tetano
Editor, Senior Moderator
Sensors (Basel). 2012;12(8):10810-9. doi: 10.3390/s120810810. Epub 2012 Aug 6.
Development of a Plastic-Based Microfluidic Immunosensor Chip for Detection of H1N1 Influenza.
Lee KG, Lee TJ, Jeong SW, Choi HW, Heo NS, Park JY, Park TJ, Lee SJ.
Source
Center for Nanobio Integration & Convergence Engineering (NICE), National NanoFab Center, 291 Daehak-ro, Yuseong-gu, Daejeon 305-806, Korea; E-Mails: kglee@nnfc.re.kr (K.G.L.); tjlee@nnfc.re.kr (T.J.L.); swjeong@nnfc.re.kr (S.W.J.); hwchoi@nnfc.re.kr (H.W.C.).
Abstract
Lab-on-a-chip can provide convenient and accurate diagnosis tools. In this paper, a plastic-based microfluidic immunosensor chip for the diagnosis of swine flu (H1N1) was developed by immobilizing hemagglutinin antigen on a gold surface using a genetically engineered polypeptide. A fluorescent dye-labeled antibody (Ab) was used for quantifying the concentration of Ab in the immunosensor chip using a fluorescent technique. For increasing the detection efficiency and reducing the errors, three chambers and three microchannels were designed in one microfluidic chip. This protocol could be applied to the diagnosis of other infectious diseases in a microfluidic device.
PMID:
23112630
[PubMed - in process]
PMCID:
PMC3472858
http://www.ncbi.nlm.nih.gov/pubmed/23112630
Development of a Plastic-Based Microfluidic Immunosensor Chip for Detection of H1N1 Influenza.
Lee KG, Lee TJ, Jeong SW, Choi HW, Heo NS, Park JY, Park TJ, Lee SJ.
Source
Center for Nanobio Integration & Convergence Engineering (NICE), National NanoFab Center, 291 Daehak-ro, Yuseong-gu, Daejeon 305-806, Korea; E-Mails: kglee@nnfc.re.kr (K.G.L.); tjlee@nnfc.re.kr (T.J.L.); swjeong@nnfc.re.kr (S.W.J.); hwchoi@nnfc.re.kr (H.W.C.).
Abstract
Lab-on-a-chip can provide convenient and accurate diagnosis tools. In this paper, a plastic-based microfluidic immunosensor chip for the diagnosis of swine flu (H1N1) was developed by immobilizing hemagglutinin antigen on a gold surface using a genetically engineered polypeptide. A fluorescent dye-labeled antibody (Ab) was used for quantifying the concentration of Ab in the immunosensor chip using a fluorescent technique. For increasing the detection efficiency and reducing the errors, three chambers and three microchannels were designed in one microfluidic chip. This protocol could be applied to the diagnosis of other infectious diseases in a microfluidic device.
PMID:
23112630
[PubMed - in process]
PMCID:
PMC3472858
http://www.ncbi.nlm.nih.gov/pubmed/23112630