tetano
Editor, Senior Moderator
Adv Healthc Mater. 2019 Dec 15:e1901176. doi: 10.1002/adhm.201901176. [Epub ahead of print] [h=1]Double-Layered M2e-NA Protein Nanoparticle Immunization Induces Broad Cross-Protection against Different Influenza Viruses in Mice.[/h]
Wang Y[SUP]1[/SUP], Deng L[SUP]1[/SUP], Gonzalez GX[SUP]1[/SUP], Luthra L[SUP]1[/SUP], Dong C[SUP]1[/SUP], Ma Y[SUP]1[/SUP], Zou J[SUP]1[/SUP], Kang SM[SUP]1[/SUP], Wang BZ[SUP]1[/SUP].
[h=3]Author information[/h] 1 Center for Inflammation, Immunity & Infection, Georgia State University Institute for Biomedical Sciences, 100 Piedmont Ave NE, Atlanta, GA, 30303, USA.
[h=3]Abstract[/h] The development of a universal influenza vaccine is an ideal strategy to eliminate public health threats from influenza epidemics and pandemics. This ultimate goal is restricted by the low immunogenicity of conserved influenza epitopes. Layered protein nanoparticles composed of well-designed conserved influenza structures have shown improved immunogenicity with new physical and biochemical features. Herein, structure-stabilized influenza matrix protein 2 ectodomain (M2e) and M2e-neuraminidase fusion (M2e-NA) recombinant proteins are generated and M2e protein nanoparticles and double-layered M2e-NA protein nanoparticles are produced by ethanol desolvation and chemical crosslinking. Immunizations with these protein nanoparticles induce immune protection against different viruses of homologous and heterosubtypic NA in mice. Double-layered M2e-NA protein nanoparticles induce higher levels of humoral and cellular responses compared with their comprising protein mixture or M2e nanoparticles. Strong cytotoxic T cell responses are induced in the layered M2e-NA protein nanoparticle groups. Antibody responses contribute to the heterosubtypic NA immune protection. The protective immunity is long lasting. These results demonstrate that double-layered protein nanoparticles containing structure-stabilized M2e and NA can be developed into a universal influenza vaccine or a synergistic component of such vaccines. Layered protein nanoparticles can be a general vaccine platform for different pathogens.
? 2019 The Authors. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
[h=4]KEYWORDS:[/h] broadly reactive immunity; immune cross-protection; layered protein nanoparticles; universal influenza vaccines
PMID: 31840437 DOI: 10.1002/adhm.201901176
Wang Y[SUP]1[/SUP], Deng L[SUP]1[/SUP], Gonzalez GX[SUP]1[/SUP], Luthra L[SUP]1[/SUP], Dong C[SUP]1[/SUP], Ma Y[SUP]1[/SUP], Zou J[SUP]1[/SUP], Kang SM[SUP]1[/SUP], Wang BZ[SUP]1[/SUP].
[h=3]Author information[/h] 1 Center for Inflammation, Immunity & Infection, Georgia State University Institute for Biomedical Sciences, 100 Piedmont Ave NE, Atlanta, GA, 30303, USA.
[h=3]Abstract[/h] The development of a universal influenza vaccine is an ideal strategy to eliminate public health threats from influenza epidemics and pandemics. This ultimate goal is restricted by the low immunogenicity of conserved influenza epitopes. Layered protein nanoparticles composed of well-designed conserved influenza structures have shown improved immunogenicity with new physical and biochemical features. Herein, structure-stabilized influenza matrix protein 2 ectodomain (M2e) and M2e-neuraminidase fusion (M2e-NA) recombinant proteins are generated and M2e protein nanoparticles and double-layered M2e-NA protein nanoparticles are produced by ethanol desolvation and chemical crosslinking. Immunizations with these protein nanoparticles induce immune protection against different viruses of homologous and heterosubtypic NA in mice. Double-layered M2e-NA protein nanoparticles induce higher levels of humoral and cellular responses compared with their comprising protein mixture or M2e nanoparticles. Strong cytotoxic T cell responses are induced in the layered M2e-NA protein nanoparticle groups. Antibody responses contribute to the heterosubtypic NA immune protection. The protective immunity is long lasting. These results demonstrate that double-layered protein nanoparticles containing structure-stabilized M2e and NA can be developed into a universal influenza vaccine or a synergistic component of such vaccines. Layered protein nanoparticles can be a general vaccine platform for different pathogens.
? 2019 The Authors. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
[h=4]KEYWORDS:[/h] broadly reactive immunity; immune cross-protection; layered protein nanoparticles; universal influenza vaccines
PMID: 31840437 DOI: 10.1002/adhm.201901176