tetano
Editor, Senior Moderator
Influenza Other Respir Viruses. 2015 Feb 27. doi: 10.1111/irv.12303. [Epub ahead of print]
[h=1]Detection of influenza A and B with the Alere? i Influenza A & B: a novel isothermal nucleic acid amplification assay.[/h] Hazelton B[SUP]1[/SUP], Gray T, Ho J, Ratnamohan VM, Dwyer DE, Kok J.
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] Rapid influenza diagnostic tests (RIDTs) have an important role in clinical decision-making; however, the performances of currently available assays vary widely.
[h=4]OBJECTIVES:[/h] We evaluated the performance of the Alere[SUP]?[/SUP] i Influenza A&B (Alere[SUP]?[/SUP] iNAT), a rapid isothermal nucleic acid amplification assay that has recently received FDA clearance, for the detection of influenza A and B viruses during the Australian influenza season of 2013. Results were compared to two other RIDTs tested in parallel; Quidel Sofia[SUP]?[/SUP] Influenza A+B fluorescent immunoassay (FIA) and Alere[SUP]?[/SUP] BinaxNOW[SUP]?[/SUP] Influenza A & B immunochromatographic (ICT) assay.
[h=4]METHODS:[/h] A total of 202 paired nasopharyngeal swabs collected from patients ≥16 years old with an influenza-like illness (ILI) were eluted in 2 ml of universal transport medium (UTM) that was used to perform all three RIDTs in parallel. Reverse-transcription polymerase chain reaction (RT-PCR) was used as the reference standard.
[h=4]RESULTS:[/h] Compared to RT-PCR, Alere[SUP]?[/SUP] iNAT detected 77?8% influenza A positive samples versus 71?4% and 44?4% for the Quidel Sofia[SUP]?[/SUP] Influenza A+B FIA and BinaxNOW[SUP]?[/SUP] Influenza A & B ICT assay, respectively. For influenza B, Alere[SUP]?[/SUP] iNAT detected 75% of those positive by RT-PCR, versus 33?3% and 25?0% for Sofia[SUP]?[/SUP] and BinaxNOW[SUP]?[/SUP] , respectively. The specificity of Alere[SUP]?[/SUP] iNAT was 100% for influenza A and 99% for influenza B.
[h=4]CONCLUSIONS:[/h] Alere[SUP]?[/SUP] i Influenza A&B is a promising new rapid influenza diagnostic assay with potential point-of-care applications.
? 2015 The Authors. Influenza and Other Respiratory Viruses Published by John Wiley & Sons Ltd.
[h=4]KEYWORDS:[/h] Influenza; isothermal nucleic acid amplification; rapid diagnostic test(s); sensitivity; specificity
PMID: 25728758 [PubMed - as supplied by publisher]
[h=1]Detection of influenza A and B with the Alere? i Influenza A & B: a novel isothermal nucleic acid amplification assay.[/h] Hazelton B[SUP]1[/SUP], Gray T, Ho J, Ratnamohan VM, Dwyer DE, Kok J.
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] Rapid influenza diagnostic tests (RIDTs) have an important role in clinical decision-making; however, the performances of currently available assays vary widely.
[h=4]OBJECTIVES:[/h] We evaluated the performance of the Alere[SUP]?[/SUP] i Influenza A&B (Alere[SUP]?[/SUP] iNAT), a rapid isothermal nucleic acid amplification assay that has recently received FDA clearance, for the detection of influenza A and B viruses during the Australian influenza season of 2013. Results were compared to two other RIDTs tested in parallel; Quidel Sofia[SUP]?[/SUP] Influenza A+B fluorescent immunoassay (FIA) and Alere[SUP]?[/SUP] BinaxNOW[SUP]?[/SUP] Influenza A & B immunochromatographic (ICT) assay.
[h=4]METHODS:[/h] A total of 202 paired nasopharyngeal swabs collected from patients ≥16 years old with an influenza-like illness (ILI) were eluted in 2 ml of universal transport medium (UTM) that was used to perform all three RIDTs in parallel. Reverse-transcription polymerase chain reaction (RT-PCR) was used as the reference standard.
[h=4]RESULTS:[/h] Compared to RT-PCR, Alere[SUP]?[/SUP] iNAT detected 77?8% influenza A positive samples versus 71?4% and 44?4% for the Quidel Sofia[SUP]?[/SUP] Influenza A+B FIA and BinaxNOW[SUP]?[/SUP] Influenza A & B ICT assay, respectively. For influenza B, Alere[SUP]?[/SUP] iNAT detected 75% of those positive by RT-PCR, versus 33?3% and 25?0% for Sofia[SUP]?[/SUP] and BinaxNOW[SUP]?[/SUP] , respectively. The specificity of Alere[SUP]?[/SUP] iNAT was 100% for influenza A and 99% for influenza B.
[h=4]CONCLUSIONS:[/h] Alere[SUP]?[/SUP] i Influenza A&B is a promising new rapid influenza diagnostic assay with potential point-of-care applications.
? 2015 The Authors. Influenza and Other Respiratory Viruses Published by John Wiley & Sons Ltd.
[h=4]KEYWORDS:[/h] Influenza; isothermal nucleic acid amplification; rapid diagnostic test(s); sensitivity; specificity
PMID: 25728758 [PubMed - as supplied by publisher]