tetano
Editor, Senior Moderator
Cytokine
. 2022 Apr 25;154:155894.
doi: 10.1016/j.cyto.2022.155894. Online ahead of print.
Cytokine response over the course of COVID-19 infection in pregnant women
Daniel B Rosen[SUP] 1 [/SUP], Elisabeth A Murphy[SUP] 2 [/SUP], Ron S Gejman[SUP] 1 [/SUP], Allyson Capili[SUP] 1 [/SUP], Rachel L Friedlander[SUP] 1 [/SUP], Sophie Rand[SUP] 2 [/SUP], Kristen A Cagino[SUP] 3 [/SUP], Shannon M Glynn[SUP] 1 [/SUP], Kathy C Matthews[SUP] 3 [/SUP], Jeff M Kubiak[SUP] 2 [/SUP], Jim Yee[SUP] 4 [/SUP], Malavika Prabhu[SUP] 3 [/SUP], Laura E Riley[SUP] 3 [/SUP], Yawei J Yang[SUP] 5 [/SUP]
Affiliations
Abstract
Objective: To study how severity and progression of coronavirus disease (COVID-19) affect cytokine profiles in pregnant women.
Materials and methods: 69 third-trimester, pregnant women were tested for COVID-19 infection and SARS-CoV-2 specific IgM and IgG antibodies. Patients were stratified according to SARS-CoV-2 Reverse Transcriptase-PCR (RT-PCR) status and serology (IgM and IgG) status. Cytokines G-CSF, HGF, IL-18, IL-1Ra, IL-2Ra, IL-8, and IP-10 were measured via ELISA. Retrospective chart review for COVID-19 symptoms and patient vitals was conducted, and cytokine levels were compared between SARS-CoV-2 positive and negative cohorts, by seronegative and seropositive infection, by time course since onset of infection, and according to NIH defined clinical severity.
Results: IL-18, IL-1Ra, and IP-10 increased in the 44 RT-PCR positive pregnant women compared to the 25 RT-PCR negative pregnant controls. Elevated cytokine levels were found in early infections, defined by positive RT-PCR and seronegative status, and higher cytokine levels were also associated with more severe disease. By IgM seroconversion, IL-8 and IP-10 returned to levels seen in uninfected patients, while IL-18 levels remained significantly elevated.
Conclusion: Cytokine profiles of third-trimester pregnant women vary with the time course of infection and are correlated with clinical severity.
Keywords: Cytokine profile; Interferon; Interleukin; Pregnancy; SARS-CoV-2.
. 2022 Apr 25;154:155894.
doi: 10.1016/j.cyto.2022.155894. Online ahead of print.
Cytokine response over the course of COVID-19 infection in pregnant women
Daniel B Rosen[SUP] 1 [/SUP], Elisabeth A Murphy[SUP] 2 [/SUP], Ron S Gejman[SUP] 1 [/SUP], Allyson Capili[SUP] 1 [/SUP], Rachel L Friedlander[SUP] 1 [/SUP], Sophie Rand[SUP] 2 [/SUP], Kristen A Cagino[SUP] 3 [/SUP], Shannon M Glynn[SUP] 1 [/SUP], Kathy C Matthews[SUP] 3 [/SUP], Jeff M Kubiak[SUP] 2 [/SUP], Jim Yee[SUP] 4 [/SUP], Malavika Prabhu[SUP] 3 [/SUP], Laura E Riley[SUP] 3 [/SUP], Yawei J Yang[SUP] 5 [/SUP]
Affiliations
- PMID: 35490452
- DOI: 10.1016/j.cyto.2022.155894
Abstract
Objective: To study how severity and progression of coronavirus disease (COVID-19) affect cytokine profiles in pregnant women.
Materials and methods: 69 third-trimester, pregnant women were tested for COVID-19 infection and SARS-CoV-2 specific IgM and IgG antibodies. Patients were stratified according to SARS-CoV-2 Reverse Transcriptase-PCR (RT-PCR) status and serology (IgM and IgG) status. Cytokines G-CSF, HGF, IL-18, IL-1Ra, IL-2Ra, IL-8, and IP-10 were measured via ELISA. Retrospective chart review for COVID-19 symptoms and patient vitals was conducted, and cytokine levels were compared between SARS-CoV-2 positive and negative cohorts, by seronegative and seropositive infection, by time course since onset of infection, and according to NIH defined clinical severity.
Results: IL-18, IL-1Ra, and IP-10 increased in the 44 RT-PCR positive pregnant women compared to the 25 RT-PCR negative pregnant controls. Elevated cytokine levels were found in early infections, defined by positive RT-PCR and seronegative status, and higher cytokine levels were also associated with more severe disease. By IgM seroconversion, IL-8 and IP-10 returned to levels seen in uninfected patients, while IL-18 levels remained significantly elevated.
Conclusion: Cytokine profiles of third-trimester pregnant women vary with the time course of infection and are correlated with clinical severity.
Keywords: Cytokine profile; Interferon; Interleukin; Pregnancy; SARS-CoV-2.