tetano
Editor, Senior Moderator
Diagn Microbiol Infect Dis. 2018 Apr 18. pii: S0732-8893(18)30119-6. doi: 10.1016/j.diagmicrobio.2018.04.006. [Epub ahead of print]
[h=1]Development and evaluation of a rapid nucleic acid amplification method to detect influenza A and B viruses in human respiratory specimens.[/h]
[h=1][/h] Elf S[SUP]1[/SUP], Auvinen P[SUP]1[/SUP], Jahn L[SUP]2[/SUP], Liikonen K[SUP]1[/SUP], Sj?blom S[SUP]1[/SUP], Saavalainen P[SUP]3[/SUP], M?ki M[SUP]1[/SUP], Eboigbodin KE[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Isothermal nucleic acid amplification methods can potentially shorten the amount of time required to diagnose influenza. We developed and evaluated a novel isothermal nucleic acid amplification method, RT-SIBA to rapidly detect and differentiate between influenza A and B viruses in a single reaction tube. The performance of the RT-SIBA Influenza assay was compared with two established RT-PCR methods. The sensitivities of the RT-SIBA, RealStar RT-PCR, and CDC RT-PCR assays for the detection of influenza A and B viruses in the clinical specimens were 98.8%, 100%, and 89.3%, respectively. All three assays demonstrated a specificity of 100%. The average time to positive result was significantly shorter with the RT-SIBA Influenza assay (<20 min) than with the two RT-PCR assays (>90 min). The method can be run using battery-operated, portable devices with a small footprint and therefore has potential applications in both laboratory and near-patient settings.
[h=4]KEYWORDS:[/h] Amplification; Diagnostics; Influenza; Isothermal; Portable; Virus
PMID: 29778563 DOI: 10.1016/j.diagmicrobio.2018.04.006
[h=1]Development and evaluation of a rapid nucleic acid amplification method to detect influenza A and B viruses in human respiratory specimens.[/h]
[h=1][/h] Elf S[SUP]1[/SUP], Auvinen P[SUP]1[/SUP], Jahn L[SUP]2[/SUP], Liikonen K[SUP]1[/SUP], Sj?blom S[SUP]1[/SUP], Saavalainen P[SUP]3[/SUP], M?ki M[SUP]1[/SUP], Eboigbodin KE[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Isothermal nucleic acid amplification methods can potentially shorten the amount of time required to diagnose influenza. We developed and evaluated a novel isothermal nucleic acid amplification method, RT-SIBA to rapidly detect and differentiate between influenza A and B viruses in a single reaction tube. The performance of the RT-SIBA Influenza assay was compared with two established RT-PCR methods. The sensitivities of the RT-SIBA, RealStar RT-PCR, and CDC RT-PCR assays for the detection of influenza A and B viruses in the clinical specimens were 98.8%, 100%, and 89.3%, respectively. All three assays demonstrated a specificity of 100%. The average time to positive result was significantly shorter with the RT-SIBA Influenza assay (<20 min) than with the two RT-PCR assays (>90 min). The method can be run using battery-operated, portable devices with a small footprint and therefore has potential applications in both laboratory and near-patient settings.
[h=4]KEYWORDS:[/h] Amplification; Diagnostics; Influenza; Isothermal; Portable; Virus
PMID: 29778563 DOI: 10.1016/j.diagmicrobio.2018.04.006