[/SUP], Matsumoto S[SUP]2[/SUP], Kang D[SUP]2,[/SUP][SUP]3[/SUP], Ikematsu H[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] The large consumption of neuraminidase (NA) inhibitors (NAIs) for the treatment of influenza virus infections places Japan at risk of becoming the epicenter of the global spread of NAI-resistant viruses.
[h=4]OBJECTIVE:[/h] To clarify NA amino acid mutations of epidemic influenza viruses in Japan and their related NAI resistance METHODS: A total of 1791 samples, including 396 A/H1N1pdm09, 1117 A/H3N2, and 278 B isolates, were collected to determine of their 50% inhibitory concentration (IC[SUB]50[/SUB] ) values by NAIs (oseltamivir, zanamivir, peramivir, and laninamivir) during the Japanese seasons from 2011-12 to 2016-17. Then, 380 samples including 49 A/H1N1pdm09, 251 A/H3N2, and 80 B isolates, were sequenced for the entire NA genes.
[h=4]RESULTS:[/h] NAI-resistant A/H1N1pdm09 viruses were detected at a frequency of 1.3% (5/396 isolates) in the epidemic seasons. None of the A/H3N2 and B viruses developed resistance to any of the four NAIs during the six seasons. Only five and 13 AA mutations were detected in the NA catalytic sites of A/H1N1pdm09 and A/H3N2 viruses, respectively. No mutations were observed in the catalytic sites of B viruses. Four of the five mutations in the catalytic sites of A/H1N1pdm09 consisted of H275Y, which was related to high resistance to oseltamivir and peramivir. Most (10/13) of the catalytic site mutations in A/H3N2 were associated with MDCK-passaged induction (D151G/N). Finally, no mutations related to substantial NAI resistance were detected in the A/H3N2 and B viruses examined.
[h=4]CONCLUSION:[/h] These findings suggest that the NA catalytic sites of influenza viruses are well preserved. Even in Japan, no spread of NAI-resistant viruses has been observed, and A/H1N1pdm09 viruses carrying H275Y remain limited. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
[h=4]KEYWORDS:[/h] Influenza; mutation; neuraminidase; neuraminidase inhibitor; resistance
PMID: 30548432 DOI: 10.1111/irv.12624