tetano
Editor, Senior Moderator
Microbiol Immunol. 2012 Jun;56(6):372-7. doi: 10.1111/j.1348-0421.2012.00455.x.
Development and diagnostic application/evaluation of pandemic (H1N1) 2009 influenza virus-specific monoclonal antibodies.
Kim WS, Lee GC, Yoo JH, Kim HY, Yun YP, Chong CK.
Source
College of Pharmacy, Chungbuk National University, Chungbuk 361-763 Water Analysis and Research Center, K-Water, Daejeon 306-711 Department of Laboratory Medicine, National Health Insurance Corporation Ilsan Hospital, Goyang 410-719 Department of Biochemistry, College of Natural Sciences, Chungbuk National University, Cheongju, Chungbuk 361-763, Korea.
Abstract
We prepared mAb specific to the H1N1 2009 virus (H1N1 2009) to facilitate development of an RDT with enhanced sensitivity and specificity. Among these antibodies, we identified two clones-hybridomas 1H7E1 and 3A3H7-that specifically bound to H1N1 2009 (non-seasonal) and were very suitable for application to a diagnostic kit. The affinity constants (K(a) ) of 1H7E1 and 3A3H7 were 1.10 ? 10(10) and 2.35 ? 10(10) , respectively. To identify the antibodies, we performed ELISA and immunoblot analyses and found that 1H7E1 recognized a conformational epitope of HA while 3A3H7 recognized a linear epitope. In clinical evaluations using specimens from 215 patients, a lateral flow rapid testing kit comprising these mAb showed a sensitivity of 81.5% (75/92) and a specificity of 96.7% (119/123). Results using the RDT kit were well correlated with conventional RT-PCR methods as commonly and commercially used. Based on our findings, we believe that use of these mAb with a rapid evaluation kit could serve as a good diagnostic tool for H1N1 2009.
? 2012 The Societies and Blackwell Publishing Asia Pty Ltd.
PMID:
22686191
[PubMed - in process]
http://www.ncbi.nlm.nih.gov/pubmed/22686191
Development and diagnostic application/evaluation of pandemic (H1N1) 2009 influenza virus-specific monoclonal antibodies.
Kim WS, Lee GC, Yoo JH, Kim HY, Yun YP, Chong CK.
Source
College of Pharmacy, Chungbuk National University, Chungbuk 361-763 Water Analysis and Research Center, K-Water, Daejeon 306-711 Department of Laboratory Medicine, National Health Insurance Corporation Ilsan Hospital, Goyang 410-719 Department of Biochemistry, College of Natural Sciences, Chungbuk National University, Cheongju, Chungbuk 361-763, Korea.
Abstract
We prepared mAb specific to the H1N1 2009 virus (H1N1 2009) to facilitate development of an RDT with enhanced sensitivity and specificity. Among these antibodies, we identified two clones-hybridomas 1H7E1 and 3A3H7-that specifically bound to H1N1 2009 (non-seasonal) and were very suitable for application to a diagnostic kit. The affinity constants (K(a) ) of 1H7E1 and 3A3H7 were 1.10 ? 10(10) and 2.35 ? 10(10) , respectively. To identify the antibodies, we performed ELISA and immunoblot analyses and found that 1H7E1 recognized a conformational epitope of HA while 3A3H7 recognized a linear epitope. In clinical evaluations using specimens from 215 patients, a lateral flow rapid testing kit comprising these mAb showed a sensitivity of 81.5% (75/92) and a specificity of 96.7% (119/123). Results using the RDT kit were well correlated with conventional RT-PCR methods as commonly and commercially used. Based on our findings, we believe that use of these mAb with a rapid evaluation kit could serve as a good diagnostic tool for H1N1 2009.
? 2012 The Societies and Blackwell Publishing Asia Pty Ltd.
PMID:
22686191
[PubMed - in process]
http://www.ncbi.nlm.nih.gov/pubmed/22686191