tetano
Editor, Senior Moderator
Diagn Pathol
. 2022 Feb 17;17(1):31.
doi: 10.1186/s13000-022-01207-6.
Morphologically, immunohistochemically and PCR proven lymphocytic viral peri-, endo-, myocarditis in patients with fatal COVID-19
Evgeniya Kogan[SUP] 1 [/SUP], Yuriy Berezovskiy[SUP] 2 [/SUP], Olga Blagova[SUP] 3 [/SUP], Anna Kukleva[SUP] 1 [/SUP], Lyudmila Semyonova[SUP] 2 [/SUP], Evgeniy Gretsov[SUP] 2 [/SUP], Atadzhan Ergeshov[SUP] 2 [/SUP]
Affiliations
Abstract
Background: Despite a reported cardiac injury in patients with new coronavirus infection, the possibility and specifics of genuine viral myocarditis in COVID-19 remains not fully clear.
Purpose: To study the presence of SARS-CoV-2 in the myocardium and the morphological properties of myocarditis in patients with severe coronavirus infection (COVID-19).
Methods: Autopsy data of eight elderly patients (75.6 ± 7.4 years), four male and four female, with severe new coronavirus infection were studied. The lifetime diagnosis of COVID-19 is based on a positive result of the PCR study. The inclusion criterion was the presence of morphological signs of myocarditis according to the Dallas criteria. A standard histological examination included staining by hematoxylin and eosin, toluidin blue and Van Gieson. An immunohistochemical study was performed using antibodies to CD3, CD 68, CD20, perforin, toll-like receptor (TLR) types 4 and 9. PCR in real-time was performed to determine the viral RNA in the myocardium.
Results: All patients had severe bilateral viral pneumonia. In all cases, myocarditis was not clinically diagnosed. Morphological examination of the heart found signs of active lymphocytic myocarditis. PCR identified the SARS-Cov2 RNA in all cases. There were also signs of destructive coronaritis in all cases, thrombovasculitis, lymphocytic pericarditis (in 3 cases) and endocarditis (in 2 cases). The absence of neutrophils confirms the aseptic nature of inflammation. An immunohistochemical study showed the CD3-positive T lymphocytes in the infiltrates. Increased expression of TLR type 4 and less 9 was also detected.
Conclusion: Morphological and immunohistochemical evidence of myocarditis in COVID-19 was presented. Lymphocytic infiltrations and positive PCR confirm the viral nature of inflammation. Myocarditis in COVID-19 is also characterized by coronaritis with microvascular thrombosis and associated with lymphocytic endo- and pericarditis.
Keywords: COVID-19; Coronavirus; Endocarditis; Morphological and immunohistochemical study; Myocarditis; PCR; Pericarditis; Toll-like receptors.
. 2022 Feb 17;17(1):31.
doi: 10.1186/s13000-022-01207-6.
Morphologically, immunohistochemically and PCR proven lymphocytic viral peri-, endo-, myocarditis in patients with fatal COVID-19
Evgeniya Kogan[SUP] 1 [/SUP], Yuriy Berezovskiy[SUP] 2 [/SUP], Olga Blagova[SUP] 3 [/SUP], Anna Kukleva[SUP] 1 [/SUP], Lyudmila Semyonova[SUP] 2 [/SUP], Evgeniy Gretsov[SUP] 2 [/SUP], Atadzhan Ergeshov[SUP] 2 [/SUP]
Affiliations
- PMID: 35177093
- DOI: 10.1186/s13000-022-01207-6
Abstract
Background: Despite a reported cardiac injury in patients with new coronavirus infection, the possibility and specifics of genuine viral myocarditis in COVID-19 remains not fully clear.
Purpose: To study the presence of SARS-CoV-2 in the myocardium and the morphological properties of myocarditis in patients with severe coronavirus infection (COVID-19).
Methods: Autopsy data of eight elderly patients (75.6 ± 7.4 years), four male and four female, with severe new coronavirus infection were studied. The lifetime diagnosis of COVID-19 is based on a positive result of the PCR study. The inclusion criterion was the presence of morphological signs of myocarditis according to the Dallas criteria. A standard histological examination included staining by hematoxylin and eosin, toluidin blue and Van Gieson. An immunohistochemical study was performed using antibodies to CD3, CD 68, CD20, perforin, toll-like receptor (TLR) types 4 and 9. PCR in real-time was performed to determine the viral RNA in the myocardium.
Results: All patients had severe bilateral viral pneumonia. In all cases, myocarditis was not clinically diagnosed. Morphological examination of the heart found signs of active lymphocytic myocarditis. PCR identified the SARS-Cov2 RNA in all cases. There were also signs of destructive coronaritis in all cases, thrombovasculitis, lymphocytic pericarditis (in 3 cases) and endocarditis (in 2 cases). The absence of neutrophils confirms the aseptic nature of inflammation. An immunohistochemical study showed the CD3-positive T lymphocytes in the infiltrates. Increased expression of TLR type 4 and less 9 was also detected.
Conclusion: Morphological and immunohistochemical evidence of myocarditis in COVID-19 was presented. Lymphocytic infiltrations and positive PCR confirm the viral nature of inflammation. Myocarditis in COVID-19 is also characterized by coronaritis with microvascular thrombosis and associated with lymphocytic endo- and pericarditis.
Keywords: COVID-19; Coronavirus; Endocarditis; Morphological and immunohistochemical study; Myocarditis; PCR; Pericarditis; Toll-like receptors.