tetano
Editor, Senior Moderator
Virology. 2018 Oct 26;526:125-137. doi: 10.1016/j.virol.2018.10.005. [Epub ahead of print]
[h=1]Development of a universal influenza vaccine using hemagglutinin stem protein produced from Pichia pastoris.[/h] Wang SC[SUP]1[/SUP], Liao HY[SUP]2[/SUP], Zhang JY[SUP]2[/SUP], Cheng TR[SUP]3[/SUP], Wong CH[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The development of a universal influenza vaccine has become a major effort to combat the high mutation rate of influenza. To explore the use of the highly conserved stem region of hemagglutinin (HA) as a universal vaccine, we produced HA-stem-based protein using yeast expression systems. The glycosylation effects on the immunogenicity and protection activities were investigated. The yield of the A/Brisbane/59/2007 HA stem produced from Pichia pastoris reached 100 mg/l. The immunogenicity of HA stem proteins in various glycoforms was further investigated and compared. All glycoforms of the HA stem protein can induce cross-reactive antibody responses, antibody-dependent cellular cytotoxicity (ADCC)-mediated protection as well as T-cell responses, with broad protection in mice. The monoglycosylated form of the A/Brisbane/59/2007 HA stem produced in yeast, together with the glycolipid C34 as the adjuvant, can elicit greater ADCC responses, better neutralizing activities against heterologous strains, and broader protection in mice.
[h=4]KEYWORDS:[/h] Fermentation; Hemagglutinin stem; Influenza vaccine; Pichia pastoris
PMID: 30388628 DOI: 10.1016/j.virol.2018.10.005
[h=1]Development of a universal influenza vaccine using hemagglutinin stem protein produced from Pichia pastoris.[/h] Wang SC[SUP]1[/SUP], Liao HY[SUP]2[/SUP], Zhang JY[SUP]2[/SUP], Cheng TR[SUP]3[/SUP], Wong CH[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The development of a universal influenza vaccine has become a major effort to combat the high mutation rate of influenza. To explore the use of the highly conserved stem region of hemagglutinin (HA) as a universal vaccine, we produced HA-stem-based protein using yeast expression systems. The glycosylation effects on the immunogenicity and protection activities were investigated. The yield of the A/Brisbane/59/2007 HA stem produced from Pichia pastoris reached 100 mg/l. The immunogenicity of HA stem proteins in various glycoforms was further investigated and compared. All glycoforms of the HA stem protein can induce cross-reactive antibody responses, antibody-dependent cellular cytotoxicity (ADCC)-mediated protection as well as T-cell responses, with broad protection in mice. The monoglycosylated form of the A/Brisbane/59/2007 HA stem produced in yeast, together with the glycolipid C34 as the adjuvant, can elicit greater ADCC responses, better neutralizing activities against heterologous strains, and broader protection in mice.
[h=4]KEYWORDS:[/h] Fermentation; Hemagglutinin stem; Influenza vaccine; Pichia pastoris
PMID: 30388628 DOI: 10.1016/j.virol.2018.10.005