tetano
Editor, Senior Moderator
Drug Test Anal
. 2020 Oct 29.
doi: 10.1002/dta.2946. Online ahead of print.
Fully automated dried blood spot sample handling and extraction for serological testing of human anti-SARS-COV-2 antibodies
Stefan Gaugler[SUP] 1 [/SUP], Pierre-Edouard Sotas[SUP] 2 [/SUP], Katharina Blum[SUP] 1 [/SUP], Marc Luginb?hl[SUP] 1 [/SUP]
Affiliations
Abstract
At the beginning of 2020, an outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) reached pandemic dimensions. Throughout the event diagnostic tests function as an essential tool for understanding, mitigating and implement strategies to curb and receduce infections. Here, we present a novel method for the fully automated dried blood spot (DBS) sample handling and extraction for serological testing of human IgG antibodies against SARS-CoV-2 using a commercial ELISA testing kit. This proof-of-principle pilot study successfully demonstrates the recovery of antibodies in their intact form from DBS using automated, direct sample elution within 100 μL of extraction buffer. The use of minimally invasive DBS sampling provides an alternative to existing analytical procedures such as sampling by venipuncture or nasal swabs. Due to the ease of DBS collection, no third party need be involved, making at-home sampling possible(e.g. during quarantine).
. 2020 Oct 29.
doi: 10.1002/dta.2946. Online ahead of print.
Fully automated dried blood spot sample handling and extraction for serological testing of human anti-SARS-COV-2 antibodies
Stefan Gaugler[SUP] 1 [/SUP], Pierre-Edouard Sotas[SUP] 2 [/SUP], Katharina Blum[SUP] 1 [/SUP], Marc Luginb?hl[SUP] 1 [/SUP]
Affiliations
- PMID: 33119946
- DOI: 10.1002/dta.2946
Abstract
At the beginning of 2020, an outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) reached pandemic dimensions. Throughout the event diagnostic tests function as an essential tool for understanding, mitigating and implement strategies to curb and receduce infections. Here, we present a novel method for the fully automated dried blood spot (DBS) sample handling and extraction for serological testing of human IgG antibodies against SARS-CoV-2 using a commercial ELISA testing kit. This proof-of-principle pilot study successfully demonstrates the recovery of antibodies in their intact form from DBS using automated, direct sample elution within 100 μL of extraction buffer. The use of minimally invasive DBS sampling provides an alternative to existing analytical procedures such as sampling by venipuncture or nasal swabs. Due to the ease of DBS collection, no third party need be involved, making at-home sampling possible(e.g. during quarantine).