tetano
Editor, Senior Moderator
Microbiol Immunol. 2018 Mar 25. doi: 10.1111/1348-0421.12588. [Epub ahead of print]
[h=1]Dynamic expression analysis of chemokine and cytokine genes response to H5N1 and H9N2 avian influenza viruses in DF-1 cells.[/h] Luo C[SUP]1[/SUP], Liu J[SUP]1[/SUP], Qi W[SUP]1[/SUP], Ren X[SUP]1[/SUP], Lu R[SUP]1[/SUP], Liao M[SUP]1[/SUP], Ning Z[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] H5N1 and H9N2 are the most important causes of avian influenza in China. Chemokines and cytokines play an important role in inflammatory response which is clearly different between H5N1 and H9N2 infection. To investigate whether chemokines and cytokines differentially regulated following H5N1 and H9N2 AIVs infection, dynamic expression of chemokines and cytokines including IL8L1, IL8L2, CX3CL1, CCL5, CCL20, K203, SCYA4, XLC1, CCLi10, CCL19, IFN-α, IFN-β, IL-1β, IL-6 and TNF-α, were analyzed by real-time quantitative RT-PCR in DF-1 cells. The results showed that IL8L1, IL8L2, CX3CL1, CCL5, CCL20, K203, SCYA4, IFN-α, IFN-β, IL-1β, IL-6, and TNF-α significantly increased after whether H5N1 or H9N2 AIV infected, while the expression of XCL1, CCLi10 and CCL19 were not detected. H9N2 AIV infection showed a much strong chemokines response than H5N1 while cytokines showed opposite results. K203 is a constant chemotactic factor independent of subtype of AIVs and infectious dose. CCL20 and IL-1β are constant regardless of the infectious dose but depend on the subtype of AIVs. Chemotactic factors IL8L1, IL8L2 and CCL5 are dependent both on subtype of AIVs and infectious dose. K203, CX3CL1, SCYA4, CCL20, IFN-α, IL-1β and TNF-α are the specific for response to H5N1 AIV infection while K203, CCL20, IFN-β, IL-1β and IL-6 for H9N2. The results will provide basic data for explaining inflammation differences and phenotype of histopathological changes caused by H5N1 and H9N2 and add new information on the role of chemokines and cytokines in viral virulence of AIVs.
[h=4]KEYWORDS:[/h] Avian influenza virus; Chemokines; Cytokines; H5N1; H9N2
PMID: 29577370 DOI: 10.1111/1348-0421.12588
[h=1]Dynamic expression analysis of chemokine and cytokine genes response to H5N1 and H9N2 avian influenza viruses in DF-1 cells.[/h] Luo C[SUP]1[/SUP], Liu J[SUP]1[/SUP], Qi W[SUP]1[/SUP], Ren X[SUP]1[/SUP], Lu R[SUP]1[/SUP], Liao M[SUP]1[/SUP], Ning Z[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] H5N1 and H9N2 are the most important causes of avian influenza in China. Chemokines and cytokines play an important role in inflammatory response which is clearly different between H5N1 and H9N2 infection. To investigate whether chemokines and cytokines differentially regulated following H5N1 and H9N2 AIVs infection, dynamic expression of chemokines and cytokines including IL8L1, IL8L2, CX3CL1, CCL5, CCL20, K203, SCYA4, XLC1, CCLi10, CCL19, IFN-α, IFN-β, IL-1β, IL-6 and TNF-α, were analyzed by real-time quantitative RT-PCR in DF-1 cells. The results showed that IL8L1, IL8L2, CX3CL1, CCL5, CCL20, K203, SCYA4, IFN-α, IFN-β, IL-1β, IL-6, and TNF-α significantly increased after whether H5N1 or H9N2 AIV infected, while the expression of XCL1, CCLi10 and CCL19 were not detected. H9N2 AIV infection showed a much strong chemokines response than H5N1 while cytokines showed opposite results. K203 is a constant chemotactic factor independent of subtype of AIVs and infectious dose. CCL20 and IL-1β are constant regardless of the infectious dose but depend on the subtype of AIVs. Chemotactic factors IL8L1, IL8L2 and CCL5 are dependent both on subtype of AIVs and infectious dose. K203, CX3CL1, SCYA4, CCL20, IFN-α, IL-1β and TNF-α are the specific for response to H5N1 AIV infection while K203, CCL20, IFN-β, IL-1β and IL-6 for H9N2. The results will provide basic data for explaining inflammation differences and phenotype of histopathological changes caused by H5N1 and H9N2 and add new information on the role of chemokines and cytokines in viral virulence of AIVs.
[h=4]KEYWORDS:[/h] Avian influenza virus; Chemokines; Cytokines; H5N1; H9N2
PMID: 29577370 DOI: 10.1111/1348-0421.12588