tetano
Editor, Senior Moderator
Bull Exp Biol Med. 2018 Jun 19. doi: 10.1007/s10517-018-4139-1. [Epub ahead of print]
[h=1]Development of Pyrosequencing Assay for Evaluation of Genetic Stability of Vaccine Strains of Live Attenuated Influenza Type B Vaccine.[/h] Stepanova EА[SUP]1[/SUP], Krutikova EV, Kiseleva IV, Vasil'eva VA, Rudenko LG.
[h=3]Author information[/h]
[h=3]Abstract[/h] We developed a pyrosequencing protocol for monitoring of stability of attenuating mutations in the genome of vaccine reassortants based on master donor virus of Russian live attenuated influenza vaccine B/USSR/60/69. The developed protocol allows rapid and accurate assessment of mutations and can be used for analysis of genetic stability of reassortants during vaccine strain development and manufacturing, as well as genetic stability of vaccine isolates of influenza B virus during pre-clinical and clinical trials.
[h=4]KEYWORDS:[/h] influenza B virus; live attenuated influenza vaccine; pyrosequencing
PMID: 29923003 DOI: 10.1007/s10517-018-4139-1
[h=1]Development of Pyrosequencing Assay for Evaluation of Genetic Stability of Vaccine Strains of Live Attenuated Influenza Type B Vaccine.[/h] Stepanova EА[SUP]1[/SUP], Krutikova EV, Kiseleva IV, Vasil'eva VA, Rudenko LG.
[h=3]Author information[/h]
[h=3]Abstract[/h] We developed a pyrosequencing protocol for monitoring of stability of attenuating mutations in the genome of vaccine reassortants based on master donor virus of Russian live attenuated influenza vaccine B/USSR/60/69. The developed protocol allows rapid and accurate assessment of mutations and can be used for analysis of genetic stability of reassortants during vaccine strain development and manufacturing, as well as genetic stability of vaccine isolates of influenza B virus during pre-clinical and clinical trials.
[h=4]KEYWORDS:[/h] influenza B virus; live attenuated influenza vaccine; pyrosequencing
PMID: 29923003 DOI: 10.1007/s10517-018-4139-1