tetano
Editor, Senior Moderator
J Infect Dis. 2014 Oct 21. pii: jiu580. [Epub ahead of print]
Distinct patterns of B-cell activation and priming by natural influenza infection versus inactivated influenza vaccination.
He XS1, Holmes TH2, Sanyal M1, Albrecht RA3, Garc?a-Sastre A4, Dekker CL5, Davis MM6, Greenberg HB7.
Author information
Abstract
BACKGROUND:
The human B-cell response to natural influenza infection has not been extensively investigated at the polyclonal level.
METHODS:
The overall B-cell response of patients acutely infected with the 2009 pandemic influenza A(H1N1)pdm09 virus (pH1N1) was analyzed by determining the acute reactivity of plasmablast-derived polyclonal antibodies (PPAb) to influenza proteins. Recipients of inactivated influenza vaccine containing the same pH1N1 strain were studied for comparison.
RESULTS:
During acute infection robust plasmablast responses to the infecting virus were detected, characterized by a greater PPAb reactivity to the conserved influenza nuclear protein and to heterovariant and heterosubtypic hemagglutinins in comparison to responses to the inactivated pH1N1 vaccine. In the pH1N1 vaccinees, the presence of baseline serum neutralizing antibodies against pH1N1, suggesting previous exposure to natural pH1N1 infection, did not affect the plasmablast response to vaccination; whereas repeated immunization with inactivated pH1N1 vaccine resulted in significantly reduced vaccine-specific and cross-reactive PPAb responses.
CONCLUSIONS:
Natural pH1N1 infection and inactivated pH1N1 vaccination result in very distinct patterns of B-cell activation and priming. These differences are likely to be associated with differences in protective immunity, especially cross-protection against heterovariant and heterosubtypic influenza strains.
? The Author 2014. Published by Oxford University Press on behalf of the Infectious Diseases Society of America. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.
PMID:
25336731
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/25336731
Distinct patterns of B-cell activation and priming by natural influenza infection versus inactivated influenza vaccination.
He XS1, Holmes TH2, Sanyal M1, Albrecht RA3, Garc?a-Sastre A4, Dekker CL5, Davis MM6, Greenberg HB7.
Author information
Abstract
BACKGROUND:
The human B-cell response to natural influenza infection has not been extensively investigated at the polyclonal level.
METHODS:
The overall B-cell response of patients acutely infected with the 2009 pandemic influenza A(H1N1)pdm09 virus (pH1N1) was analyzed by determining the acute reactivity of plasmablast-derived polyclonal antibodies (PPAb) to influenza proteins. Recipients of inactivated influenza vaccine containing the same pH1N1 strain were studied for comparison.
RESULTS:
During acute infection robust plasmablast responses to the infecting virus were detected, characterized by a greater PPAb reactivity to the conserved influenza nuclear protein and to heterovariant and heterosubtypic hemagglutinins in comparison to responses to the inactivated pH1N1 vaccine. In the pH1N1 vaccinees, the presence of baseline serum neutralizing antibodies against pH1N1, suggesting previous exposure to natural pH1N1 infection, did not affect the plasmablast response to vaccination; whereas repeated immunization with inactivated pH1N1 vaccine resulted in significantly reduced vaccine-specific and cross-reactive PPAb responses.
CONCLUSIONS:
Natural pH1N1 infection and inactivated pH1N1 vaccination result in very distinct patterns of B-cell activation and priming. These differences are likely to be associated with differences in protective immunity, especially cross-protection against heterovariant and heterosubtypic influenza strains.
? The Author 2014. Published by Oxford University Press on behalf of the Infectious Diseases Society of America. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.
PMID:
25336731
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/25336731