Mary Wilson
Well-known member
February 2008
DOI:10.1089/vbz.2007.0142
PHILIPPE BARBAZAN,1,2, ARUNEE THITITHANYANONT,1,3, DOROTHÉE MISSÉ,2,4, AUDREY DUBOT,1,2, PRISCILLE BOSC,5, NATSUANG LUANGSRI,1, JEAN-PAUL GONZALEZ, 1,2 and PATTAMAPORN KITTAYAPONG1,6
ABSTRACT
Blood-engorged mosquitoes were collected at poultry farms during an outbreak of highly pathogenic avian in- fluenza in Central Thailand during October 2005. These mosquitoes tested positive for H5N1 virus by reverse transcription-polymerase chain reaction (RT-PCR). Results were confirmed by limited sequencing of the H5 and N1 segments. Infection and replication of this virus in the C6/36 mosquito cell line was confirmed by quantitative real-time PCR. However, transmission by mosquitoes was not evaluated, and further research is needed. Collecting and testing mosquitoes engorged with the blood of domestic or wild animals could be a valuable tool for veterinary and public health authorities who conduct surveillance for H5N1 virus spread.
https://www.researchgate.net/public...ected_in_an_Infected_Poultry_Farm_in_Thailand
DOI:10.1089/vbz.2007.0142
PHILIPPE BARBAZAN,1,2, ARUNEE THITITHANYANONT,1,3, DOROTHÉE MISSÉ,2,4, AUDREY DUBOT,1,2, PRISCILLE BOSC,5, NATSUANG LUANGSRI,1, JEAN-PAUL GONZALEZ, 1,2 and PATTAMAPORN KITTAYAPONG1,6
ABSTRACT
Blood-engorged mosquitoes were collected at poultry farms during an outbreak of highly pathogenic avian in- fluenza in Central Thailand during October 2005. These mosquitoes tested positive for H5N1 virus by reverse transcription-polymerase chain reaction (RT-PCR). Results were confirmed by limited sequencing of the H5 and N1 segments. Infection and replication of this virus in the C6/36 mosquito cell line was confirmed by quantitative real-time PCR. However, transmission by mosquitoes was not evaluated, and further research is needed. Collecting and testing mosquitoes engorged with the blood of domestic or wild animals could be a valuable tool for veterinary and public health authorities who conduct surveillance for H5N1 virus spread.
https://www.researchgate.net/public...ected_in_an_Infected_Poultry_Farm_in_Thailand