tetano
Editor, Senior Moderator
Immune Netw. 2017 Oct;17(5):326-342. doi: 10.4110/in.2017.17.5.326. Epub 2017 Oct 19.
[h=1]Distinct Effects of Monophosphoryl Lipid A, Oligodeoxynucleotide CpG, and Combination Adjuvants on Modulating Innate and Adaptive Immune Responses to Influenza Vaccination.[/h] Ko EJ[SUP]1,[/SUP][SUP]2[/SUP], Lee YT[SUP]2[/SUP], Lee Y[SUP]2[/SUP], Kim KH[SUP]2[/SUP], Kang SM[SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Monophosphoryl lipid A (MPL) and oligodeoxynucleotide CpG are toll-like receptor (TLR) 4 and 9 agonist, respectively. Here, we investigated the effects of MPL, CpG, and combination adjuvants on stimulating in vitro dendritic cells (DCs), in vivo innate and adaptive immune responses, and protective efficacy of influenza vaccination. Combination of MPL and CpG was found to exhibit distinct effects on stimulating DCs in vitro to secrete IL-12p70 and tumor necrosis factor (TNF)-α and proliferate allogeneic CD8 T cells. Prime immunization of mice with inactivated split influenza vaccine in the presence of low dose MPL+CpG adjuvants increased the induction of virus-specific IgG and IgG2a isotype antibodies. MPL and CpG adjuvants contribute to improving the efficacy of prime influenza vaccination against lethal influenza challenge as determined by body weight monitoring, lung function, viral titers, and histology. A combination of MPL and CpG adjuvants was effective in improving vaccine efficacy as well as in reducing inflammatory immune responses locally and in inducing cellular immune responses upon lethal influenza virus challenge. This study demonstrates unique adjuvant effects of MPL, CpG, and combination adjuvants on modulating innate and adaptive immune responses to influenza prime vaccination.
[h=4]KEYWORDS:[/h] Adjuvant; Influenza vaccine; Monophosphoryl lipid A; Oligonucleotide CpG
PMID: 29093654 PMCID: PMC5662782 DOI: 10.4110/in.2017.17.5.326
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[h=1]Distinct Effects of Monophosphoryl Lipid A, Oligodeoxynucleotide CpG, and Combination Adjuvants on Modulating Innate and Adaptive Immune Responses to Influenza Vaccination.[/h] Ko EJ[SUP]1,[/SUP][SUP]2[/SUP], Lee YT[SUP]2[/SUP], Lee Y[SUP]2[/SUP], Kim KH[SUP]2[/SUP], Kang SM[SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Monophosphoryl lipid A (MPL) and oligodeoxynucleotide CpG are toll-like receptor (TLR) 4 and 9 agonist, respectively. Here, we investigated the effects of MPL, CpG, and combination adjuvants on stimulating in vitro dendritic cells (DCs), in vivo innate and adaptive immune responses, and protective efficacy of influenza vaccination. Combination of MPL and CpG was found to exhibit distinct effects on stimulating DCs in vitro to secrete IL-12p70 and tumor necrosis factor (TNF)-α and proliferate allogeneic CD8 T cells. Prime immunization of mice with inactivated split influenza vaccine in the presence of low dose MPL+CpG adjuvants increased the induction of virus-specific IgG and IgG2a isotype antibodies. MPL and CpG adjuvants contribute to improving the efficacy of prime influenza vaccination against lethal influenza challenge as determined by body weight monitoring, lung function, viral titers, and histology. A combination of MPL and CpG adjuvants was effective in improving vaccine efficacy as well as in reducing inflammatory immune responses locally and in inducing cellular immune responses upon lethal influenza virus challenge. This study demonstrates unique adjuvant effects of MPL, CpG, and combination adjuvants on modulating innate and adaptive immune responses to influenza prime vaccination.
[h=4]KEYWORDS:[/h] Adjuvant; Influenza vaccine; Monophosphoryl lipid A; Oligonucleotide CpG
PMID: 29093654 PMCID: PMC5662782 DOI: 10.4110/in.2017.17.5.326
Free full text