tetano
Editor, Senior Moderator
Clin Lab. 2014;60(5):815-9.
Development of an immunochromatographic kit for rapid detection of human influenza B virus infection.
Zhang P, Duan Y, Zhang D, Zhang S, Li Z, Wang X, Yang P.
Abstract
BACKGROUND:
Type B influenza virus is a major epidemic strain responsible for considerable mortality and morbidity.
METHODS:
A colloidal gold immunochromatographic strip for the rapid detection of human influenza B virus was developed. This test is based on membrane chromatography and uses colloidal gold conjugated with influenza B virus anti-NP monoclonal antibody as the tracer. The assembled test strip was housed in a plastic case.
RESULTS:
The colloid gold strip (CGS) specifically detected all influenza B viruses tested and did not react with other respiratory viruses. Compared with SYBR Green real-time PCR, the sensitivity and specificity of the CGS test was 89.76% and 99.56%, respectively, and the consistency ratio between CGS and real-time PCR was 96.06% in detecting influenza B virus in 710 nasopharyngeal swabs from patients with influenza-like illness in the hospital.
CONCLUSIONS:
The CGS array developed in this study enabled typing of influenza B viruses in human clinical specimens. Thus, together with the advantages of rapid detection and easy operation without requiring specialized personnel and equipment, this technique is a convenient and relatively inexpensive diagnostic tool for large-scale screening of clinical samples.
PMID:
24839825
[PubMed - in process]
http://www.ncbi.nlm.nih.gov/pubmed/24839825
Development of an immunochromatographic kit for rapid detection of human influenza B virus infection.
Zhang P, Duan Y, Zhang D, Zhang S, Li Z, Wang X, Yang P.
Abstract
BACKGROUND:
Type B influenza virus is a major epidemic strain responsible for considerable mortality and morbidity.
METHODS:
A colloidal gold immunochromatographic strip for the rapid detection of human influenza B virus was developed. This test is based on membrane chromatography and uses colloidal gold conjugated with influenza B virus anti-NP monoclonal antibody as the tracer. The assembled test strip was housed in a plastic case.
RESULTS:
The colloid gold strip (CGS) specifically detected all influenza B viruses tested and did not react with other respiratory viruses. Compared with SYBR Green real-time PCR, the sensitivity and specificity of the CGS test was 89.76% and 99.56%, respectively, and the consistency ratio between CGS and real-time PCR was 96.06% in detecting influenza B virus in 710 nasopharyngeal swabs from patients with influenza-like illness in the hospital.
CONCLUSIONS:
The CGS array developed in this study enabled typing of influenza B viruses in human clinical specimens. Thus, together with the advantages of rapid detection and easy operation without requiring specialized personnel and equipment, this technique is a convenient and relatively inexpensive diagnostic tool for large-scale screening of clinical samples.
PMID:
24839825
[PubMed - in process]
http://www.ncbi.nlm.nih.gov/pubmed/24839825