tetano
Editor, Senior Moderator
J Clin Microbiol. 2017 Jan 11. pii: JCM.02049-16. doi: 10.1128/JCM.02049-16. [Epub ahead of print]
[h=1]Detection of Antigenic Variants of Swine Subtype H3 Influenza A Viruses from Clinical Samples.[/h] Martin BE[SUP]1[/SUP], Bowman AS[SUP]2[/SUP], Li L[SUP]1[/SUP], Nolting JM[SUP]2[/SUP], Smith DR[SUP]3[/SUP], Hanson LA[SUP]1[/SUP], Wan XF[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] A large population of genetically and antigenically diverse influenza A viruses (IAVs) are circulating among the swine population, playing an important role in influenza ecology. Swine IAVs not only cause outbreaks among swine, but they can also be transmitted to humans, causing sporadic infections and even pandemic outbreaks. Antigenic characterization of swine IAVs are key to understanding the natural history of these viruses in swine and to selecting strains for effective vaccines. However, influenza outbreaks generally spread rapidly among swine, and the conventional methods for antigenic characterization require virus propagation, a time-consuming process that can significantly reduce the effectiveness of vaccination programs. We developed and validated a rapid, sensitive, and robust method, the polyclonal sera-based proximity ligation assay (polyPLA), to identify antigenic variants of subtype H3N2 swine IAVs. This method utilizes oligonucleotide-conjugated polyclonal antibodies and quantifies antibody-antigen binding affinities by quantitative RT-PCR. Results showed the assay can rapidly detect H3N2 IAVs directly from nasal wash or nasal swab samples collected from laboratory-challenged animals or during influenza surveillance at county fairs. In addition, polyPLA can accurately separate the viruses at two contemporary swine IAV antigenic clusters (H3N2 swine IAV-α and H3N2 swine IAV-?) with a sensitivity of 84.9% and a specificity of 100.0%. The polyPLA can be routinely used in surveillance programs to detect antigenic variants of influenza viruses and to select vaccine strains for use in controlling and preventing disease in swine.
Copyright ? 2017 American Society for Microbiology.
PMID: 28077698 DOI: 10.1128/JCM.02049-16
[PubMed - as supplied by publisher]
[h=1]Detection of Antigenic Variants of Swine Subtype H3 Influenza A Viruses from Clinical Samples.[/h] Martin BE[SUP]1[/SUP], Bowman AS[SUP]2[/SUP], Li L[SUP]1[/SUP], Nolting JM[SUP]2[/SUP], Smith DR[SUP]3[/SUP], Hanson LA[SUP]1[/SUP], Wan XF[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] A large population of genetically and antigenically diverse influenza A viruses (IAVs) are circulating among the swine population, playing an important role in influenza ecology. Swine IAVs not only cause outbreaks among swine, but they can also be transmitted to humans, causing sporadic infections and even pandemic outbreaks. Antigenic characterization of swine IAVs are key to understanding the natural history of these viruses in swine and to selecting strains for effective vaccines. However, influenza outbreaks generally spread rapidly among swine, and the conventional methods for antigenic characterization require virus propagation, a time-consuming process that can significantly reduce the effectiveness of vaccination programs. We developed and validated a rapid, sensitive, and robust method, the polyclonal sera-based proximity ligation assay (polyPLA), to identify antigenic variants of subtype H3N2 swine IAVs. This method utilizes oligonucleotide-conjugated polyclonal antibodies and quantifies antibody-antigen binding affinities by quantitative RT-PCR. Results showed the assay can rapidly detect H3N2 IAVs directly from nasal wash or nasal swab samples collected from laboratory-challenged animals or during influenza surveillance at county fairs. In addition, polyPLA can accurately separate the viruses at two contemporary swine IAV antigenic clusters (H3N2 swine IAV-α and H3N2 swine IAV-?) with a sensitivity of 84.9% and a specificity of 100.0%. The polyPLA can be routinely used in surveillance programs to detect antigenic variants of influenza viruses and to select vaccine strains for use in controlling and preventing disease in swine.
Copyright ? 2017 American Society for Microbiology.
PMID: 28077698 DOI: 10.1128/JCM.02049-16
[PubMed - as supplied by publisher]