tetano
Editor, Senior Moderator
Electrophoresis. 2017 Jul 17. doi: 10.1002/elps.201700112. [Epub ahead of print]
[h=1]Discriminant Biomarkers of Acute Respiratory Distress Syndrome associated To H1n1 Influenza identified by metabolomics Hplc-Qtof-Ms/Ms platform.[/h] Ferrarini A[SUP]1[/SUP], Righetti L[SUP]1,[/SUP][SUP]2[/SUP], Mart?nez MP[SUP]1[/SUP], Fern?ndez M[SUP]1[/SUP], Mastrangelo A[SUP]1[/SUP], Horcajada JP[SUP]3[/SUP], Betbes? A[SUP]4[/SUP], Esteban A[SUP]5,[/SUP][SUP]6[/SUP], Ord??ez J[SUP]4[/SUP], de Gea J[SUP]3[/SUP], Cabello JR[SUP]6,[/SUP][SUP]7[/SUP], Pellati F[SUP]2[/SUP], Lorente JA[SUP]5,[/SUP][SUP]6[/SUP], Nin N[SUP]8,[/SUP][SUP]9[/SUP], Rup?rez FJ[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Acute Respiratory Distress Syndrome (ARDS) is a serious complication of influenza A (H1N1) virus infection. Its pathogenesis is unknown, and biomarkers are lacking. Untargeted metabolomics allows the analysis of the whole metabolome in a biological compartment, identifying patterns associated with specific conditions. We hypothesized that LC-MS could help identify discriminant metabolites able to define the metabolic alterations occurring in patients with influenza A (H1N1) virus infection that developed ARDS. Serum samples from patients diagnosed with 2009 influenza A (H1N1) virus infection with (n = 25) or without (n = 32) ARDS were obtained on the day of hospital admission and analyzed by LC-MS/MS. Metabolite identification was determined by MS/MS analysis and analysis of standards. The specificity of the patterns identified was confirmed in patients without 2009 influenza A(H1N1) virus pneumonia (15 without and 17 with ARDS). Twenty-three candidate biomarkers were found to be significantly different between the two groups, including lysophospholipids and sphingolipids related to inflammation; bile acids, tryptophan metabolites, and thyroxine, related to the metabolism of the gut microflora. Confirmation results demonstrated the specificity of major alterations occurring in ARDS patients with influenza A (H1N1) virus infection. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
[h=4]KEYWORDS:[/h] ARDS; Biomarkers; Influenza A (H1N1) Virus Infection; Pathogenesis; Untargeted Metabolomics
PMID: 28714069 DOI: 10.1002/elps.201700112
[h=1]Discriminant Biomarkers of Acute Respiratory Distress Syndrome associated To H1n1 Influenza identified by metabolomics Hplc-Qtof-Ms/Ms platform.[/h] Ferrarini A[SUP]1[/SUP], Righetti L[SUP]1,[/SUP][SUP]2[/SUP], Mart?nez MP[SUP]1[/SUP], Fern?ndez M[SUP]1[/SUP], Mastrangelo A[SUP]1[/SUP], Horcajada JP[SUP]3[/SUP], Betbes? A[SUP]4[/SUP], Esteban A[SUP]5,[/SUP][SUP]6[/SUP], Ord??ez J[SUP]4[/SUP], de Gea J[SUP]3[/SUP], Cabello JR[SUP]6,[/SUP][SUP]7[/SUP], Pellati F[SUP]2[/SUP], Lorente JA[SUP]5,[/SUP][SUP]6[/SUP], Nin N[SUP]8,[/SUP][SUP]9[/SUP], Rup?rez FJ[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Acute Respiratory Distress Syndrome (ARDS) is a serious complication of influenza A (H1N1) virus infection. Its pathogenesis is unknown, and biomarkers are lacking. Untargeted metabolomics allows the analysis of the whole metabolome in a biological compartment, identifying patterns associated with specific conditions. We hypothesized that LC-MS could help identify discriminant metabolites able to define the metabolic alterations occurring in patients with influenza A (H1N1) virus infection that developed ARDS. Serum samples from patients diagnosed with 2009 influenza A (H1N1) virus infection with (n = 25) or without (n = 32) ARDS were obtained on the day of hospital admission and analyzed by LC-MS/MS. Metabolite identification was determined by MS/MS analysis and analysis of standards. The specificity of the patterns identified was confirmed in patients without 2009 influenza A(H1N1) virus pneumonia (15 without and 17 with ARDS). Twenty-three candidate biomarkers were found to be significantly different between the two groups, including lysophospholipids and sphingolipids related to inflammation; bile acids, tryptophan metabolites, and thyroxine, related to the metabolism of the gut microflora. Confirmation results demonstrated the specificity of major alterations occurring in ARDS patients with influenza A (H1N1) virus infection. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
[h=4]KEYWORDS:[/h] ARDS; Biomarkers; Influenza A (H1N1) Virus Infection; Pathogenesis; Untargeted Metabolomics
PMID: 28714069 DOI: 10.1002/elps.201700112