tetano
Editor, Senior Moderator
BMC Infect Dis. 2014 Jul 3;14(1):362. [Epub ahead of print]
Development of a sensitive novel diagnostic kit for the highly pathogenic avian influenza A (H5N1) virus.
Tsunetsugu-Yokota Y, Nishimura K, Misawa S, Kobayashi-Ishihara M, Takahashi H, Takayama I, Ohnishi K, Itamura S, Nguyen HL, Le MT, Dang GT, Nguyen LT, Tashiro M, Kageyama T.
Abstract
BACKGROUND:
Sporadic emergence of the highly pathogenic avian influenza (HPAI) H5N1 virus infection in humans is a serious concern because of the potential for a pandemic. Conventional or quantitative RT-PCR is the standard laboratory test to detect viral influenza infections. However, this technology requires well-equipped laboratories and highly trained personnel. A rapid, sensitive, and specific alternative screening method is needed.
METHODS:
By a luminescence-linked enzyme immunoassay, we have developed a H5N1 HPAI virus detection kit using anti-H5 hemagglutinin monoclonal antibodies in combination with the detection of a universal NP antigen of the type A influenza virus. The process takes 15 minutes by use of the fully automated luminescence analyzer, POCube.Resutls: We tested this H5/A kit using 19 clinical specimens from 13 patients stored in Vietnam who were infected with clade 1.1 or clade 2.3.4 H5N1 HPAI virus. Approximately 80% of clinical specimens were H5-positive using the POCube system, whereas only 10% of the H5-positive samples were detected as influenza A-positive by an immunochromatography-based rapid diagnostic kit.
CONCLUSIONS:
This novel H5/A kit using POCube is served as a rapid and sensitive screening test for H5N1 HPAI virus infection in humans.
PMID:
24992826
[PubMed - as supplied by publisher]
Free full text
http://www.ncbi.nlm.nih.gov/pubmed/24992826
Development of a sensitive novel diagnostic kit for the highly pathogenic avian influenza A (H5N1) virus.
Tsunetsugu-Yokota Y, Nishimura K, Misawa S, Kobayashi-Ishihara M, Takahashi H, Takayama I, Ohnishi K, Itamura S, Nguyen HL, Le MT, Dang GT, Nguyen LT, Tashiro M, Kageyama T.
Abstract
BACKGROUND:
Sporadic emergence of the highly pathogenic avian influenza (HPAI) H5N1 virus infection in humans is a serious concern because of the potential for a pandemic. Conventional or quantitative RT-PCR is the standard laboratory test to detect viral influenza infections. However, this technology requires well-equipped laboratories and highly trained personnel. A rapid, sensitive, and specific alternative screening method is needed.
METHODS:
By a luminescence-linked enzyme immunoassay, we have developed a H5N1 HPAI virus detection kit using anti-H5 hemagglutinin monoclonal antibodies in combination with the detection of a universal NP antigen of the type A influenza virus. The process takes 15 minutes by use of the fully automated luminescence analyzer, POCube.Resutls: We tested this H5/A kit using 19 clinical specimens from 13 patients stored in Vietnam who were infected with clade 1.1 or clade 2.3.4 H5N1 HPAI virus. Approximately 80% of clinical specimens were H5-positive using the POCube system, whereas only 10% of the H5-positive samples were detected as influenza A-positive by an immunochromatography-based rapid diagnostic kit.
CONCLUSIONS:
This novel H5/A kit using POCube is served as a rapid and sensitive screening test for H5N1 HPAI virus infection in humans.
PMID:
24992826
[PubMed - as supplied by publisher]
Free full text
http://www.ncbi.nlm.nih.gov/pubmed/24992826