tetano
Editor, Senior Moderator
Osong Public Health Res Perspect. 2013 Dec;4(6):342-6. doi: 10.1016/j.phrp.2013.10.006. Epub 2013 Nov 7.
Development of a Specific and Rapid Diagnostic Method for Detecting Influenza A (H1N1) pdm09 Virus Infection Using Immunochromatographic Assay.
Ji MJ1, Cho BK2, Cho YS2, Choi YJ3, Kwon D4, Shin K4, Lee JY4, Kang C4, Yoon BS5.
Author information
Abstract
OBJECTIVES:
The aim of this study was to develop an immunochromatographic assay (ICA) for the detection of influenza A (H1N1) pdm09 virus infection.
MATERIALS AND METHODS:
Several monoclonal antibodies against influenza A (H1N1) pdm09 virus were generated and an ICA (pdm09-ICA) was developed for the rapid and specific detection of influenza A (H1N1) pdm09 virus infection. The specificity and sensitivity of the developed assay were compared with that of hemagglutination assay and real-time reverse-transcription polymerase chain reaction (rRT-PCR).
RESULTS:
The detection limit was estimated to be 1/2 (8) hemagglutinating unit; the sensitivity and specificity rates of pdm09-ICA were 75.86% (110/145) and 100% (43/43), respectively, compared with rRT-PCR. The cross-reactivity for 20 influenza viruses, including seasonal H1N1 viruses, was found to be negative except for the H1N1 virus (A/Swine/Korea/GC0503/2005).
CONCLUSION:
These results indicate that the proposed method can be easily used for rapid and specific detection of the pdm09 infection. The assay developed in this study would be a useful tool for distinguishing the pdm09 infection from seasonal influenza A and B infections.
KEYWORDS:
immunochromatographic assay, immunochromatography test, influenza, pdm09
PMID:
24524023
[PubMed]
http://www.ncbi.nlm.nih.gov/pubmed/24524023
Development of a Specific and Rapid Diagnostic Method for Detecting Influenza A (H1N1) pdm09 Virus Infection Using Immunochromatographic Assay.
Ji MJ1, Cho BK2, Cho YS2, Choi YJ3, Kwon D4, Shin K4, Lee JY4, Kang C4, Yoon BS5.
Author information
Abstract
OBJECTIVES:
The aim of this study was to develop an immunochromatographic assay (ICA) for the detection of influenza A (H1N1) pdm09 virus infection.
MATERIALS AND METHODS:
Several monoclonal antibodies against influenza A (H1N1) pdm09 virus were generated and an ICA (pdm09-ICA) was developed for the rapid and specific detection of influenza A (H1N1) pdm09 virus infection. The specificity and sensitivity of the developed assay were compared with that of hemagglutination assay and real-time reverse-transcription polymerase chain reaction (rRT-PCR).
RESULTS:
The detection limit was estimated to be 1/2 (8) hemagglutinating unit; the sensitivity and specificity rates of pdm09-ICA were 75.86% (110/145) and 100% (43/43), respectively, compared with rRT-PCR. The cross-reactivity for 20 influenza viruses, including seasonal H1N1 viruses, was found to be negative except for the H1N1 virus (A/Swine/Korea/GC0503/2005).
CONCLUSION:
These results indicate that the proposed method can be easily used for rapid and specific detection of the pdm09 infection. The assay developed in this study would be a useful tool for distinguishing the pdm09 infection from seasonal influenza A and B infections.
KEYWORDS:
immunochromatographic assay, immunochromatography test, influenza, pdm09
PMID:
24524023
[PubMed]
http://www.ncbi.nlm.nih.gov/pubmed/24524023