tetano
Editor, Senior Moderator
Diagn Microbiol Infect Dis
. 2024 Apr 4;109(3):116297.
doi: 10.1016/j.diagmicrobio.2024.116297. Online ahead of print. Evaluation of the SARS-CoV-2 RNA detection reagent LAMPdirect Genelyzer KIT using nasopharyngeal swab and saliva samples
Junko S Takeuchi[SUP] 1 [/SUP], Kento Fukano[SUP] 2 [/SUP], Yumiko Kito[SUP] 3 [/SUP], Kei Yamamoto[SUP] 4 [/SUP], Moto Kimura[SUP] 5 [/SUP]
Affiliations
The LAMPdirect Genelyzer KIT allows for the detection of SARS-CoV-2 RNA in saliva samples with a loop-mediated isothermal amplification (LAMP) method and generates results within 20 min. It has been approved by the Pharmaceuticals and Medical Devices Agency in Japan. In this study, the performance of the LAMPdirect Genelyzer KIT was compared with that of the RT-qPCR reference method using 50 nasopharyngeal swabs and 100 saliva samples. In addition, we evaluated the applicability of an alternative reverse transcriptase and the effects of an inactivation buffer. The total agreement rates were 80.0 % and 82.0 % for nasopharyngeal and saliva samples, respectively. When considering samples at the detection limit (50 copies/reaction) that increases the chance of transmission between humans, the total agreement rates were 100% and 94.1% for nasopharyngeal and saliva samples, respectively. The LAMP method is simple, fast, and inexpensive, making it useful for small medical institutions or rural areas.
Keywords: COVID-19; Clinical sample; In vitro diagnostics (IVD); Loop-mediated isothermal amplification (LAMP); RT-qPCR; SARS-CoV-2.
. 2024 Apr 4;109(3):116297.
doi: 10.1016/j.diagmicrobio.2024.116297. Online ahead of print. Evaluation of the SARS-CoV-2 RNA detection reagent LAMPdirect Genelyzer KIT using nasopharyngeal swab and saliva samples
Junko S Takeuchi[SUP] 1 [/SUP], Kento Fukano[SUP] 2 [/SUP], Yumiko Kito[SUP] 3 [/SUP], Kei Yamamoto[SUP] 4 [/SUP], Moto Kimura[SUP] 5 [/SUP]
Affiliations
- PMID: 38604076
- DOI: 10.1016/j.diagmicrobio.2024.116297
The LAMPdirect Genelyzer KIT allows for the detection of SARS-CoV-2 RNA in saliva samples with a loop-mediated isothermal amplification (LAMP) method and generates results within 20 min. It has been approved by the Pharmaceuticals and Medical Devices Agency in Japan. In this study, the performance of the LAMPdirect Genelyzer KIT was compared with that of the RT-qPCR reference method using 50 nasopharyngeal swabs and 100 saliva samples. In addition, we evaluated the applicability of an alternative reverse transcriptase and the effects of an inactivation buffer. The total agreement rates were 80.0 % and 82.0 % for nasopharyngeal and saliva samples, respectively. When considering samples at the detection limit (50 copies/reaction) that increases the chance of transmission between humans, the total agreement rates were 100% and 94.1% for nasopharyngeal and saliva samples, respectively. The LAMP method is simple, fast, and inexpensive, making it useful for small medical institutions or rural areas.
Keywords: COVID-19; Clinical sample; In vitro diagnostics (IVD); Loop-mediated isothermal amplification (LAMP); RT-qPCR; SARS-CoV-2.