tetano
Editor, Senior Moderator
Biochem Biophys Res Commun. 2015 Jan 2. pii: S0006-291X(14)02281-5. doi: 10.1016/j.bbrc.2014.12.092. [Epub ahead of print]
[h=1]Development of a functional cell-based assay that probes the specific interaction between influenza A virus NP and its packaging signal sequence RNA.[/h] Woo J[SUP]1[/SUP], Yu KL[SUP]1[/SUP], Lee SH[SUP]2[/SUP], You JC[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Although cis-acting packaging signal RNA sequences for the influenza virus NP encoding vRNA have been identified recently though genetic studies, little is known about the interaction between NP and the vRNA packaging signals either in vivo or in vitro. Here, we provide evidence that NP is able to interact specifically with the vRNA packaging sequence RNA within living cells and that the specific RNA binding activity of NP in vivo requires both the N-terminal and central region of the protein. This assay established would be a valuable tool for further detailed studies of the NP-packaging signal RNA interaction in living cells.
Copyright ? 2014 Elsevier Inc. All rights reserved.
[h=4]KEYWORDS:[/h] Cell-based protein?RNA interaction; Influenza; Nucleoprotein; Packaging signal sequence
PMID: 25559349 [PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/25559349
[h=1]Development of a functional cell-based assay that probes the specific interaction between influenza A virus NP and its packaging signal sequence RNA.[/h] Woo J[SUP]1[/SUP], Yu KL[SUP]1[/SUP], Lee SH[SUP]2[/SUP], You JC[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Although cis-acting packaging signal RNA sequences for the influenza virus NP encoding vRNA have been identified recently though genetic studies, little is known about the interaction between NP and the vRNA packaging signals either in vivo or in vitro. Here, we provide evidence that NP is able to interact specifically with the vRNA packaging sequence RNA within living cells and that the specific RNA binding activity of NP in vivo requires both the N-terminal and central region of the protein. This assay established would be a valuable tool for further detailed studies of the NP-packaging signal RNA interaction in living cells.
Copyright ? 2014 Elsevier Inc. All rights reserved.
[h=4]KEYWORDS:[/h] Cell-based protein?RNA interaction; Influenza; Nucleoprotein; Packaging signal sequence
PMID: 25559349 [PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/25559349