tetano
Editor, Senior Moderator
J Med Virol. 2016 Apr 21. doi: 10.1002/jmv.24558. [Epub ahead of print]
[h=1]Construction high-yield candidate influenza vaccine viruses in Vero cells by reassortment.[/h] Yu W[SUP]1[/SUP], Yang F[SUP]1[/SUP], Yang J[SUP]1[/SUP], Ma L[SUP]1[/SUP], Cun Y[SUP]1[/SUP], Song S[SUP]1[/SUP], Liao G[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Usage of influenza vaccine is the best choice measure for preventing and conclusion of influenza virus infection. Although it has been used of chicken embryo to produce influenza vaccine, following with WHO recommended vaccine strain, there were uncontrollable factors and its deficiencies, specially, during an influenza pandemic in the world. The Vero cells are used for vaccine production of a few strains including influenza virus, because of its homology with human, recommended by WHO. However, as known most of the influenza viruses strains couldn't culture by Vero cells. It was used two high-yield influenza viruses adapted in Vero cells as donor viruses, such as A/Yunnan/1/2005Va (H3N2) and B/Yunnan/2/2005Va (B), to construct high-yield wild influenza virus in Vero cells under antibody selection pressure. After reassortment and passages, it obtained the new Vaccine strains with A/Tianjin/15/2009 Va (H1N1), A/Fujian /196/2009 Va (H3N2) and B/Chongqing/1384/2010 Va (B), which was not only completely keeping their original antigenic (HA and NA), but also grown well in Vero cells with high-yield. All results of gene analysis and HA, HI shown that this reassortment method could be used to find new direction to product the influenza vaccine. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
[h=4]KEYWORDS:[/h] Influenza vaccine; Vero cells adaption; reassortment
PMID: 27101353 [PubMed - as supplied by publisher]
[h=1]Construction high-yield candidate influenza vaccine viruses in Vero cells by reassortment.[/h] Yu W[SUP]1[/SUP], Yang F[SUP]1[/SUP], Yang J[SUP]1[/SUP], Ma L[SUP]1[/SUP], Cun Y[SUP]1[/SUP], Song S[SUP]1[/SUP], Liao G[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Usage of influenza vaccine is the best choice measure for preventing and conclusion of influenza virus infection. Although it has been used of chicken embryo to produce influenza vaccine, following with WHO recommended vaccine strain, there were uncontrollable factors and its deficiencies, specially, during an influenza pandemic in the world. The Vero cells are used for vaccine production of a few strains including influenza virus, because of its homology with human, recommended by WHO. However, as known most of the influenza viruses strains couldn't culture by Vero cells. It was used two high-yield influenza viruses adapted in Vero cells as donor viruses, such as A/Yunnan/1/2005Va (H3N2) and B/Yunnan/2/2005Va (B), to construct high-yield wild influenza virus in Vero cells under antibody selection pressure. After reassortment and passages, it obtained the new Vaccine strains with A/Tianjin/15/2009 Va (H1N1), A/Fujian /196/2009 Va (H3N2) and B/Chongqing/1384/2010 Va (B), which was not only completely keeping their original antigenic (HA and NA), but also grown well in Vero cells with high-yield. All results of gene analysis and HA, HI shown that this reassortment method could be used to find new direction to product the influenza vaccine. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
[h=4]KEYWORDS:[/h] Influenza vaccine; Vero cells adaption; reassortment
PMID: 27101353 [PubMed - as supplied by publisher]